查询词典 amplification

As high sensitiveness of ABI3730XL DNA analyzer, common PCR amplification reaction system was not suitable for the detection system.

由于ABI3730XL DNA分析仪的灵敏性很高，普通的PCR扩增反应条件不适应其检测系统。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

The effect of pulse width on soliton amplification has been studied.

为了获得更短的光脉冲，脉冲放大技术已成为研究的热门课题。

Stagger studded for tremendous soundproofing and meticulously designed and constructed to allow perfect amplification, the sound quality is incredible without disrupting adjoining spaces.

隔音墙带来惊人的隔音功能，加上精工设计打造，造成完美的放音效果，不可思议的声音品质，却不致干扰到邻近空间。

Some classical applications and research progress are introduced, such as, selective amplification, frequency multiplication, up-conversion and signal generation in the terms of the gain spectrum as well as the carrier suppression and phase shift from the loss spectral point of view.

从受激布里渊散射增益谱的角度对微波光子信号的选择放大、倍频、上变频和信号产生以及从损耗谱的角度对信号载波抑制和信号相移等典型应用和研究进展进行了介绍。

Results Stable Spectral Karytyping method was set up and extraordinary multiplicity of structure, including metathesis、depletion、amplification、rearrangement、equiarm、marker-body and so on were found by this methad.

结果 我们建立了稳定的光谱核型分析方法，发现肿瘤实体瘤染色体异常呈多倍体且结构异常复杂，包括易位、缺失、扩增、重排、双着丝粒、等臂及标记染色体等。

The lack of amplification in these tubes could be caused by erroneous spermpicking,as well as by the losing of sperm cell in followed analysis procedure.

大约有20％的管中含有分析样品的精细胞，其余的管是空管。

RESULTS: Some cell colonies were detected at 4 days after pDC316-SOX-9 plasmid transfection. Spindle-shaped cells were collected after clone amplification.

结果：pDC316-SOX-9质粒转染4 d出现小片的细胞集落，挑取克隆扩增培养后，细胞呈梭形纤维状。

An improved CTAB method was used to extract winter buds and tender leaf of ten Species of Spiraea DNA and factors which affect the RAPD amplification were optimized and selected.

试验以10种绣线菊属植物为材料，采用改良CTAB法进行冬芽和嫩叶的总DNA提取并对影响RAPD扩增效果的因素进行优化。

The optimal conditions of the amplification was determined by the square matrix titrating method.

对已知TICD_(50)为10~(3.6)的病毒培养物提取模板以10倍等级倍比稀释，进行PCR扩增，以鉴定PCR方法的敏感性，我们建立的PCR方法所能检测的最小TCID_(50)值为0.398。

That's what makes this mod unique.

这也正是这个MOD独特。

I now feel furious and want to do something.

现在我觉得非常气愤，想做点什么。