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Meanwhile we observed the change of symptoms, biopsy, T cell inferior group of serum (CD3、 CD4、CD8)、immunoglobulin (IgG、IgA、IgM) and the alexin (C3、C4), cAMP、 cGMP. CD3、CD4、CD8 are detected by immunomomoclonal antibodies APPAP; IgG、IgA、IgM are tested by immunoturbidimetry assay; the content of cAMP and cGMP in blood plasm are tested by radioimmunoassay.

所有观察病例于治疗前均作血、尿、大便常规化验；心、肝、肾功能检查；阴道分泌物检查包括念珠菌、滴虫、阴道清洁度；尿糖、血糖检查；分别观察中药组和西药组治疗前后相关症状及体征变化情况，活体组织病理检查的变化情况；血清T淋巴细胞亚群（CD3、CD4、CD8）和免疫球蛋白(IgG、IgA、IgM)以及补体（C3、C4）各项指标的变化情况；血中cAMP、cGMP含量变化情况，T淋巴细胞亚群水平测定采用单克隆抗体APAAP桥联酶标技术，血清免疫球蛋白IgG、IgA、IgM及补体C3、C4的测定采用免疫透射比浊法。

U0126 and TFP could suppress the appressorium production of S. turcica in glass slide and corn leaf surfaces independently or mixed-treated. The effect was dose-dependant and would be disappear when 500μM cAMP was added.

但加入cAMP后，它们的抑制作用消失，从而表明，MAPK途径与Ca〓-CaM途径和cAMP途径间即有协同作用，又相互影响，它们之间组成一个复杂的信号网络调控玉米大斑病菌的分生孢子和附着胞发育。

In this paper,the effect of cerium ion on cAMP and cGMP of primary hepatocyte and organs in rat was studied.

研究了Ce3＋对原代肝细胞内cAMP和cGMP的影响，并测定了用Ce3＋长期喂养的大白鼠脏器肝、肾、脾、肾上腺中cAMP和cGMP的含量。

Our results showed that hypoxia increased the Mb expression,but decreased cAMP and myocardial contractility.

结果表明，随低氧时间的延长Mb增加，cAMP和心肌收缩下降，Mb、cAMP和心肌细胞收缩频率经复氧可以得到恢复。

In additoin, the telencephalon and brain stem 5-H contents showed no marked changes, while diencephalon 5-HT content increased. The above experimental data and the other data in our department suggested that the changes of GnRH-GTH secretion might be related to the changes of hypothalamic neurotransmitters and EOP. The testicular cAMP changes might be secondary to those of the LH release.

从本实验及本室另外资料综合来看，GnRH-GTH分泌变化可能与下丘脑神经递质和EOP变化有关，睾丸cAMP含量变化可能继发于GTH变化，而睾丸TS合成和分泌又与GTH所致睾丸cAMP变化有关。

Results (1) Maximum bound volume and equilibrium dissociation constant of β2AR of lymphocyte in asthma patients at remission stage were markedly higher than that in normal subjects, while cAMP levels in blood plasma showed no difference.

结果缓解期哮喘患者外周淋巴细胞βAR的密度较正常人明显升高，血浆cAMP水平无显著差异，而发作期哮喘患者βAR及血浆cAMP水平均较正常人明显降低；缓解期哮喘患者外周淋巴细胞β2ARmRNA水平与正常人无显著差异。

The Thursday and Friday (July 10-11) before this year's Foo Camp in Sebastopol July 11-13, O'Reilly AlphaTech Ventures will be hosting OATV Startup Camp .

O'Reilly AlphaTech风投将会搞一个 OATV 新创公司训练营活动，安排在Sebastopol今年 Foo Camp （7月11-13日）之前的周四和周五（7月10-11）。

A dose-dependent inhibition of cGMP-PDE activity was observed when cUMP 10~(-6-10~(12) M was added to the culture medium, which may explain the increase of the level of intracellular cGMP, while the activity of cAMP...

浓度为10~(-6)-10~(-12)McUMP对胞内cAMP-PDE活性几乎没有影响，而抑制cGMP-PDE的活性，抑制作用随cUMP浓度降低而减小，这可能是胞内cGMP水平增高的原因。cUMP可能与细胞的cAMP PK结合，释放催化亚单位，细胞生长的抑制可由此发生。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

PKA activators such as cAMP, 8 - Br -cAMP or phosphodiesterase inhibitor isomethyl butyl xanthine or purified PKA catalytic subunit all can inhibit germinal vesicle breakdown and meiotic maturation in mouse oocytes, also in Xenopus oocytes.

以小鼠卵母细胞为实验对象，给予PKA激动剂cAMP、8-Br-cAMP或磷酸二酯酶抑制剂IMBX均可抑制小鼠卵母细胞的胚泡破裂及减数分裂成熟。

The physiology of the skin machine body for the sake of the environment in the maintenance stability, but eject a body metabolism creation of metabolism thing BE'grease', summer perspire many various skin disease light or good, autumn perspire little metabolism thing metabolism don't go out, various skin disease made, this be the skin machine body of various burning disease.

皮肤机体的生理为了维持内环境稳定，而排出身体代谢产生的代谢物是'油泥'，夏天出汗多各种皮肤病就轻或好了，秋天出汗少代谢物代谢不出去，各种皮肤病就犯了，这就是皮肤机体的各种炎症。

If I had anything tender in me, I shot it dead.

如果我有什么招标的话，我枪死亡。