查询词典 affinity

Objective To evaluate the cytotoxicity and cell affinity of a porcine collagenic dermal matrix to guide the improvement of the porcine dermal scaffold.

目的 通过对一种新型的猪胶原基真皮支架的细胞毒性和与成纤维细胞的亲和性进行评价，为这种新型生物材料的安全应用及工艺改良提供依据。

Methods: Adenosine deaminase was purified by immtino-affinity chromatography, the enzyme activity was assayed colorimetrically by the measurement of ammonia produced over a fixed timeResults: The purified ADA was shown to be a homogeneity by polyacrylamide gel electrophoresis.

用免疫亲和层析法分离纯化人肾ADA，测氨法测定 ADA活性，经SDS-PAGE测定其分子量。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The article put forward the affinity between court that has compellent power and international commercial arbitration directly because arbitration that can settle dispute by non-suit is playing important role.

鉴于仲裁这一非诉讼解决争议的方式在国际商事活动中扮演着日益重要的角色已成为众所周知的事实，论文开门见山即提出法院这一具强制性的国家机关与国际商事仲裁密切且特殊的关系。

The crux and cruces deserts attention in the present creation of short telefilm,such as ,the choice of the visual angle , director assurance to the scene, interview , montage , type and artistic conception, drama conflict, the styles of the course , affinity , sound , short telefilm manner, merger of short telefilm and column, etc.

视角的选择、编导对现场的把握、采访、蒙太奇、典型与意境、戏剧冲突、过程、亲和力、声音、短片的风格、短片与栏目的整合等都是当前短片创作中的难点和关键点。由于短片的创作周期短，加上栏目化生产的一些限制，这些因素经常被编导们忽视，从而导致了短片的艺术性和欣赏性的降低。

Analysis of genetic diversity and affinity relationships among 13 species of Dendrobium Sw.

利用RAPD分析13种石斛属植物的遗传多样性和亲缘关系。

Objective To investigate the removal of endotoxin and bilirubin from ascites of patients with cirrhosis by cellulose diacetate /polyethyleneimine affinity membrane in vitro.

目的研究CA/PEI亲和膜对肝硬化腹水中内毒素及胆红素的去除效果。

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明，成功构建了scFv基因；实现了scFv和scFv在原核中的高效表达，二者的表达量均占菌体总量的35％以上，且成功的纯化了表达的重组蛋白，复性后获得了有活性的纯化重组蛋白。

AIM: To study the effects of over-expression of tricarboxylic acid cycle intermediates transporter NaDC3 (high affinity sodium-dependent dicarboxylate transporter) on energy metabolism and ROS generation in human renal tubular cells.

目的： 研究三羧酸循环中间代谢产物转运蛋白-高亲和力钠依赖性二羧酸转运蛋白(NaDC3)基因过表达对人肾小管细胞能量代谢与活性氧产生的影响。

I go to school at night.

我是上上学。

Clear Tip Nasal Aspirator: A soft time and gentle suction action makes removing excess mucus easy.

鼻吸明确提示：一个柔软的时间和温柔吸力行动使消除过剩的粘液容易。