查询词典 affinity

The usually used electron-transporting materials have disadvantages.They can be easily formed crystal,have lower electron affinity,a narrow gap and difficult to limit the complex exciton to the luminescence layer,all of these disadvantages limit,their application.

有机电致发光材料中常用的电子传输材料容易结晶和低的电子亲和性限制了它的应用；并且能隙较窄，难以将复合激子限制在发光层。

After induction and massive expression of the 3 fusion proteins, the soluble fraction was purified with 50% Ni-NTA resin affinity chromatography, which was proven by SDS-PAGE of the eluate to be a method that can achieve high purity of fusion proteins.

大量诱导表达三种融合蛋白后，取含FA、FB融合蛋白的可溶性组分用50％Ni-NTA树脂过柱纯化，将洗涤部分进行SDS-PAGE检测，表明用此纯化方法能纯化到高纯度的融合蛋白。

Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

After the His·Tag fusion protein was cleaved by the enterokinase, intact biologically active PAT was released from the fusion protein and was purified to homogeneity with Ni2+ affinity chromatography .

用肠激酶切除了融合蛋白的融合部分之后再一次通过金属鏊和层析，经过透析后获得了 PAT 纯品。

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后，所得目的蛋白（IL-29）纯度大于96％，该蛋白N-端序列与理论值一致，其抗病毒活性与IFN-α2b相当。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

However, the Wordsworth epigraph she chose for Silas Marner also highlights the philosophical aspect of her affinity with Wordsworth.

然而，她选择了华兹华斯的题词织工马南传还突出了哲学方面，她的亲和与华兹华斯。

Because fat is poorly perfused, equilibration time is long, especially if the drug has a high affinity for fat.

由于脂肪组织的血液灌注差，达到平衡状态所需的时间也长，特别是那些脂肪亲和力高的药物。

Because fat is poorly perfused, equilibration time is long, e ecially if the drug has a high affinity for fat.

由于脂肪组织的血液灌注差，达到平衡状态所需的时间也长，特别是那些脂肪亲和力高的药物。

METHODS Using a combination of cryoglobulin precipitation,euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography, the impurity protein of different quality was removed, and the easy and effective condition was selected toincrease the purity and content of C2 in the final product.

方法利用冷球蛋白沉淀、优球蛋白沉淀、离子交换色谱、硫酸铵沉淀和亲和色谱几种方法，将血清中不同性质的杂蛋白除去，选择相对简单且分离效果好的条件，提高终产物中目标蛋白C2的纯度和含量。

These are places without aristocratic baggage; egalitarian places open to talent, self-improving, engaged in learning and innovation through networks that were at once competitive and cooperative.

这些地方没有贵族遗风作祟，而且对于那些有天分的人是开放的平等之地，这些人善于通过那些曾经很具有竞争力与合作精神的关系网进行自我提高以及学习创新。

Christine: You don't want to see me?

你不想见我？