查询词典 circulation of vector field

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

The System inset remote sense vector data and GIS vector data in drainage area into client programs programmed, realizing the application of remote sense vector data and GIS vector data in water resources management of Xinjiang Ma-na-si River irrigation area, and realizing the integrated management of the texts and maps of the water resources survey data, and spatial analysis and map edit functions of GIS as well. The system, which breaks away the platform of MapInfo, can run independently.

该系统将流域中的遥感栅格数据与GIS矢量数据集成嵌入到所编制的客户程序中，实现遥感数据和GIS数据在新疆玛纳斯河流域水资源管理中的应用，和水资源调查数据图文一体化的管理，同时具备了GIS的空间分析和图形编辑等功能，该系统能脱离Mapinfo软件平台独立运行。

Faraday's law is that when the flux in the conductor loop changes, it produces electromotive force in the conductor loop, and obeys to contour integral law , Maxwell Curl Theory is that when the magnetic intensity of a certain point in ether space changes, there produces electric field at that point in ether space, which is the field mutual production theory of "electric field produces magnetic field and magnetic field produces electric field" in the free space which everybody is familiar with, and it obeys differential law , Lorentz magnetic force is that when metal electrons cut magnetic lines, metal electrons forced by move along the conductor to form inductive current, in fact, it doesn't matter whether coil moves or magnet moves, only if there exists relative motion between magnetic field and conductor, metal electrons must cut magnetic lines, which is,"coil stills while magnet moves to the left" and "magnet stills while coil moves to the right" these two situations are the same, which both belong to metal electrons having cut magnetic lines.

法拉第定律指导体环路里的磁通量发生变化时，在导体环路上产生电动势，而且服从围线积分律；麦克斯韦旋度理论指以太空间某点的磁场强度发生变化时，在以太空间的该点产生电场，即大家熟知的自由空间里"电场生磁场与磁场生电场"之互生场理论，而且服从微分律；洛伦兹磁力指金属电子切割磁力线时，金属电子受力沿着导体漂移而形成感应电流，其实，无论线圈运动或是磁铁运动，只要磁场与导体存在相对运动，则金属电子必然切割磁力线，即，"线圈静止而磁铁向左运动"与"磁铁静止而线圈向右运动"这两种情况是一样的，都属于金属电子切割了磁力线。

This article analyses the application conditions of freezing method, current research status and the problems in thisiold. It, points that it" s necessary to study deep freezing temperature field deeply and discusses the factors which influence the freezing wall such as soil" s nature, moisture content, geological conditions, etc. It introduces the theory, process of freezing method, the distribution of temperature field of freezing method and key thermotics parameters influencing freezing temperature field, it simplifies the deep freezing temperature field model. Through ANSYS, this article founds the deep freezing temperature field model, and simulates the field on vary heat conductivities. It analyzes the results of ANSYS simulation, gains the theoretical value of thermometric holes which is needed by back analysis. Using the simulation temperature and actual value, it gets the equivalent heat conductivity. Then it solves the deep freezing temperature field by the equivalent value, simulates the freezing wall development process and temperature change curve. Against the actual project, the results are favorable.

文中分析了冻结法施工的应用条件，目前对冻结法施工的研究现状和在此领域内存在的问题，指出了对深土冻结温度场进行深入研究分析的必要性；对影响冻结壁形成发展的因素如冻土土性、含水量、地质条件及施工方法等因素进行了论述；对冻结法施工的原理、过程，冻结法温度场的分布情况，影响冻结温度场分布的主要热学参数进行了综述；对深土冻土温度场模型进行了合理的简化，通过ANSYS大型有限元分析软件，建立了深土冻结温度场的模型，对不同导热系数情况下的深土冻结温度场进行了模拟；对ANSYS的模拟结果进行了定性的分析，通过对ANSYS结果的后处理取得了反分析时需要用到的测温孔理论温度值；经过对测温孔模拟温度值和实测温度值的分析，得到了冻结温度场的等效导热系数；使用等效导热系数对深土冻结温度场进行了求解，模拟了冻结壁发展情况和温度场中的温度变化曲线，用所得结果对比工程实际情况，取得了较好的效果。

The introduction of torsion field can be included in a new meaning to the concept of physical vacuum . All kinds of fields (electromagnetic field, gravitational field and torsion field) can be regarded as the behaviors of physical vacuum under different polarized conditions. If one charged particle in vacuum spins to lead to the charge polarization of the vacuum , it takes the form of electric field ; if the move source is mass, by which the spin leads vacuum to the vertical polarization, it takes the form of gravitational field; If the spin is self-spinning to lead vacuum to the horizontal polarization, it takes the form of torsion field.

挠场的引入从最基本的层次上可以纳入对物理真空这一概念的新的理解上；各种场都可以看作为物理真空在不同极化条件下的表现；如果一个带电粒子存在于真空，作为一种挠动，使物理真空被电荷极化，就表现为电场；如果这种挠动源是质量，物理真空在质量的挠动下引起真空的自旋纵向极化，就表现为引力场；如果这种挠动是由于物体的自旋引起的，那么，真空被横向极化，就表现为挠场。

A mathemetical model of magnetic field in electromagnetic separation of inclusions from molten aluminum under rectangle coil and 50Hz current is presented according to the magnetic vector potential integral equation and the basic theory of magnetohydrodynamics. The magnetic field distribution in the gap of coils is simulated by the model and the actual magnetic field distribution also is measured. The experimental results show that the magnetic field distribution in the gap of coils is uniform and is consistemt with the model simulation. The magnetic field designed accord the simulated result is proved suitable in the practice.

摘 要：根据电磁流体力学的基本理论，利用矢量磁位积分，建立了在采用矩形电磁线圈和工频电源的条件下电磁分离铝熔体中夹杂的电磁场数学模型，模拟计算了电磁感应线圈气隙中的磁场分布状态，同时对制做的感应线圈的实际磁场进行了测量模拟计算磁场的分布状态与实际磁场的测量结果基本一致，都表明在电磁感应线圈气隙中磁场分布均匀，可以进行分离铝熔体中的非金属夹杂。

The approximate structure of nonlinear action of climatic atmosphere and ocean is: the nonlinear action decreases usually with increasing latitude, which is related to the role of the Coriolis force in fluid motion; the nonlinear action changes with the anomalous cyclonic or anticyclonic circulation shear, for instance, when the strength of anomalous eastward zonal circulation is comparable to that of anomalous meridional circulation, the nonlinear action is the strongest; wind stress plus gradient forces enhance the nonlinear action, etc..

气候海洋与大气非线性作用的大致结构是：通常与科里奥利力对流体运动的规范作用有关，非线性作用随纬度增加而减弱。距平流场的切变结构及其沿经向与纬向上强度的比较直接改变气候大气和海洋的非线性作用(比如，向东的距平环流强度与经向环流强度相当时，非线性作用最强)，较强的外部驱动使非线性作用加大等等。

In the past two years will not launch stock index futures, the full circulation of the mission only a first step, in order to avoid wasted, to achieve full circulation is of paramount importance, after the full circulation, the index will increase the difficulty of maneuvering, the only time The SFC will be improved at all levels in all varieties of time.

在过去两年将不会推出股指期货，特派团的全流通只是第一步，为了避免浪费，实现充分流通是很重要的全流通后，该指数将增加难度机动，唯一的时间，证监会将在一段时间各级各品种改良。

For finding out to agree with a point in the social responsibility of the business enterprise and the circulation the economic lawmaking, this selected works takes a market quasi- go into the system,manufacturer responsibility extension system,circulation economy evaluation the index sign system system,economic incentive system,the environment risk guard against system and across the profession coordination system to discuss how to enhance the business enterprise path of social responsibility in the circulation the economic lawmaking.

为了在企业的社会责任与循环经济立法中找到契合点，本文选取市场准入制度、生产者责任延伸制度、循环经济评价指标体系制度、经济激励制度、环境风险防范制度和跨行业协调制度，以论述如何在循环经济立法中强化企业社会责任的途径。

His female bullfighter Lydia gradually Health love, but she was in a vegetative state after the Bulls Zhuangcheng away from her, just because Lydia's ex-boyfriend showed more affectionate than he is like love.

他对女斗牛士莉迪亚渐生爱意，却在她被公牛撞成植物人后离她而去，仅仅因为莉迪亚的前男友表现出好似比他更深情的爱。

I know of none which are not easy to answer, but what I am trying to do now is only by a series of hints to throw some light on the narrow path of legal doctrine, and upon two pitfalls which, as it seems to me, lie perilously near to it.

我不认为有什么难以回答的问题，但我现在要做的仅仅是通过一系列线索，来给法律理论的狭窄路径以及在我看来离它近得危险的两个陷阱提供一些启示。