查询词典 affinity

They probably bind to all proteins, and disrupt some of them, but for some reason their affinity for proteolytic and respiration-related enzymes is particularly obvious.

他们可能绑定到所有的蛋白质，破坏他们中的一些，但出於某种原因，他们的亲和力为蛋白质和呼吸相关酶活性的，特别是显而易见的。

The resin has double effects including absorption and ion exchange; and it simultaneously has hydrophilic basic pyranyl functional groups and hydrophobic styrene frames and has good affinity to amphoteric bioreagents such as the protein.

该树脂具有吸附与离子交换的双重作用。

125I -HPNS-TOC has high affinity to SSTR. Our experiment indicate that 125I-HPNS-TOC is a promising radiotherapeutic for SSTR positive tumors.

125I-HPNS-TOC与SSTR结合力高，有望成为生长抑素受体阳性肿瘤的靶向放射性药物。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

With so many decks violently biased against it, Ravager Affinity probably won't be as successful as it was at German Regionals, but a drop from taking home 40% of the qualification slots hardly means it's no longer the best deck

一名牌手必须考虑以下 2方面的因素来修正矩阵的结果 1：优势的程度 2：比赛中的欢迎程度应该是出现的机会，比如一场比赛套牌特别贵，用的人就少，套牌便宜容易收齐用的人就多，要是为1，2套富人套考虑，结果没出现那叫失算

Object In this study we framed the refusion gene antibacterial peptide[ Cecropin A(l ~ 8)- Magainin (1 - 12),CA(1 ~ 8)-MA(l ~12),CAM] vetor and expressed it in the Escherichia.coli. Thenpurified the recombinded peptide in the Intein Mediated Purificationwith an Affinity Chitin-binding Tag-IMPACT system.

目的 构建重组基因抗菌肽[Cecropin A(1～8)-Magainin(1～12)，CA(1～8)-MA(1～12)，CAM]质粒载体并在大肠杆菌(Escherichia.coli)中表达，应用几丁质自切割分离纯化系统(InteinMediated Purification with an Affinity Chitin-binding Tag，IMPACT)分离纯化抗菌肽，并测定其抗菌活性。

With ultrasonic wave and lysozyme, the recombination N protein is split from bacteria. We dissolve it in guanidine hydrochloride, purify it with Ni〓 affinity chromatography column and renature it in vitro. By testing the antigen of the purified N protein, it shows that its activity is very high.

建立了用超声波和溶菌酶裂解菌体，用盐酸胍溶解，Ni〓亲合层析柱纯化及体外复性等方法，经检测证明纯化的VSV重组核蛋白抗原具有较高的活性。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

The following results have been obtained: 1 Expression of SbNHX1 in E. coli enhanced Na+ and K+ tolerances, but not to others; 2 The sequence from 1353-1514bp in SbNHX1 gene played a role for SbNHX1 to Na+ and K+ tolerances; 3 The Na+/H+ antiporter of Salicornia Bigelovii Torr. catalysed Na+/H+ or K+/H+ exchange with equal affinity, which indicates that the SbNHX1 is more likely to locate on member of vacuolar, and responds to transport Na+ into the vacuole from cytoplasm.

证明了SbNHX1能抵抗Na+和K+的胁迫，但对其它盐离子没有抗性；2）证明了北美海蓬子Na+/H+逆向转运蛋白基因（SbNHX1）C末端从基因的C末端1353bp至1514bp的序列在耐盐中起一定的作用；3）证明了北美海蓬子Na+/H+逆向转运蛋白（SbNHX1）对Na+和K+交换的等亲和性，对于这一点，说明北美海蓬子Na+/H+逆向转运蛋白与定位于液泡膜上的Na+/H+逆向转运蛋白的功能相似。

I go to school at night.

我是上上学。

Clear Tip Nasal Aspirator: A soft time and gentle suction action makes removing excess mucus easy.

鼻吸明确提示：一个柔软的时间和温柔吸力行动使消除过剩的粘液容易。