培养基

词组短语

culture medium · culture media · culture-medium

与培养基相关的网络例句 [注：此内容来源于网络，仅供参考]

With the corm and scale of A. albus in Zigui County in Hubei Province as tested material, MS was used as the basic medium with different hormones to respectively match 10 kinds of media for induction, differentiation for tissue culture of A. albus The induced clumpy buds were cut into single plants and were put into the rooting medium of MS+NAA 0.1-0.5 mg/L and the effects of different hormone matching on callus formation, bud differentiation and rooting were studied.

方法］以湖北省秭归县的花魔芋球茎和鳞片作为外植体，以MS培养基为基本培养基，添加不同激素，分别组配成10种诱导培养基和分化培养基进行组织培养，然后将诱导的魔芋丛芽切成单株后接入MS+NAA0.1~0.5 mg/L生根培养基，研究不同激素配比对愈伤组织形成和芽分化以及生根的影响。

It indicated that the growth and acid production of S.mutans were higer in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while lower in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration.

在8%、12%、16%浓度下，赤藓糖醇培养基的A值较木糖醇培养基低，pH值较木糖醇培养基高，说明变异链球菌在含8%、12%、16%赤藓糖醇的培养基内的生长和产酸能力明显较相同浓度木糖醇培养基低。

Results The data of A were higher in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while lower in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration. The data of pH were lower in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while higer in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration.

结果 在0.5%、1%、2%浓度下，赤藓糖醇培养基的A值较木糖醇培养基高，pH值较木糖醇培养基低，说明变异链球菌在含0.5%、1%、2%赤藓糖醇的培养基内的生长和产酸能力明显较相同浓度木糖醇培养基高。

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

This paper presents a study of Eucalyptus dunnii tissue culture, using its seeds as explants. The results reveal that MS medium is a suitable basic medium for its seeds to germinate and grow; that MS+KT1.0 mg/L+2, 4-D2.0 mg/L+Homocysteine30mg/L is a good medium to induce its seeds to dedifferentiate directly into callus; that H+6-BA2.0 mg/L+IAA0.2 mg/L is a good medium to make its bud on the seedling reproduce more buds; that MS+6-BA2.0 mg/L+NAA0.2 mg/L is a better medium to induce its root on the seedling into callus; that B5+6-BA2.0 mg/L+2, 4-D0.2 mg/L can induce its under-hypocotyl to differentiate into buds; and that B5+Ad2.0 mg/L+IAA0.2 mg/L can induce its under-hypocotyl into callus to generate normal buds.

以邓恩桉种子为外植体，探讨用最少种子快速繁殖最多幼苗的方法，结果表明：MS培养基是较适合邓恩桉种子萌芽和生长的基本培养基；诱导种子直接脱分化成愈伤组织的较佳培养基配方为MS+KT1.0 mg/L+2，4-D2.0 mg/L+半胱氨酸30 mg/L；以邓恩桉实生苗的芽来繁芽的较理想培养基配方为H+BA2.0 mg/L+IAA0.2 mg/L；邓恩桉实生苗的根诱导愈伤组织的较佳培养基配方为MS+6-BA2.0 mg/L+NAA0.2 mg/L；诱导邓恩桉下胚轴分化芽较佳培养基配方为B5+6-BA2.0 mg/L+2，4-D0.2 mg/L；诱导邓恩桉下胚轴脱分化为胚性愈伤组织的较佳培养基配方为B5+Ad2.0 mg/L+IAA0.2 mg/L。

A pectinase from CXJZ95-198 was purified. Results have made know:(1) The changes of protein content secreted by CXJZ95-198 in the five media glucose mannose xylan mannosan and pectin were 1.28mg/ml, 1.17mg/ml, 0.94mg/ml, 0.75mg/ml and 0.73mg/ml.The pectinase activity was maximum in the mannose (79.39U/ml) with the order of glucose(73.33 U/ml) mannosan(24.25 U/ml) xylan(17.45 U/ml) and pectin(15.98 U/ml).

在本研究条件下，得到如下结论：(1) CXJZ95-198菌株在以葡萄糖作为碳源的培养基中蛋白质含量变化最大，达到了1.28mg/ml，其次依次为甘露糖、木聚糖、魔芋粉和果胶培养基，分别为1.17 mg/ml，0.94 mg/ml，0.75 mg/ml，0.73 mg/ml；在这五种培养基中，以甘露糖培养基中果胶酶活性最高，达到了79.39 U/ml，其次依次为葡萄糖培养基，魔芋粉培养基，木聚糖培养基，最后为果胶培养基，分别为73.33 U/ml，24.25 U/ml，17.45 U/ml，15.98 U/ml。

The growth of plantlets with the height of 1-2 cm and 3-4 leaves was compared on MS basal medium containing various organic compounds, including banana, carrot, potato, coconut water, and tryptone. The result showed that the growth of oncidium plantlets was the best on medium containing banana, potato and tryptone altogether with respect to their plant height, leave number, root number, root length, fresh weight, and dry weight. The next better media were the control and the Banana medium, followed by the Potato medium.

