查询词典 colone

The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of avain influenza virus subtype H5, was optimized in a reaction system and a condition to improve the sensitivity, specificity and accuracy. In addition, colone technology was used to develop a quantitative PCR format with virus copies amount.

针对H5亚型禽流感病毒血凝素基因保守区域设计特异性引物与TaqMan-MGB荧光探针，筛选并优化荧光定量RT-PCR反应体系与反应条件，用以提高方法的特异性、敏感性与准确性；并通过体外克隆技术建立病毒基因拷贝数进行定量分析。

Teamed up with four other beautiful and brilliant girls, all of whom are graduates of music academy schools, Sun Lili, herself a music academy graduate, founded the Five Princess Band in 2004. Unlike other girls bands which have been quick to colone the same style, an enormously success for a band of this nature, Five Princess is quietly insistent on developing its own style: combining singing with melody to create a new fashion of traditional Chinese music. The reason, according to Sun Lili, the singer of the band and one of the top ten winner singers in the Pepsi Pop Music contest, is that it greatly adds to the appeals of the music if the traditional melody is accompanied by a song.

&五乐格格女子新民乐弹唱组合&创建于2004年，由毕业于高等音乐学府的五位音乐学院青春靓丽，才华横溢的美少女组成，她们没有向如今遍地开花的其他新民乐组合一样去拷贝一种风格，而是走出了另外一条不同于任何组合的路线，那就是弹唱结合的形式，扬琴兼组合主唱手之一孙莉莉的想法是：现如今没有一首脍炙人口的器乐曲，很多器乐组合演奏了很多优美的器乐曲也不被广大听众认知，所以，她们认为有曲有唱，既有优美的民族乐器旋律，也会同时通过她们的演唱来丰富乐曲，这样才会流传下来她们的歌曲。

Teamed up with four other beautiful and brilliant girls, all of whom are graduates of music academy schools, Sun Lili, herself a music academy graduate, founded the Five Princess Band in 2004.Unlike other girls bands which have been quick to colone the same style, an enormously success for a band of this nature, Five Princess is quietly insistent on developing its own style: combining singing with melody to create a new fashion of traditional Chinese music.The reason, according to Sun Lili, the singer of the band and one of the top ten winner singers in the Pepsi Pop Music contest, is that it greatly adds to the appeals of the music if the traditional melody is accompanied by a song.

&五乐格格女子新民乐弹唱组合&创建于2004年，由毕业于高等音乐学府的孙莉莉召集了其他四位音乐学院青春靓丽，才华横溢的美少女组成，她们没有向如今遍地开花的其他新民乐组合一样去拷贝一种风格，而是走出了另外一条不同于任何组合的路线，那就是弹唱结合的形式，扬琴兼组合主唱手之一孙莉莉的想法是：现如今没有一首脍炙人口的器乐曲，很多器乐组合演奏了很多优美的器乐曲也不被广大听众认知，所以，她们认为有曲有唱，既有优美的民族乐器旋律，也会同时通过她们的演唱来丰富乐曲，这样才会流传下来她们的歌曲。

Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事，那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来，随着部分预应力砼梁应用的推广，发现我国规范所采用的名义拉应力法的计算结果不稳定。