查询词典 affinity constant

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中，我们发现，除非过量，否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象；过量的La〓（La〓/CaM摩尔比＞4）将引起钙调蛋白结合功能的下降，同时明显改变金属-钙调蛋白-Mas三元复合物的构象；La〓对Ca〓CaM-Mas的N末端表现高度选择性，显示了在三元复合物中两种离子间的协同效应；在三元体系中，La〓与钙调蛋白的亲和力明显上升，而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异，显示了作用的选择性；Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序，La〓很可能优先与C末端结合，从而使Mas/Mas X首先与C末端结合，该顺序与钙离子相同；La〓的参与使三元复合物在动力学上更加稳定。

Based on the retention and Z (the number of solvent molecules involving in t he displacement from stationary-phase surface ), log I (a constant relating t o the affinity of 1 mol of solute to the bonded stationary phase ), and j (a const ant r elating to the affinity of 1 mol of solvent to the bonded stationary phase ), th e elution strength of acetic acid-water mobile phase was found to be stronger th a n that of methanol-water-TFA.

用参数Z （1 mol溶剂化溶质被溶剂化固定相吸附时从两者接触表面释放出置换剂的摩尔总数），log I （与1 mol溶质对固定相亲和势有关的常数）和j（与1 mol溶剂对固定相亲和势有关的常数）对9种蛋白质在此流动相体系中的保留进行了表征。

Competitive indirect enzyme-linked immunosorbent assay was employed to perform the affinity measurement of dissociation constant and affinity constants for bovine milk lactoferrin and IgY specific against LF using antisera from rabbit and hen as references.

以竞争性ELISA法来测定牛乳乳铁蛋白抗原与蛋黄中抗LF特异性抗体间之解离常数与亲合常数，而同时以兔与鸡之抗血清为对照组。

The immunological traits such as titer,affinity,sensitivity and specificity of this mAb were characterized.The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25∶1.The titer of supernatant hybridoma cell lines of 3F9-C4 was 1∶3.2×10~2 by indirect ELISA.The isotypes in ascites was IgG_(2a)/κ,the affinity constant was 8.4×10~(11)L/mol,IC50 was 8.99μg/L,cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1∶25；筛选出3F9-C4敏感特异的杂交瘤细胞1株，间接ELISA测定细胞培养上清，效价为1∶3.2×102，同种型为IgG2a/κ；腹水的亲和常数为8.4×1011L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

The immunological traits such as titer, affinity, sensitivity and specificity of this mAb were characterized. The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25:1. The titer of supernatant hybridoma cell lines of 3F9-C4 was 1:3.2×10^2 by indirect ELISA. The isotypes in ascites was IgG(subscript 2a)/k, the affinity constant was 8.4×10^11L/mol, IC50 was 8.99μg/L, cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1：25；筛选出3F9-C4敏感特异的杂交瘤细胞l株，间接ELISA 刚定细胞培养上清，效价为l：3.2×10^2，同种型为IgG(下标 2a)/k腹水的亲和常数为8.4×10^11L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

NA, or the amount of contact some of the macro (such as the Faraday constant F, gas constant R, etc.) and micro-volume (such as the elementary charge e, Boltzmann constant k, etc.) of the relationship between a fundamental constant: F = NAe, R = NAk, etc..

NA还是联系某些宏观量（如法拉第常量F，气体常量R等）与微观量（如基本电荷e，玻耳兹曼常量k等）之间关系的一个基本常量：F＝NAe，R＝NAk等。

This design separately uses series linear constant voltage circuit, series regulate constant current circuit and the feedback type contravariant circuit to design direct current constant voltage power supply, direct current constant current power supply and DC-DC converter.

本设计分别用串联型线性稳压电路，串联调整式稳流电路和反馈式逆变电路设计直流稳压电源，直流稳流电源和DC-DC变换器。

A sound style rule bars the instructions of a program from using any numerical or textual constant directly; instead, they rely on symbolic names, and the actual values only appear in a constant definition (constant in Pascal or Ada, preprocessor macros in C, PARAMETER in Fortran 77, constant attributes in the notation of this book).

一条合理的规则禁止把任何数字或本文常数直接地使用在程序的指令中；而是依赖于符号名，并且真实的数值只在一个常数定义中出现Pascal或Ada的常数(constant，C的预处理程序宏， Fortran 77的叁数，在本书符号中的常数属性。

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公司生产销售：颗粒强度测定仪，冷冻干燥机，雪花制冰机，玻璃反应釜，旋转蒸发器，电子天平，超声波清洗机，冰块制冰机，工业制冰机，恒温恒湿箱，显微镜，数显恒温油浴锅/水浴锅，马氟炉，低温冷却液循环泵，循环水式多用真空泵，霉菌培养箱，生化培养箱，恒温培养箱，真空干燥箱，鼓风干燥箱，超净工作台，生物安全柜，磁力搅拌器，离心机，超声波细胞粉碎机，超纯水机，高压灭菌器，实验室各种烧杯，电泳仪电源，实验台，恒温循环器，低温循环高空泵，农残速测仪，紫外分析仪，移液枪，PCR仪，液相色谱，气象色谱仪，酸度计/PH计，分光光度计，超低温冰箱，恒流泵，旋片式真空泵，自动部分收集器，低温恒温槽；等系列产品。

In the first part, we discuss the properties of solutions of (0.1) system with dif-ferent diffusion coefficient and the second boundary condition. The stability of non-negative constant solutions is given, some prior-estimate of the positive steaystateare proved by using the maximum principle and lower-upper solutions, the non-existence of non-constant positive solution, the global exitence of non-constant pos-itive solution and bifurcation solution of non-constant positive steady-states areobtained.

第一章讨论了系统(0.1)在扩散系数不相同的情况下非负常数解的稳定性，运用极值原理，上下解方法确定了系统(0.1)正平衡态解的一些先验估计，然后讨论了非常数解的不存在性，非常数正平衡态解的全局存在性及非常数正平衡态的分歧解。

The quality of both red and white wine depends very much on the wine maker talent and on the quality of the grapes.

他的红酒和白酒的品质，根据酿造者的能力和葡萄的质量，各有差异。

No, I don't want to get drunk before my next match.

" "不，我不想让我的下一场比赛前喝醉了。