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ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果：hMSCs诱导前为纺锤形，诱导后第2天部分细胞即开始发生形变，呈球形或短棒状，1周后胞浆中颗粒增多，约20～30%细胞边缘呈毛刷样变化；hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义，CD90未诱导时表达呈弱阳性，诱导后明显增高(P.05)；ANP和CX43在诱导前无表达，诱导后第2周开始表达且表达随时间逐渐增强，但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加，低浓度诱导组低于标准浓度诱导组，组间差异有统计学意义(P.05)，G_0/G_1期细胞比例诱导后较对照组显著减少(P.05)；β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强，在第6周时均达到高峰，第8周时表达开始衰减，Bcl-2、Bax基因表达呈时间依赖性变化，hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

Before you see the plastic surgeon, before you plunk down half apaycheck on that pricey anti-aging cream, in fact, before you do anything, put on sunscreen.

在你去看整形医生之前，在你花掉一半薪水在昂贵的抗衰老霜上之前，事实上，在你采取一切行动之前，先涂上防晒霜。

The LORD hears us before we call and often answers us in the same speedy manner. Foreseeing our needs and our prayers, He so arranges providence that before the need actually arises He has supplied it, before the trial assails us He has armed us against it.

我们尚未求告，祂就知道我们的需要，应允我们的祷告，供应我们一切所需；在试验尚未临到我们之前，祂就装备我们，好让我们能应付磨难。

The highest fruit set percentage was obtained when hand-pollination was done in one day (at the stage of half opened petals) before the anthers naturally dehisced. Hand-pollination could increase fruit set from 2 days to 4 hours (at the stage of tiny dehiscent petals to over half-opened petals) before the anthers naturally dehisced. The pistil compatibility was strong and fruit set percentage with hand-pollination was the highest one day before the anthers naturally dehisced (at the stage of half-opened petals).

从花药自然裂开前2 d到自然裂开前4 h（相当于花瓣呈微张开到超过半张开状态），雌蕊都具有容受性，这期间进行人工授粉能提高坐果率，但以花药自然裂开前l d（相当于花瓣呈半张开状态时），雌蕊容受性最强，人工授粉坐果率最高。

"I have you both beaten," the lawyer gloated."Before Adam and Eve, before the Garden of Eden, before all creation, there was a state of chaos, and who do you think could have created that?"

&你们俩的都不如我的早，&律师得意地说：&在亚当和夏娃之前，在伊甸园之前，在所有这些创造之前，有个混沌的世界，你们知道，是谁制造了那个混沌的世界吗？&

Investigation shows, in electronic government preparation is spent (the website prepares to spend, infrastructure prepares to spend reach manpower resource to prepare to spend) exponential respect, with respect to the whole world 5 continent, europe unplugs with 0.649 component of force the head prepares; to be in before the rank in 35 countries, european country occupied 70%; to be in before the rank in 10 countries, european country was occupied 60%, especially boreal Europe the Three Kingdoms it is before undertaking the whole thing 3 armour more, gradually hasten is become worldwide inside of electronic government trend bring the person that run newly.

调查显示，在电子政府预备度(网站预备度、基础设施预备度及人力资源预备度)指数方面，就全球五大洲而言，欧洲以0.649分力拔头筹；在排名前35名国家中，欧洲国家占了70%；在排名前10名国家中，欧洲国家占了60%，尤其是北欧三国更是包揽了前三甲，渐趋成为世界范围内电子政府潮流的新引跑者。

Products in the rig before the final factory test, to ensure that the issues raised in the process of testing, inspection record card on the failed item, purchase inspection problems be resolved before leaving the factory, out of the failure of all single at the factory be closed before the. 2005.7 ~ 2008.4 Assembly Technology division Chengdu Aircraft Industrial Group Co., Ltd.

5，在钻机出厂前进行产品的最终检验，确保过程检验中提出的问题、检查记录卡上的不合格项、进货检验中出现的问题在出厂前得到解决，所有开出的不合格单在出厂前得到封闭。

The technical measures to improve packing quality of bottled liquor mainly include online examination of packing materials before liquor packing, sampling and assaying before liquor bottle-filling, and examination of liquor bottles before liquor-filling on the production line etc.

