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Using a pair of needle nose pliers remove the cotter pin from the upper a-arm's ball joint, save cotter pin for re-installation.

用长嘴钳子从A-ARM的上球头那把固定的PIN取下来，放好，后面需要再装回去

Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

A small baglike attachment forming part of a garment and used to carry small articles, as a flat pouch sewn inside a pair of pants or a piece of material sewn on its sides and bottom to the outside of a shirt.

口袋：一种小型袋状附属物，形成衣物的一部分。被用来携带小的物件，如缝在裤子里的扁平的袋子或缝在衬衣外边的两侧和底部的一片布料 A small sack or bag。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

In her new movie,"The Proposal," Sandra Bullock , playing a Type A witch, totters around in a pair of exquisite high heels, the kind that elongate the legs and give a woman's derri re the gentle backward thrust familiar from fertility figurines.

Sandra Bullock 在她的新片《假结婚》中饰演的角色简直是个 A 型（ A 型行为(一种行为范例或行为模式，其特征是性情急躁，有高度进取心和紧迫感，易引发冠心病)）女魔头，穿着一双秀腿提臀的精致高跟鞋，屁股翘得让人想起了丰满的女性雕像，可惜高跟鞋让她有些摇摇欲坠。

B: Choose a pair of sample containers with bottle lids wrapped in plastics and see the numbers on the sample bottles and lids and pour 50ml of urine from the cup into bottle A, the rest 25ml into bottle B for retest in case bottle A is in doubt.

挑选一套样品容器，看清楚两个样品瓶子和瓶子盖上的号码。从杯中倒50毫升尿样到A瓶中。如果A瓶有问题的话，余下的25毫升倒入B瓶中，供重复实验。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

The result is a complete package - a pair of superb mic preamps feeding an A/D section with a set of standard digital interface outputs, multiple sample-rate choices, and internal or external clock options.

结果是一个完整的软件包-一支优良的麦克风前放一对喂养的A /与标准数字接口输出，D组部分，多采样率的选择，以及内部或外部时钟选择。

We find that the scattering process of the charge and lattice defect is different under different strengths of electric field and the interchain coupling interactions.(1) The results of the dynamics of oppositely charged polarons in polymer chains show that:①At a weak field (.1 mV/A), they finally scatter into an entity, which will be dissociated as a pair of neutral solitons after their first collision and separation.

1聚合物中极化子对的非弹性散射对电场强度的依赖关系表明：在弱场(小于0.1mV/A)时，电荷由于量子隧穿相互抵消，但不改变符号，极化子晶格碰撞后带着剩余的电荷返回，但是它们不能分得很远，在电场作用下，可进行第二次碰撞，结合成一个整体，然后又分成一对中性的孤子对。

The physiology of the skin machine body for the sake of the environment in the maintenance stability, but eject a body metabolism creation of metabolism thing BE'grease', summer perspire many various skin disease light or good, autumn perspire little metabolism thing metabolism don't go out, various skin disease made, this be the skin machine body of various burning disease.

皮肤机体的生理为了维持内环境稳定，而排出身体代谢产生的代谢物是'油泥'，夏天出汗多各种皮肤病就轻或好了，秋天出汗少代谢物代谢不出去，各种皮肤病就犯了，这就是皮肤机体的各种炎症。

If I had anything tender in me, I shot it dead.

如果我有什么招标的话，我枪死亡。