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2B8a was weakly reactive to neutrophils (23.72%) and negative for T cells, NK, DC, RBC and Plt. The antibody reacted to all 3 marrow CD34+ cells with an average positive rate of 39.33% while it was negative for G-CSF-mobilized CD34+ peripheral blood stem/progenitor cells (PBSC, 1.25%). Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34+细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34 细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

Second order incident boundary condition on fixed incident boundary is derived for numerical simulations, based on the cnoidal or sinusoidal motions of wave maker paddle, which shows that the prediction with second order incident boundary condition is more accurate than the prediction with first order incident boundary condition. 2 The analytical solution for higher-order Boussinesq equations is derived and its applicable range is discussed. 3 A 2-D fully non-linear numerical model using boundary element method is developed to obtain wave forces acting on rectangular obstacle. 4 The three-dimensional fully non-linear waves are studied in a numerical wave tank using finite element method.The studies on the coupled numerical model combined Boussinesq equations with Laplace equation are following.

非耦合非线性波浪计算模型：1 根据造波板做椭圆余弦运动或正弦运动速度，推导出数值模拟波浪水槽时固定入射边界上的二阶波浪入射边界条件，数值计算结果和实验结果的对比表明采用二阶入射边界条件对波面升高的预报比采用一阶入射边界条件对波面升高的预报更为精确；2 推导了波浪水槽造波板做正弦运动所产生波浪的高阶Boussinesq方程摄动展开解析解，讨论了该解析解的适用范围；3 对整个波浪水槽应用边界元方法数值模拟了波浪对物体的非线性作用；4 用有限元法求解三维Laplace方程模拟了三维完全非线性波浪水槽。

With the help of the fundamental theory to singular measure differential system, Green's function matrix expression of solution and well-posed boundary condition are given, together with discussing the properties of the Green's function matrix under the influence of impulsive effect In section 5, based on Lyapunov function method, we investigate that the existence of the first boundary value problem to second-order singular system by macthing the solutions to the second boundary value problem; and the existence and uniqueness of three-point boundary value problem to third order singular system by macthing the solutions to two-piont boundary value problem.

第五部分借助Lyapunov函数法，通过将第二类边值问题的解对结成第一类边值问题的解，得到了二阶广义系统的第一类边值问题解的存在性；通过将两点边值问题的解对结成三点边值问题的解，得到了三阶广义系统的三点边值问题解的存在性和唯一性。

New boundary integral equations for the torsion problem of an isotropic bar with curvilinear cracks are derived by using integration by parts and other techniques to the traditional boundary integral formulations. And the boundary integral equations for the torsion problem of an anisotropic bar with curvilinear cracks are also derived by using the isotropicalized boundary element method. Then the anisotropic problem can be solved on the base of the boundary element method of isotropic problem.

然后用各向同性化方法，将各向异性材料裂纹柱体的扭转问题转化为各向同性材料的问题，在各向同性材料裂纹柱体扭转问题的新的边界积分方程的基础上，推导出了适用于各向异性材料裂纹柱体扭转问题的边界积分方程，然后按已推导出的各向同性问题的边界元法求解。

LBIE, based on the local boundary equation, adopts the traditional moving least squares approximation which depends on only the values of the nodes in the domain of the problem or along its boundary. The whole process of integration is carried on over a local domain or its local boundary centered at the node in question. The local boundary equation can be rewritten to represent the values of the unknown function at the point of interest, and the essential boundary conditions can be directly and easily enforced by using the Green formula and the characters of the Dirac function.

