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The development of ba sic science such as Mathematics Statistics,Gentics and etc,brought about the ra pid development of applied science such as Group Gentics,Quantitative Gentics,Mo lecular Gentics and so on.Afterwards,many pig breed selection methods such as ap pearance selection,gentic parameter application,index selection,BLUP,gentic mark selection and etc.appearad.Then these methods have been widely applied in bre eding by now.

而数学、统计学、遗传学等基础学科的不断发展，带动了群体遗传学、数量遗传学及分子遗传学的发展，猪的表型选择、遗传参数的应用、指数选择、BLUP法、遗传标记辅助选择等多种选种方法相继问世，并在猪育种实践中得到广泛应用。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

Methods:The proliferation inhibition of cantharides injections on LoVo cells were detected by tetrazolium.Annexin V-FITC were applied to study cell apoptosis of LoVo cells.FCM were applied to detect the expressions of the Fas and Bcl-2 protein.Results:Cantharides iniections could inhibit the proliferation of LoVo cells and could couse cell apoptosis.

四甲基偶氮唑盐测定药物对LoVo细胞抑制作用与时间和剂量的关系，Annexin V-FITC方法对细胞凋亡进行检测，流式细胞术检测细胞Fas、Bcl-2蛋白表达水平结果：MTT实验表明复方斑蝥注射液可抑制LoVo细胞的生长并诱导发生凋亡。

According to the relative literature, this paper surveys the taxonomical adscription, the main botanic characters and ornamental characters of the Malus and Chaenomeles species which have been used or have potential applied value in landscape architecture, summaries the expressional forms of their culture, states their disposition and applied forms in the classical and modern landscape, points out the problems that lie in the development and application of them in landscape, raises the exploitation measures and forecasts the application prospect.

依据相关文献，概述了中国园林绿化中已经应用或有潜在应用价值的海棠树种资源的分类学归属、主要植物学特征和观赏特征，总结了海棠文化的内涵和表现形式，阐述了海棠树种在古典和现代园林中的配置方式和应用形式，指出了海棠树种资源在园林应用开发方面存在的问题，并提出开发对策，展望了应用前景。

The electron microscope scanning results showed that dense mycelia and conidial colonies were observed on the leaves of non K2SiO3-applied plants, while loosened mycelium or no mycelium on the leaf surface of K2SiO3-applied plants.

经电镜扫描分析显示，诱导接种后，未加硅酸盐的叶片布满浓密的菌丝体及分生孢子团，经硅酸盐处理的叶片上仅有松散的菌丝或者没有菌丝分布。

Methods Suturing coracoclavicular ligament after clavicular hook plate fixation was applied in treating acromioclavicular dislocation in one group of 23 cases ;in the other group of 24 cases the suturing was not applied .

应用锁骨钩钢板固定并缝。。。目的比较锁骨钩钢板固定后缝合喙锁韧带和不缝合喙锁韧带两种方法治疗肩锁关节脱位的临床效果。

Methods Suturing coracoclavicular ligament after clavicular hook plate fixation was applied in treating acromioclavicular dislocation in one group of 23 cases ;in the other group of 24 cases the suturing was not applied .

应用锁 。。。目的比较锁骨钩钢板固定后缝合喙锁韧戴煳不缝合喙锁韧戴两种方法治疗肩锁关节脱位的临床效果。

Methods Suturing coracoclavicular ligament after clavicular hook plate fixation was applied in treating acromioclavicular dislocation in one group of 23 cases ;in the other group of 24 cases , the suturing was not applied .

应用锁骨钩钢板固定并缝合喙锁韧带治疗肩锁关节脱位23例，锁骨钩钢板固定后不缝合喙锁韧带治疗肩锁关节脱位24例。

Methods suturing coracoclavicular ligament after clavicular hook plate fixation was applied in treating acromioclavicular dislocation in one group of 23 cases ;in the other group of 24 cases , the suturing was not applied .patients were followed up for 18-62 months with the mean of 37 months .

应用锁骨钩钢板固定并缝合喙锁韧带治疗肩锁关节脱位23例，锁骨钩钢板固定后不缝合喙锁韧带治疗肩锁关节脱位24例。随访18～62个月，平均37个月。

Ypex Modified can be applied as a second coat to reinforce Xypex Concentrate, or applied by itself to dampproof the exterior of foundation walls.

YPEX增效剂可以用作XYPEX浓缩剂的第二层涂料，或用于基础墙的外表面以起防潮作用。

If you were not , and OS X just booted normally, have no fear.

如果你没有，和OS X刚刚启动通常，没有恐惧。

I only want to preserve our all .

我只想保存好我们的一切