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NiRs are divided into two different types based on their prosthetic groups, namely heme-containing nitrite reductases (cd1-NiRs) and Copper-containing nitrite reductases.

根据辅基的不同, NiRs分为血红素cd1型亚硝酸还原酶(cd1-NiRs)和铜型亚硝酸还原酶两种。

NiRs are divided into two different types based on their prosthetic groups, namely heme-containing nitrite reductases (cd1-NiRs) and Copper-containing nitrite reductases.

Cu-NiRs呈三聚体结构,每个单体都含有两种类型的铜原子,它们在酶催化过程中起着传递电子的重要作用。在催化过程中,Cu-NiRs中部分残基的结构变化有利于催化反应的进行。

A reactive phosphorus-containing compound, bis-phenoxy (3-hydroxy) phenyl phosphine oxide was successfully synthesized to produce the phosphorus-containing flame retardant epoxy resin.

合成了一种含磷二羟基化合物双(3-羟基-苯氧基)苯基磷氧化物,并在此基础上合成了含磷环氧树脂BHPPO-EP。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质粒,成功构建hTERT反义RNA表达质粒,转染SMMC-7721细胞后对其生长具有抑制作用。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针,筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库,得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase,SO)有60~90%的同源性,长度为647bp,编码140个氨基酸,具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20~40%的同源性,长度为1047bp,编码279aa,是一个不完整的开放阅读框,预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80~90%的同源性,长度为1810bp,编码493aa,具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30~70%的同源性,长度为1446bp,具有开放阅读框和BTB/POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框,与玉米Cat-3基因具有81%同源性,预测R8-1-1可能具有与Cat-3类似的功能,可为转基因小麦抗逆育种提供新的基因。

For the first three months of pregnancy, women should try to avoid perming hair, perming agents due to the nitro-containing compounds and alkaline hydrogen peroxide; especially hair dyes containing nitro-, amino-aromatic compounds, may affect fetal development, resulting in severe fetal deficiency occurred.

对于怀孕头三个月的妇女,应尽量避免烫发染发,因烫发剂中含碱性的硝基化合物和过氧化氢;特别是染发剂含有硝基、氨基的芳香族化合物,会影响胎儿的发育,严重的导致胎儿发生缺陷症。

The highest PLB multiplication rate was obtained on the media containing 2 mg/L of zeatin or BA, about 70% of the explants generating 16 or more PLBs in a month. The PLBs proliferated from the above experiment were subcultured one by one on the MS basal salt media with the organic compound made of yam bean or potato homogenate for shoot formation investigation. After 60 days, PLBs, from the zeatin treatment, subculture on the medium containing yam bean had the highest shoot formation rate.

进一步将上述试验所得之PLB单颗接种於含有80g/L豆薯或马薯的基本培养基中,比较有机添加物对PLB之芽体形成之影响,结果显示自2mg/Lzeatin处诱导所得之PLB,接种在豆薯培养基中有最高之芽体形成;而自IBA处中所诱得之PLB在豆薯及马薯培养基中皆有高比之芽体形成。

Methods Sixty male SD rats were randomly divided into six groups (n=10). Rats in water-drinking group were treated with deionized water, nifedipine group with deionized water and nifedipine 10 mg.kg-1.d-1, calculiinduced group with deionized water containing 1% ethylene glycol, and three calculi-induced+nifedipineintervening groups with deionized water containing 1% ethylene glycol plus nifedipine 3, 6 and 10 mg.kg-1.d-1, respectively.

60只雄性SD大鼠随机分成饮水组(只饮去离子水、硝苯地平组(饮去离子水,并灌喂硝苯地平10 mg.kg-1.d-1)、诱石组(饮1%乙二醇去离子水)、诱石+硝苯地平干预组(共3组,饮1%乙二醇去离子水,并分别灌喂硝苯地平 3、6、10 mg.kg-1.d-1),每组10只,连续处理4周。

The flame retardant molding compositions include an epoxy resin; a first transition metal oxide containing a refractory metal, such as tungsten trioxide; and a second transition metal oxide containing an oxyanion of a Group VIA element and a cation of a Group IIA element, such as calcium molybdate.

该阻燃模塑组合物包括环氧树脂、含高熔点金属的第一种过渡金属氧化物如三氧化钨;和含VIA族元素的含氧阴离子和IIA族元素的阳离子的第二种过渡金属氧化物如钼酸钙。

Based on the principle of molecular emission in hydrogen flame,sulphur-containing and phosphorus-containing substances in solutions were determined directly using self-made apparatus.

1前言硫化合物如甲硫醚等是造成环境水体中恶臭污染的主要原因之一[1],同样磷是有机磷农药、磷肥的主要成分,也是环境水质监测中的重要元素。

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There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式,可以避免教学形成模式後的僵化现象。

In addition, the mass transfer process of main components in the heat pump cycle, such as a generator and an absorber, is analyzed, and the mass trasfer model, the relation of its separation effectiveness with the thermodynamic inconvertibility are described.

根据热力学第一、二定律,从理论上建立了描述该类热泵的数学模型。从热力学不可逆性原理出发,分析了热泵循环过程的热力学不可逆性引起的〓损失。