另外取高约1-2公分，带3-4片叶片的分化小苗，培养於含不同浓度有机添加物（香蕉、胡萝卜、马铃薯、椰子水、及tryptone）的半量MS培养基，以探讨最适合文心兰瓶苗生长的培养基，试验结果显示，在同时含香蕉、马铃薯及tryptone三种成份的培养基生长的小植株不论在株高、叶数、根数、根长、鲜重、及乾重均较其他培养基为佳；不含添加物之1/2MS培养基、及含香蕉的培养基次之，而在含马铃薯之培养基较差。

Additionally, the growth of the pathogen was obviously affected by cultural media. The morphological characters such as colony color and diameter, and production of fruit body of the fungus growing on PDA, 10% V8, apple leaves dextrose agar and apple leaves extraction dextrose agar media were different. The fungus formed colonies of about 8mm in diameter and did not produce fruit bodies and aerial hyphae in 1 month incubation on PDA. However it formed the similar size colonies as on PDA and produced fruit bodies and aerial hyphae on 10% V8 and LDA media in the same incubation period. Very small colonies (2mm in diameter) and fruit bodies were found on LEDA media in the same incubation condition. These results indicated that successful isolation of M. coronaria from apple leaves depended on suitable isolation method and cultural media as well as fresh samples.

不同培养基上菌落形态、大小和产孢情况差异也很大，培养1个月（25℃）后PDA上菌落黑褐色隆起，表面蚯蚓粪状，无气生菌丝，无子实体和基内菌丝；10% V8培养基上菌落中央隆起，黑褐色，表面生少量气生菌丝，边缘放射状，基内菌丝深褐色，有子实体；苹果叶片葡萄糖琼脂培养基上菌落平坦，黄褐色，表面生茂密的金黄色气生菌丝，基内菌丝深褐色，有子实体；苹果叶片煎汁葡萄糖琼脂培养基上菌落有明显的不规则隆起，黄褐色至黑褐色，表面生少许气生菌丝，菌落生长缓慢，无基内菌丝，分生孢子盘菌落表面生，菌落直径仅2mm左右，而在其他培养基上的菌落直径可达6－8mm，说明培养基质、分离方法均对苹果盘二孢的分离培养和生长发育有明显的影响。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

At the same time, the study found an easy and simple resistant identification method in seedling stage and carried on resistant identification of Verticillium dahiea wilt to tomato germplasm resources that are kept by our lab.

生物学特性研究表明，适于番茄黄萎病菌生长的培养基为PSA培养基，低量蔗糖酵母培养基，马铃薯胡萝卜培养基及蔡氏培养基；在马铃薯胡萝卜培养基上产孢效果最理想。

与培养基相关的网络解释 [注：此内容来源于网络，仅供参考]

Malt Agar：麦芽什琼脂,麦芽洋菜培养基,麦牙洋菜培养基,(培养基)麦芽琼脂

malt adjuncts 麦芽添加物 | malt agar 麦芽什琼脂,麦芽洋菜培养基,麦牙洋菜培养基,(培养基)麦芽琼脂 | malt albumen 麦芽蛋白

Anaerobic medium：厌氧培养基

培养基按营养组成和用途,分为基础培养基(basic medium)、增菌培养基(enrichment medium)、鉴别培养基(differential medium)、选择培养基(selective medium)和厌氧培养基(anaerobic medium)等;按物理性状分为液体培养基、半固体培养基和固体

culture medium：培养基

培养基(Culture medium)是从无土栽培的营养液发展而来的,在某种意义上说就是模拟土壤. 最初的培养基就是简单的马铃薯浸出液即土豆汁. 后来随着植物生理学和生物化学的研究的深入,培养基越来越复杂,成分越来越多.

defined medium：组合培养基

培养基的种类及其应用按对培养基的了解来分天然培养基(complex medium, undefined medium),较经济,一般用于大规模生产组合培养基(defined medium)或称合成培养基(synthetic medium),较昂贵,一般用于研究工作(代谢、遗传分析),特点:组份精确,

defined medium：确定成分培养基

基础培养基 basic medium | 确定成分培养基 defined medium | 合成培养基 synthetic medium

dehydrated medium：干燥培养基;脱水培养基

culture medium(微生物)培养基 | dehydrated medium干燥培养基;脱水培养基 | elective medium选择培养基

enrichment medium：富集培养基

3)加富培养基和富集培养基(enrichment medium):在普通培养基(如肉汤蛋白胨培养基)中加入某些特殊营养物质制成的一类营养丰富的培养基. 这些特殊营养物质包括血液、血清、酵母浸膏、动植物组织液等,用来培养营养要求比较苛刻的异养型微生物,

enrichment medium：增菌培养基

培养基按营养组成和用途,分为基础培养基(basic medium)、增菌培养基(enrichment medium)、鉴别培养基(differential medium)、选择培养基(selective medium)和厌氧培养基(anaerobic medium)等;按物理性状分为液体培养基、半固体培养基和固体培养基三大类.

enrichment medium：富集培养基,增菌培养基

enrichment 富集 | enrichment medium 富集培养基,增菌培养基 | ensemble effect 集团效应

semisolid medium：半固体培养基

29.半固体培养基(semisolid medium) 在液态培养基中加入凝固剂的量比固体培养基中的少而制成的半固体状态的培养基. 31.基础培养基(minimum medium) 含有一般微生物生长所需基本营养物质的培养基. 32.加富培养基(enrichment medium) 在基础培养基中加入某些特殊营养物质,