查询词典 amplification

Total RNA was isolated from the third instar larval body of the black cutworm, Agrotis ipsilon.The two actin cDNA sequences were cloned by RT-PCR and rapid amplification of cDNA ends and were designated as Aiactin1 and Aiactin2, respectively.

本研究以鳞翅目夜蛾科昆虫小地老虎［Agrotis ipsilon］3龄幼虫整个虫体为材料提取总RNA，利用RT-PCR和cDNA末端快速扩增技术，分别扩增得到2条肌动蛋白的cDNA序列Aiactin1和Aiactin2。

Power for the thermal cycler shall be provided with a manostat or a UPS to avoid the influence on PCR amplification arose by fluctuation of voltage.

热循环仪的电源应专用，并配备一个稳压电源或UPS，以防止由于电压的波动对扩增测定的影响。

Power for the thermal cycler shall be provided with a manostat or a UPS to avoid the influence on PCR amplification arose by fluctuation of volt-age.

热循环仪的电源应专用，并配备一个稳压电源或UPS，以防止由于电压的波动对扩增测定的影响。

The main equipment here is a thermal cycler system for nucleic acid amplification (PCR, quantitative fluorescence detection or common).

主要仪器就是核酸扩增热循环仪（PCR仪，实时荧光或普通的）。

By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法，以Roche试剂盒为标准，比较两公司的DNA聚合酶，用鼠疫菌310 0 4建立荧光PCR反应体系；针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物，检测其灵敏度和特异性，以盲测试验进行验证；在此基础上鉴定2 75株鼠疫耶尔森氏菌，并测试该法检测脏器污染模拟标本的灵敏度。

Based on the predecessor study on deamination of industrial ethylenediamine on normal pressure and on the basic principles of reaction engineering,the method,criteria and parameter of amplification is determined.

根据以乙二胺为原料常压气固相催化合成哌嗪的小试研究结果，依据反应工程学的基本原理，确定了放大方法、放大准则和放大参数。

With forefathers' cognition amplification of nature, the higher requirement is proposed to dietetic deliciousness.

随着先民对自然认知水平的提高，在对饮食的"适口"性方面也逐渐有更高的要求。

The PCR amplification process was 1 cycle initial denaturalization at 94 ℃ for 5 min, followed by 35 cycles, which included denaturalization at 94 ℃ for 30 s, annealing for 1min, extend at 72 ℃ for 2 min.

PCR扩增程序为：94 ℃预变性5 min；94 ℃变性1 min，35 ℃复性1 min，72 ℃延伸90 s，5个循环；94 ℃变性1 min，50 ℃复性1 min，72 ℃延伸90 s，35个循环；72 ℃延伸8 min.4 ℃保存。

The following PCR procedure was selected: 47 circles in the whole amplification: Denaturalize in 94 ℃ for 40s, melt in 36℃ for 50s, extend in 72℃ for 90s.

用下列PCR反应程序：反应过程包括47个循环：94℃变性40s，36℃退火50s，72℃延伸90s。

The lower denaturation temperature can decrease amplification efficiency or even cause the false-negative results of PCR.

较低的变性温度可导致扩增效率降低甚至假阴性的结果。

We are forbidden from giving money to these shoeless, skinny children, as this will perpetuate begging.

向光着脚，骨瘦如柴的孩子施舍是不可以的，因为要招来无休无止的乞讨。

It would be hard not to fall victim to some sharpeyed policeman.

要想不被某个目光锐利的警察抓到，谈何容易。