提高瓶装白酒包装质量的主要措施有：包装前对包装材料进行上线检验，酒在灌装前再次进行取样化验，酒瓶在上线灌装前进行检查等。

Products factory operators; Steyr, Steyr Wang, Hao Wo main bridge shell rear axle, bearing blocks, cross the bridge cover, Steyr wheel shell, shift cylinder, rear axle sudden yuan, ZF Clutch fork shaft, swing rod, before and after the brake hub, Steyr various separation fork, gear room covered ,67-68 pulley, bridge sudden yuan, steel plate, parallel to shell axis, double-H shift shaft , 0503 Steyr-axis between the shell bad, bad Steyr 0198 Inter-axis shell, oil seal and seat, steel plate before and after the hanging ears, rear wheel, shift box assembly, double H shift rocker, one, two block I. block F99588, shift box casing, drive shaft telescopic forks, steel slide, shift cylinder, a variety of connecting plates, gearbox sudden yuan, three, blockIntroduction F99589, Steyr cylinder cover, double-H shift dial head, adjust the lever fork, wheel cover, FL clutch fork, FL Clutch fork shaft, Steyr after the output shaft bearing support, flywheel shell, fork and seat connection, Steyr Cylinder Head,ácover ,67-68 water pipe joints, rear brake camshaft bracket, rear axle minus the main shell, minus the shell of the main bridge, bearing seat, adjust the lever forks, brackets, brake lane joystick arm, gearbox sudden yuan, clutch pedal arm, oil block at the end of care, single head, double-headed rocker, gear room covered, decelerationround, adjust the lever forks, shift box casing, double H shift dial head, Double H shell, gear room covered, separation lever plate before and after the hanging ears, double-H shift shaft, decelerationround,áBushing, Steyr 10 file clutch shell, the bridge box, minus the shell after the main bridge , Bearing Block, the bridge cover, Steyr wheel shell, shift cylinder, after the sudden the bridge element, ZF Clutch fork shaft, swing rod, steel slide, shift box casing series products .

本厂经营产品；斯太尔，斯太尔王，豪沃后桥主桥壳，轴承座，过桥箱盖，斯太尔轮边壳，换挡汽缸，后桥突元，ZF离合器拨叉轴，摆杆，前后制动毂，斯太尔各种分离拨叉，齿轮室盖，67-68皮带轮，中桥突元，钢板垫板，平行轴壳，双H换挡轴，斯太尔0503轴间差壳，斯太尔0198轴间差壳，油封及座，钢板前后吊耳，后轮毂，换挡盒总成，双H换挡摇臂，一，二挡导块F99588，换挡盒套管，传动轴伸缩叉，钢板滑座，换档汽缸，各种连接盘，变速箱突元，三，四挡导块F99589，斯太尔汽缸盖，双H换挡拨头，杠杆调整叉，轮边盖板，FL离合器拨叉，FL离合器拨叉轴，斯太尔输出轴后轴承座，飞轮壳，连接叉及座，斯太尔汽缸盖（欧II，平衡轴盖板，67-68水管接头，后制动凸轮轴支架，后桥主减壳，中桥主减壳，轴承座，杠杆调整叉，托架，行车制动操纵杆臂，变速箱突元，离合器踏板臂，油底托块，单头，双头摇臂，齿轮室盖，减速轮垫片，杠杆调整叉，换挡盒套管，双H换挡拨头，双H壳体，齿轮室盖，分离杠杆，钢板前后吊耳，双H换挡轴，减速轮垫片，平衡轴衬套，斯太尔10档离合器壳，过桥箱，后桥主减壳，轴承座，过桥箱盖，斯太尔轮边壳，换挡汽缸，后桥突元，ZF离合器拨叉轴，摆杆，钢板滑座，换挡盒套管等系列产品。

She had come to Middle Cave five years before, but felt a sense of loss on this return."It's not as pure as before. Before all the houses were neat and clean, it really looked like a little village." Now every house has changed, for example, Wang Fengzhong has hung a "Country-style food" sign outside his gate, indicating the price, and has laid down a concrete floor.

她5年前来过中洞，这次重返却感到失落，&没以前那么纯粹了，以前房屋整齐、干净，真像一个小村庄&，现在每家房子都有了区别，例如王凤忠，他门前挂起&农家饭&的牌子，标明价钱，还铺了水泥地。

On closer examination, though, this is not a vote for multilateralism but just the opposite.

仔细审视后我们发现，这并非是对多边主义投出的赞成票，而是恰好相反。

Uncovering their weak spots, so I can defeat them.

揭露出他们的弱点，这样我就可以打败他们了。