它以局部边界积分方程为基础，采用移动最小二乘近似函数，从而只需要分布在问题域内及其边界上的节点的信息值，无需划分单元；整个积分是在以节点为中心的局部域及其边界上实现，所以不需要背景积分网格；借助于格林公式及Dirac函数的性质，将局部边界积分方程转化为所考虑点的未知函数的边界积分表达式，便于直接施加本质边界条件。

According to the different orientations of domain walls with respect to the (100) observation plane of a foil, 90°domain boundaries may be divided into two kinds. one is thin lens or ring shaped a-a 90°domain boundary, and the domain wall lies on the {110} plane; Usually, most of these domain boundaries are uncharged; according to the extinction rules for the (011) domain walls, the displacement vector 〓 along 〓 can be dertermined, which is parallel to the line of intersection between the domain wall and the plane consisting of spontaneous polarization vectors on both sides of domain boundary; from selected area electon diffraction of crystals including domain boundary, we not only examine slight difference in the crystal parts on both sides of the domain boundary, but also obtain crystallographic relationship between them, that is, there exists a 180°rotation symmetry operator along a normal of domain wall. The other is Z or ring shaped a-c 90°domain boundary along {010} direction, and the displacement vector 〓 runs along 〓 direction.

根据界面相对于样品中(100)观测面取向的不同，90°畴可分为两类：一类为薄透镜片状或环状的a-a90°畴，其界面平行于{110}，多数不带电，由消光规律可确定其位移矢量平行于畴界面与自发极化所在平面的交迹，即，〓方向，而从包含畴界的选区电子衍射图可知，这种不带电的90°畴界两侧存在微小的取向差，其界面两侧的结晶学关系为以界面法线为二次轴的旋转关系；另一类为Z状或环状的a-c90°畴，其界面平行于[010]或[001]，相应的位移矢量为〓方向。

In Chapter two, it is given of the three-dimensional boundary element and the boundary element space, Sobolev space for the contact boundary element problem with friction; it is proved of existence and uniqueness of the solution by variation inequality for the contact Boundary Element Method matrix equation under two kinds of condition that the contact zone is fixed and the contact zone is increasing along with the load; it is given of the expressionfor the error of the exact solution and the boundary element solution.

第2章，给出三维边界单元及边界元空间、有摩擦接触边界元问题的Sobolev空间，利用变分不等式证明了不变接触区和随载荷可变接触区两种情况下的接触边界元法矩阵方程解的存在唯一性，给出了准确解与边界元解的误差表达式，还证明了三维弹塑性摩擦接触问题凝缩矩阵解的存在唯一性，为三维弹塑性摩擦接触边界元法奠定数学基础。

For the third boundary value problem under ellipsoidal boundary, firstly we simplify the form of the third boundary value problem. Then, the solution of the the third boundary value problem under ellipsoidal boundary is discussed in detail, along with its series solution, the integral expression is mainly studied.

对于椭球界面下的第三类边值问题，先简化其形式，然后详细讨论了椭球界面下简化形式的第三类边值问题的求解方法，在给出级数解的同时，主要研究了它的积分解的表达式。

Coordinate sequence method is made up of the instruct information of polygon boundary x, y coordinate. It is the easiest polygon vector encoding method. The file structure of this method is easy, but because of the polygon boundary has stored two times, it will produce data redundancy, moreover, short of neighbor threshold information; Tree index encoding method make all the boundary points digitizing, store the coordinate sequence, connect the point index to boundary line, and then connect the line index to other polygons, so as to the tree index structure has came into being. It eliminates neighbor polygons boundary data redundancy problems; Topology structure encoding method is a method which by setting up a complete topology relationship structure, resolve the neighbor threshold and island information process problem. However, it makes arithmetic more complicated and makes database bigger.

坐标序列法是由多边形边界的x，y坐标对集合及说明信息组成，是最简单的一种多边形矢量编码法，文件结构简单，但多边形边界被存储两次产生数据冗余，而且缺少邻域信息；树状索引编码法是将所有边界点进行数字化，顺序存储坐标对，由点索引与边界线号相联系，以线索引与各多边形相联系，形成树状索引结构，消除了相邻多边形边界数据冗余问题；拓扑结构编码法是通过建立一个完整的拓扑关系结构，彻底解决邻域和岛状信息处理问题的方法，但增加了算法的复杂性和数据库的大小。

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗，但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉！