查询词典 Western

The expressed products were purified by Glutathion-Sepnarose chelation affinity chromatography and tested by western blot.

对表达的蛋白进行Western blot分析和谷胱甘肽琼脂糖亲和层析纯化，得到纯化的VP4融合蛋白。

Western Blotting experiments shown that the CspA protein of Bacillus stearothermophilus can be expressed under the condition of not only cold shock but also the induction of Chloromycetin.

用纯化的蛋白质免疫兔子获得抗血清，Western Blotting表明CspA蛋白于嗜热脂肪芽孢杆菌中不仅冷激时表达，而且在受到氯霉素诱导时也会表达。

OBJECTIVE: Western-blot technique is utilized to further validate FHL1 and HOXD13 gene expression in protein levels in muscle tissue of patients with congenital clubfoot, the in vivo HOXD13 and predicted binding sites in embryonic foot development was verified using chromatin immunoprecipitation technology.

目的：应用Western-blot技术进一步验证先天性马蹄内翻足患者足部肌肉组织中FHL1，HOXD13基因蛋白质水平的表达，应用染色质免疫沉淀技术验证在胚胎足部发育时在体内HOXD13和预测结合位点的结合作用。

Using the obtained antiserum as the first antibody, and the monoclonal antibody 4C11 as the collator, the specificity of the antiserum was tested by Western blotting and indirect-ELISA with the Yak recombinant mature PrP, GST fusion protein, the extract of brain and the bacterium protein.

以自制抗血清为一抗，单抗4C11为对照，对牦牛重组成熟朊蛋白、GST蛋白、脑组织提取物和菌体蛋白进行Western blotting检测和间接ELISA试验，验证自制抗血清的特异性。

The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度，并取后极部巩膜组织，用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞，应用免疫组织化学染色和蛋白印迹（Western-blot）法检测bFGF和bcl-2蛋白。

Results:Western blotting analysis showed that the product had apparent cross reaction with huMCP-1 monoclonal antibody.

结果：Western blot证明与huMCP-1单克隆抗体有明显交叉反应。

Third, the prepared McAbs were identified by cytochemistry, specificity and Western-blot.

然后用细胞化学、特异性、Western-blot等方法鉴定制备抗体的免疫反应及特异性。

Western blotting showed less expression of cytochrome c in Hsp90 overexpression system than in control.

Western blotting检测Hsp90细胞中细胞色素c的表达与对照组相比明显减少。

Methods: We used virus proliferation assay cell viability assay to evaluate the proliferation and cytolysis selectivity of CNHK500. And we used Western-blot to confirm the expression of adenovirus CNHK500 E1A and E1B in cancer and normal cells.

行病毒增殖实验和细胞生长抑制实验，验证CNHK500选择性复制和杀伤能力；Western blot检测腺病毒E1A和E1B在细胞中的表达。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。