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In May 2002 they added Show on drums and then in January 2005 they added Miumi as another vocalist.

在2002年他们加入了新的成员しょう作为鼓手，而后在2005年他们又有新成员みうみ的加入，作为另一个主唱。

The purpose was increasing the body weight and the dead weight of broilers by added the zymosis soybean meal of different proportion in diet.300 AA broilers of one-day-old were divided into three groups randomly.The Ⅰwas control group and the Ⅱ、Ⅲ were experimental group.Ⅰgroup were fed with basic diet andⅡgroup were added with 5% zymosis soybean meal.

将1日龄的300只AA肉鸡随机分成三组，第Ⅰ组为对照组，第Ⅱ、Ⅲ组为实验组，第Ⅰ组饲喂基础日粮，第Ⅱ组的日粮中添加5%的发酵大豆粉，第Ⅲ组的日粮中添加10%的发酵大豆粉，分别在2~6周龄进行体重和屠体重的测定。

All results above account for that CO_2 gas in Jiyang Sag have multi origin and mainly abiogenetic, and mantle is the dominating source, a few may come from biogenesis; and in the process of gas pools forming, some mantle CO_2 lost by converted to CH4 or carbonate, some crust CO_2 added into the gas pools; CO_2 gas pools in Huagou district have particular geochemical character, maybe there were some decarbonated CO_2 added into the gas pools, need further research work.

以上种种特征都说明，济阳坳陷非烃类气藏中气体具有多来源的特征，但CO_2主体是无机成因，并主要来自地幔，少量来自有机成因；在成藏过程，幔源CO_2通过转变为CH_4或者碳酸盐岩而丢失了部分CO_2，壳源CO_2和其它烃类加入到气藏中；花沟地区的气体地球化学特征与众不同，可能存在碳酸盐地层变质脱气产生的CO_2的混入，但具体原因尚待进一步研究。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

The contents of nitrogen, phosphorus and potassium of treatments added pig wastewater were higher than those of treatments added anaerobically digested effluent.

含稻草的堆肥处理，钾的含量明显高于其它处理；添加猪粪水的处理，其氮、磷、钾含量高于添加猪粪水厌氧消化液的处理。

They are aswell blowzy as time passed, added and added humans are application archetypal ugg boots abundant bear.

他们以及blowzy随着时间的推移，增加和补充人类应用原型靴丰富的熊。

Experimental results indicate that the contents of gold from the samples added alkanethiol-derivatised gold are higher then the added chloroauric acid.

实验结果表明含十二烷基硫醇衍生金的样品在90-600℃气相产物带出金含量高于用氯金酸处理过的样品。

The preparation method is characterized in that:(1) at minus 10 DEG C to 10 DEG C, a chloroauric acid solution with a certain concentration is added into a nano-Pt/C catalyst by ultrasonic or stirring mixture and the atomic ratio of Pt to Au is controlled between 18:1 and 5:1;(2) a small amount of monohydric alcohol or dihydric alcohol or trihydric alcohol of C1-C3 is added and is stirred at minus 10 DEG C to 10 DEG C for 10 min to 5h, so as to prepare the carbon-loading Pt-Au bimetallic nano electro-catalyst;(3) if necessary, the catalyst obtained by the step (2) is treated for 0.5 to 4h at high temperature ranging from 100 DEG C to 700 DEG C in the inert or reducing atmosphere, so as to adjust and control the grain size of the Pt-Au bimetallic nano electro-catalyst.

本发明涉及了一种高稳定性碳载Pt-Au双金属纳米电催化剂的制备方法，其特征在于：(1)在-10～10℃中，将一定浓度氯金酸溶液加入到纳米Pt/C催化剂中经超声或搅拌混合，控制Pt∶Au的原子比为18∶1-5∶1；(2)添加少量C 1 -C 3 的一元、二元或三元醇类，在-10～10℃下搅拌10min-5h，制备了碳载铂金双金属纳米电催化剂；(3)视需要，可将步骤(2)得到的催化剂在惰性气氛或还原气氛中经100～700℃高温处理0.5-4h，可调控Pt-Au/C双金属纳米催化剂的粒径。

Mono-cinnamyl maleate was prepared by esterification of maleic anhydride and cinnamyl alcohol of 1 mol:2 mol at 95 ℃ for 2～4 h.Then added isomeric catalyst and mono-cinnamyl fumarate was made by reacting at 90 ℃ for 4 h.After methanol (methanol:mono-cinnamyl fumarate = 6 mol:1 mol) was added,the mixture maintained at 90 ℃ for 4.0 h to synthesize CMF.

合成试验表明：以摩尔比为1：2的马来酸酐与肉桂醇在95 ℃醇解反应2～4 h后加入异构化催化剂，90 ℃转型反应4 h后，添加6倍摩尔质量的甲醇，在90 ℃恒温反应4 h，反丁烯二酸桂醇甲酯的合成产率较高。

MethodThe extracts was prepared by grinding 800-1 000 mg fresh leaves of Clerodendron inerme and Vitex rotundifolia added with liquid nitrogen. The sample of total RNA was prepared by centrifugating the extracts added with reagent at 4 ℃, centrifugating at room temperature after ice bath and centrifugating at room temperature after adding reagent in order.

方法］取800～1 000 mg许树和单叶蔓荆的鲜叶，加液氮研磨后制成提取液，提取液加试剂4 ℃下离心，冰浴后室温下离心，依次添加试剂后室温下离心，即得total RNA样品。

The system can be widely used in belt weighing and batching process control,etc.

介绍了基于智能模糊控制的嵌入式配料系统的设计方法，对模糊PID控制过程和系统调零作了重点的讨论，同时给出了PID控制的程序流程图和系统定长调零子程序。

"I dunno," she answered,"but if you show me aroun', I'll recognize it from the lighthouse just outside the porthole."

"我不知道，"她回答说，"如果你能带我转转，我能认出来的，因为灯塔就在舷窗外。"