查询词典 blastocyst

The gene targeted mice are commonly produced by breeding the heterozygous targeted mice following microinjecting the homologous recombinant ES cells into the blastocoele cavity of 3.5dpc embryos at the blastocyst stage to give birth to chimeric mice.

传统基因敲除获得某一突变基因的纯合体动物首先要利用打靶胚胎干细胞获得嵌合体动物；随后用嵌合体动物与野生型个体交配得到突变基因的杂合体动物；最后才能通过杂合体动物之间的交配来生产纯合体动物。

The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

试验获得了如下结果：1。以山羊耳成纤维细胞为供体，以牛、羊、兔卵母细胞做为胞质受体构建重构胚，并经体外培养，发现不同的胞质受体均能支持重构胚的早期发育，但受体胞质对重构卵的重构胚早期发育速度和卵裂率均有显著影响。

The results show that GSH can improve the development rate of morula or blastocyst (32.84%),and rate of blastocyst (26.35%).

这表明GSH对牛胚胎的发育有一定的保护和促进作用。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

结果：与无上皮细胞相比，共培养明显促进了小鼠桑椹胚率（71.3%和61.2%，p.05），囊胚率（50.0%和39.7%，p.05），囊胚孵化率（34.8%和24.3%，p.05）和胚胎总细胞数（44.1和35.6，p.05）。结论：子宫内膜上皮细胞能够促进小鼠胚胎的体外发育，对早期胚胎的体外发育环境具有优化作用。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

These experiments demonstrated that hyperglycemic conditions in vitro can inhibit the growth and development of the embryo cells resulting in the down-regulated expression of igf-1, and IGF-1 could inhibit the apoptosis of blastocyst and be benefit for the growth of blastocyst.

因此，高血糖能抑制胚泡细胞的生长发育，导致igf-1的表达下调，而IGF-1能抑制胚泡细胞的凋亡，有利于胚泡细胞的生长发育国。

The effects of different temperatures (37, 38, 39) on parthenogenetic embryo cleavage and blastocyst rate and the effects of different time (2, 3, 4 h) on parthenogenetic embryo cleavage and blastocyst rate were studied.

方法］研究不同温度(37、38、39℃)对孤雌胚胎卵裂率、囊胚率的影响；不同时间(2、3、4h)对孤雌胚胎卵裂率、囊胚率的影响。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

Blastocyst rate of embryos co-cultured with pig oviduct epithelium cells came from pig oviduct during diestrus was significantly lower than metestrous. 4. The blastocyst rate of transgenic embryos collected at 24~26 hours and 18~22 hours after hCG-treated reached 45% and 20% respectively.

实验证明在hCG注射后24~26h之间获得的受精卵，在原核显微注射人血清白蛋白基因后体外培养，有45%注射胚发育到囊胚阶段，而在18~22h仅有20%，二者差异显著（P.05）。5。

Results The expression of endogenous LN was found in the basement membrane of epithelium, glands and blood vessels in uterine endometrium of both estrus and early pregnancy. And its was faint that LN present in basement membrane as the implantation occurs, but the LN immunostaining in the epithelial cells of endometrium was stronger. The exogenous LN has no obvious influence on blastocyst attachment and implantation. LN-antibody intra-uterine injection showed significant repressing effect on mouse blastocyst implantation.

结果 动情期及妊娠早期小鼠子宫内膜内源性LN在上皮和腺基膜及血管内皮基膜均有表达，并随着植入的发生基膜的LN表达减弱，而内膜上皮中LN免疫染色逐渐增强；外源性LN对小鼠胚泡粘附与植入子宫内膜不产生明显影响，层粘连蛋白抗体明显抑制小鼠胚泡着床。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。