查询词典 chil

What kind of antipyretic do you usually prescribe to chil－dren who have got high fever?

你们一般 SsbbwW.com 给发高烧的孩子开什么药？

Objective To investigate the effect of air enema for diagnosis and reduction of intussusception in chil-dren.

目的 探讨空气灌肠整复法对婴幼儿急性肠套叠的诊断和治疗价值。

The results showed that pCI-ChIL-18 inoculation via allantoic cavity resulted in high level expression of ChIL-18 in nonage spleen and bursa of fabricius.

检测结果表明，胚胎接种pCI-ChIL-18使ChIL-18在鸡血液中早期持续高浓度存在，能促进鸡脾脏和法氏囊中ChIL-18基因的大量表达。

He knows the chil-dren who grow older each year, whose build, expression and appear-ance change—sometimes more pronouncedly, sometimes barely perceptibly.

他知道孩子每一年会长大，他们的身体、表情和面容也会改变。

Objective To observe the curative effect and side effects of fluticasone propionate inhalation in chil-dren with cough variant asthma.

目的 观察吸入丙酸氟替卡松治疗儿童咳嗽变异性哮喘的疗效和副作用。

Even those who had seemed asleep work up to watch the chil .

甚至那些已经向上似乎睡熟了的工作看 chil 的人。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

DNA序列测定表明，克隆得到的ChIL-18 cDNA与国外报道的完全一致，包括终止密码子在内其编码区的长度为510bp，编码169个氨基酸残基的蛋白质。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

Under the same conditions pET28a-△SIL-2 expression was higher. RNA structure software analysis showed that mRNA5'end sequences of pET28a-IL-2 formed a complex and relatively stable secondary structure, which testified that the existence of the signal peptide sequence had influence on the ChIL-2 gene in prokaryon expression.

RNAstructure软件分析表明，pET28a-IL-2的mRNA5'端序列形成了复杂且较为稳定的二级结构，不利于翻译的起始，证明信号肽序列的存在对ChIL-2基因在原核细胞中的表达有一定的影响。

I use an example quoted by Hu Jiaqi:"It is reported that America separates the DNA of a kind of virus by making use of genetic technology and combines it with another kind of DNA. Finally, they get a kind of virulent biological agent called a "pyrotoxin". Someone discloses in private that 20 grams of such a kind of biological agent could result in the global death of 6 billion people due to infection."

我在这里例举胡家奇所引用过的例子："据报道，美国利用转基因技术，将一种病毒的DNA分离出来，与另外一种DNA进行结合，拼结成一种剧毒的"热毒素"生物战剂，且私下有人透露，这种生物战剂只需20克，就可以导致全球60亿人全部感染死亡。"

Waiting, for the queers and the coons and the Reds and the Jews.

等待着疯子和黑人还有红色共产主义者还有犹太人