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Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans. Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common Pathogenic non-Candida albicans (Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis), and the products were digested with the two restriction endonucleases (Msp Ⅰ and Hae Ⅲ) respectively. Results The four isolates consisted of 15 strains of Candida glabrata (7.50%), 7 strains of Candida parapsilosis (3.50%), 5 strains of Candida krusei (2.50%), 2 strains of Candida tropicalis (1.00%).

首先采用芽管试验、厚壁孢子试验、法国科玛嘉念珠菌显色培养基及API20CAUX酵母菌鉴定系统将分离自VVC患者阴道内的念珠菌菌株鉴定到种，然后采用真菌通用引物将4种常见非白念珠菌（包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌）进行PCR扩增，并选用MspⅠ和HaeⅢ两种内切酶对扩增产物进行酶切分析。

After circularization by self-ligation, inverse polymerase chain reaction is used to amplify the integration sequences of the human high metastatic potential hepatocellular carcinoma cell line MHCC97-H genome.

对酶切片断进行自身环化后，运用反向聚合酶链反应对人高转移潜能肝癌细胞株MHCC97-H基因组中整合序列进行扩增。

Fifty-three primer combinations were found to amplify nuclear DNA of Chinese cabbage, properly. They amplified the fragments up to 4 381 with an average of the 83. Among them, 9 markers derived from 9 primer combinations were linked to the clubroot resistance gene.

筛选出的适宜53对引物组合共扩增出4381条带，占总扩增片断的33.50%，平均每对扩增出83条，其中9个组合扩增出与抗根肿病基因连锁标记。

Based on the sequence of the codominant RAPD marker〓,we designed SCAR primers SCN11 to amplify distinct band〓 with the same size as RAPD marker 〓.

根据克隆片段〓和〓的序列设计合成了一对SCAR引物SCN11，SCN11在95-5383中扩增出与〓大小相同的片段〓，在HB1中扩增出与〓大小相同的片段〓，在F1同时扩增出〓。

The key components in laser gyro is He-Ne ring laser, and the reciprocal action theory between light and medium is the base in studying the ring laser, use the Lamb half classical theory, and on the base of density matrix theory, the optical Brounch equation was established, which described the movement of amplifying medium atom in the ring syntonic cavity, and according to the medium polarize theory the self consistent equations was deduced, which describe the light intensity and phasic of the counter-propagating wave. On the basis of this equations, adopted the simulation software MATLAB and virtual instrument programming language LabWindows/CVI, we can do some simulated experiments in study the phenomenas such as amplify and dispersive characteristic of medium, mode pushing effection, burned hole effection and pattern competition, lock-in of frequency and the characteristic of light intensity and phasic of the counter-propagating wave in laser gyro.

激光陀螺的核心部件为He-Ne环形激光器，而掌握光与介质的相互作用理论是研究激光器的关键，采用拉姆半经典理论为主，在密度矩阵理论的基础上，推导环形谐振腔中描述He-Ne气体增益介质原子运动的光学布洛赫方程，运用介质极化理论得出描述激光陀螺反向行波的光强、位相所满足的自洽场方程组，在此基础上，运用MATLAB仿真软件和虚拟仪器编程语言LabWindows/CVI，对激光陀螺中的介质增益色散特性、频率牵引效应、烧孔效应及模竞争、闭锁效应及环激光的光强和相位特性进行仿真试验研究，并且运用全量子理论，对激光工作原理进行分析，得出二能级系统单模辐射场的光子数密度分布，得出激光场的光子统计分布，仿真激光场的动态建立过程。

Currently, it must amplify the socialistic democracy, construct socialistic state with rule of law, and develop socialistic political civilization.

当前，必须扩大社会主义民主，建设社会主义法治国家，发展社会主义政治文明。

With this new control element, a circuit could amplify, oscillate, and switch.

利用这种新的元件，电路能够实现放大、振荡和开关。

Objective To amplify and clone Cysticercus cellulosae AgB cDNA coding region.

扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。

PCR is one of the common techniques in molecular biology, which can amplify nucleic acids through the cycle of denaturation, annealing and extension.

PCR是最常用的分子生物学技术之一，通过变性、退火和延伸的循环来完成核酸分子的大量扩增。

The conserved regions of rRNA and tRNA genes exhibited higher sequence similarity among plant species. Five primer pairs were designed from the conserved regions distributing in psbA~trnK, psbB~psbH, rpl23~trnI, trnR-ACG~trnN-GUU and trnY-GUA~trnD-GUC, respectively to amplify sequences about 500~600 bp, 800~900 bp, 650~700 bp (350 bp, not legume species), 700~800 bp (400 bp in dicotyledon), and 500~600 bp.

经由上述比对分析后设计出5引子对，位於叶绿体基因体的位置分别为psbA~trnK、psbB~psbH、rpl23~trnI、trnR-ACG~trnN-GUU及trnY-GUA~ trnD-GUC，预期实际应用在其他物种上所能增幅之变异序列长度分别约为500~600 bp、800~900 bp、650~700 bp (豆科以外物种为350 bp)、700~800 bp (双子叶植物为400 bp)及500~600 bp。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。