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Methods Recombinant adenovirus GDF-5 (Ad-GDF-5) containing green fluorescent protein and Ad-GFP were amplifiedand tittered. hBMSCs at passage 3 were infected with two viruses at different titers. At 2 days after intervention, GFP expression was observed using fluorescence microscope, and GDF-5 expression in hBMSCs was detected by RT-PCR.

将重组腺病毒GDF-5（adenovirus GDF-5，Ad-GDF-5）和空载腺病毒Ad-GFP 进行扩增并测定滴度，并以不同滴度两种病毒感染第3 代hBMSCs，干预后2 d 荧光显微镜下观察GFP 表达情况，RT-PCR 检测GDF-5 在hBMSCs中的表达。

Methods: We transplanted neural stem cells from embryobrain of rats in hippocampi of AD model rats transecting fimbria fornix with or without FG. 30 days after transplanting, test these AD models' behavior then inspect living and differentiation of NSCs by immuno -fluorescence.

体外分离培养大鼠胚脑神经干细胞，用纤维蛋白凝胶为支架移植入穹隆海马伞切断AD模型鼠海马，行为学测试后用免疫组织化学方法检测神经干细胞存活和分化情况。

" It is a new term that is eventually expected to replace AD AD is an acronym for " Anno Domini " in Latin or " the year of the Lord in English.

&这是一个新的术语，它最终将取代广告广告是多米尼缩写为&安诺&在拉丁美洲或&主一年的英语的研究。

AD 或 BC AD=Anno Domini 公元,BC=Before Christ

公元前。不带点的公元或公元前

Banner categories:: Production Design banner ads tower lights flag flag Rome carrying the banner of the election campaign banner flag of the flag Xiaoqi classes full-color flag embroidered cloth flag upright cloth banner ads Screen Printing Industry flag flag flag embroidered banner of computer embroidery trademark knitting Trademark knitting trademark / texture superscript LOGO manufacturing industrial and commercial advertising flag lights flag religious flag Commerce and Industry elections straps shoulder strap flag ads cloth vest activities shoulder strap T-shirt label States flag embroidered cloth flag of the vertical banner ad flag flag Screen Printing Industry Flag trademark embroidered banner of uniformed flag Jiaoqiduiqi Cross Societies flag banner of cloth banner of San Jiaoqi string flag banner printing plant produced the banner campaign banner banner flag at the company store banner ad banner banner design manufacturing.

旗帜类：：旗帜制作设计广告直立旗路灯旗罗马旗帜选举旗帜竞选手拿旗纪念旗校旗班旗全彩布条刺绣直立旗布条广告旗工商旗网印旗会旗商标刺绣旗帜电脑刺绣针织商标针织商标/织标LOGO专业制造工商广告旗路灯旗宗教旗选举背带工商肩带会旗广告布条背心活动T恤帽子肩带各国国旗刺绣旗帜直立旗布条广告旗工商旗网印旗商标刺绣旗帜军警旗校旗队旗社团旗旗帜横布条三角旗串旗旗帜印刷旗帜制作旗帜厂竞选旗帜旗帜店旗帜公司旗座广告旗帜设计旗帜制造。

Gilpin, in his account of the forest borderers of England, says that "the encroachments of trespassers, and the houses and fences thus raised on the borders of the forest," were "considered as great nuisances by the old forest law, and were severely punished under the name of purprestures, as tending ad terrorem ferarum —— ad nocumentum forestae, etc.," to the frightening of the game and the detriment of the forest.

吉尔平在他的英格兰森林中的居民记录里面，写着：&一些人侵占了土地，在森林中就这样筑了篱笆，造了屋子，&在&古老的森林法规中，这是被认为很有害的而要以强占土地的罪名重罚的，因为ad terrorem ferarum——ad nocumentum fore-stae等等&使飞禽恐惧，使森林受损。

The kernel of thecontrol system hardware is an industrial control PC, voltage AD, current AD anddigital input/output plates are equipped to respective signals collected, which collectand control main parameters of transmission assembly. For the convenience of wiringand signal modulating, a harness plate adapting to collection plate is configured.

测控系统的硬件以一台工业控制PC机为核心并根据采集信号的性质配置了电压-数字量转换板、电流-数字量转换板及数字量输入/输出板，对变速箱总成的主要参数进行采集和输入转速等的控制；为方便接线和信号调理，配备了与采集板对应的接线和调理接口板以及接线盒。

In 1009 AD, the Fatimite Khalif of Egypt al-Hakim explicitly ordered the destruction of the Church of the Holy Sepulchre. The Arab historian Yahia ibn Sa'id describe the events thus:"the holy deed commenced on Tuesday, the fifth day before the end of the month of Safar of the year 400 of the Egira (1009 AD in our calendar). Only those parts of difficult access were spared".

西元1009年埃及的明确下令摧毁圣墓大殿，阿拉伯的史学家描述了此件事件：&这神圣举动开始於星期二，直到Egira 400年撒法月底(即西元1009年)前五天结束，只有殿堂内不易为人所到之地才此灾殃。&

She glibly ad -libbed anything that popped into her he ad .

她从容不迫地穿插了她偶然想到的话。

Toles depicts Kerry and an ad visor strolling past the White House, atop which workmen are busy covering a billboard with an anti-Kerry ad

在托尔斯的漫画中，克里和一位幕僚正漫步走过白宫，而在白宫顶上两个工人正忙着画完一个写有反对克里标语的宣传牌。

Yet, Edith says, there was always money in the bank.

但是Edith说，她的银行户口里面总是有钱。

We have created a series of files that resolve this problem--one for each day of the 40 days of purpose.

我们制作一系列的档案去解决这问题---一个为标竿40天中的每一天。