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The protease secreted from cellcould be more stable by adding the Ca2+ to fermentation broth. The cold-adapted protease, stable at 15℃, could be secreted in large quantity by the strainof SYP-A3-2. When the culture temperature was changed from 15℃ to 20℃, the strain grewfaster while the enzyme yield was decreased.

SYP-A3-2菌株在15℃下能大量分泌低温蛋白酶，且能有效保持酶的稳定性，在20℃时能以较快速率生长，但是酶量很少且酶活力不高，可以设计温度控制方案进行分阶段培养以缩短发酵周期。

As the nature of mass communication has changed and as information technology has evolved so too have the means, modes and subject matter of what used to be a much simpler subversive form of urban artistic expression.

由于大众传播的性质已发生变化，信息技术的发展也是如此的手段，方式和主题事项过去是一个非常简单的颠覆形式的城市艺术表达。

Be the rage of this method Ceng Jin, but the algorithm that searchs engine at present has changed such doing had been to won't do.

群发链接。这方法曾今风靡一时，但是目前搜索引擎的算法已经改变这样做已经是行不通了。

Be worn out I'll have to get my ticket changed.

我必须得更改我的票。

Due to the outbreak of World WarⅡin 1914 and his suffering from cancer, Debussy had to confront with both internal and external stress. His philosophy of life, even the philosophy of music changed. However, even in such an all time low of life, he had a beau ideal in these works.

而1914年第一次世界大战的爆发，加上当时作曲家本身所承受的身体疾患，使得他同时必须面对内在以及外在压力，以致於其人生观，甚至是音乐观均因而有戏剧性的改变。

He lived in the Marais, Rue des Filles-du-Calvaire, No. 6. He owned the house. This house has since been demolished and rebuilt, and the number has probably been changed in those revolutions of numeration which the streets of Paris undergo. He occupied an ancient and vast apartment on the first floor, between street and gardens, furnished to the very ceilings with great Gobelins and Beauvais tapestries representing pastoral scenes; the subjects of the ceilings and the panels were repeated in miniature on the arm-chairs.

他在二楼占用一套宽大的老式房间，一面临街，一面对着花园，大幅大幅的哥白兰①绒毯和博韦②绒毯挂齐天花板，毯子上织的是牧羊图，天花板上和壁框里的画缩成小幅，又出现在每张围椅上。

Bed linen is changed every 3 days.

所有的2岁以下的儿童的加婴儿床不需付费。

Can you have the towels and the bed linen changed every day?

你们可以每天换毛巾和床单吗？

I changed the bedclothes after I had slept in them for a week.

我每周都换一次我睡过的床上用品。

The economics have changed forever , and I suspect these kinds of sites will bedevil traditional media organizations .

经济已经永远地改变了，并且我怀疑这些站点将困扰传统媒体组织。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。