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SP-R mRNA in nasal mucosa, obtained from two groups, were used to do reverse transcriptive polymerase chain reaction and Southern blot.

以β-肌动蛋白作内标准，利用逆转录-多聚酶链反应(reverse transcriptive polymerase chain reaction， RT-PCR)，对变应性鼻炎模型组和对照组大鼠鼻粘膜SP-R mRNA进行了定量研究。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

Based on establishment of the human macrophage-derived foam cell model, the expression of Kv1.3 channel was investigated by immunocytochemical staining, reverse transcription-polymerase chain raction and Western blot. Furthermore, the effects of rMargatoxin, a Kv 1.3 channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein were studied.

在建立人巨噬细胞源性泡沫细胞模型的基础上，采用免疫细胞化学染色法、逆转录聚合酶链反应(transcription-polymerase chain raction，RT-PCR)及Western blot，研究Kv1.3通道的表达，并观察其特异性阻断剂--rMargatoxin对摄取氧化修饰低密度脂蛋白的巨噬细胞胆固醇代谢的影响。

Methods Using polymerase chain reaction-restriction fragment length polymorphism method, we detected 63 samples taken from 63 cases (including 36 invasive squamous carcinoma, 7 carcinoma in situ, 20 papillomas) for 248 point mutations in p53 gene.

采用聚合酶链反应――限制性片段长度多态性（polymerase chain reaction-restriction fragment length polymorphisms，PCR-RFLP）方法，对63例患者的送检标本（包括36例浸润性鳞状细胞癌，7例原位癌，20例乳头状瘤）中p53基因248位点的突变进行检测。

Our artefact supports the structuring and evaluation of RFID benets along business processes as we propose indicators for the derivation of an individual RFID cause-and-effect-chain.

我们支持构建和评价编造出来的RFID好处，以及业务流程为我们提出的指标进行了推导cause-and-effect-chain个体射频识别技术。

You may have a number of Back Buffers, this is known as a "Swap Chain".

你可能有很多的Back Buffer，这又称为&Swap Chain&。

You may have a number of Back Buffers, thellos is known as a "Swap Chain".

你有可能有很多的Back Buffer，这又称为&Swap Chain&。

You may have a number of Back Buffers, this is known as a "S wap Chain".

你可能有很多的Back Buffer，这又称为&Swap Chain&。

The Fire Island chain is patrolled by Undead corsairs, effectively blocking passage to Cantha.

Fire Island chain被不死系生物海盗占据，通往Cantha的路完全被阻隔。

Flash forward to the 1964 formation of his first "serious" band Thee Strangeurs, later renamed Chain Reaction, which enjoyed occasional moments in the spotlight as the opening act for The Beach Boys and the Yardbirds.

快进至1964年，他组建了他的第一个&系列&乐队 Thee Strangeurs ，后来乐队改名为 Chain Reaction ，乐队很享受偶尔在聚光灯下为沙滩男孩和 Yardbirds 做暖场乐队的时光。

It is also known as one of the most poisonous naturally occurring substances.

它也被称为一个最自然发生的有毒物质。

The greatest stress is found at the location on the cross section where V is the largest.

最大应力出现在横截面上V为最大的地方。