非活性的

Purification and analysis of AT-toxin Firstly, we detected the components of protein and nonprotein, the results showed that AT-toxin is a nonprotein. We have carried out the liquid chromatogram analysis of the toxin. The result indicates that there are many components in this toxin and there are four kinds of components which content is more than others. At last, we have the experiment of separating the cpmpositions with the silica gel and we get four components which are active.

AT-毒素的初步纯化与分析为确定AT-毒素的组分，首先对毒素的蛋白质和非蛋白质组分的毒性进行了测定，结果表明：AT-毒素活性组分为非蛋白质；对毒素进行硅胶G薄层层析，初步分离得到10种组分，其中四种组分具有活性：组分Ⅵ、Ⅶ、Ⅷ、Ⅸ，初步说明了该毒素含有多种组分；进一步对该毒素进行液相色谱分析，也发现该毒素含多种组分，其中四种组分峰高和峰面积较大，含量较高；其致毒活性可能是由这些组分协同作用的结果，它们之间可能存在正协同或负协同作用关系。

We employed HPLC to measure the metabolites of caffeine in the whole blood and calculated the ratio be between the metabolite and caffeine, which was used as index to evaluate the effect of Qingkailing injection on rat CYP1A2 activity in vivo ; We also detected the CYP1A2 and CYP2D6 activity in microsomal reconstituted system by analysis of phenacetin metabolism and dextromethorphan metabolism with HPLC.

通过HPLC法测定全血中咖啡因的代谢率，观测清开灵注射液对大鼠CYP1A2活性的影响；通过HPLC法测定大鼠肝微粒体重组系统非那西丁的代谢比率，确定清开灵注射液对大鼠肝微粒体CYP1A2亚型的作用；测定大鼠肝微粒体重组系统右美沙芬的代谢比率，确定清开灵注射液对大鼠肝微粒体CYP2D6亚型的作用。

Results: Caspase-3 mRNA was constitutively expressed at a low level in freshlyisolated CD34+ cells. The expression of caspase-3 mRNA and protein was upregulated when these cellswere first expanded in suspension culture with growth factors for 3 days. However, only the 32 kDa inactivecaspase-3 proenzyme was detected in the freshly isolated CD34+ cells as well as during the first 3 daysexpansion with cytokines.

检测结果显示，Caspase-3mRNA在新鲜分离的脐血CD34+细胞中低水平表达，在细胞因子支持下体外培养3天，扩增的CD34+细胞中Caspase-3mRNA和蛋白质水平表达上调，但在这两种细胞中仅能检测到分子量为32KDa的非活性酶原形式的Caspase-3；随着体外培养时间的延长，在IL-3，IL-6和GM-CSF组合条件下，Caspase-3被激活，可检测到分子量为20KDa的裂解片段。

From the calculations, it can be found that the van der Waals interactions, the hydrophobic interactions, as well as the H-bonding interactions are crucial for the ligand binding. The 4-phenylamino group can produce strong van der Waals adn hydrophobic interactions with the nonpolar side chains of the residues deep in the binding cleft. The R^1 and R^2 substituents on the bicyclic chromophore can also produce strong van der Waals and hydrophobic interactions with the residues located at the exterior part of the binding pocket. Moreover, the two N atoms of the quinazoline can form H-bonds with EGFR, which will produce significant contribution to biological activities. The calculated nonbonded interactions between anilinoquinazolines and EGFR, as well as the information obtained from the predicted complexes, can interpret the structure-activities of the inhibitors well, which can afford us important information for structure-based drug design.

从模拟结果得到的抑制剂和靶酶之间的相互作用模式表明范德华相互作用、疏水相互作用以及氢键相互作用对抑制剂的活性都有重要的影响，抑制剂的苯胺部分位于活性口袋的底部，能够与受体残基的非极性侧链产生很强的范德华和疏水相互作用，抑制剂双环上的取代基团也能和活性口袋外部的部分残基形成一定的范德华和疏水性相互作用，而抑制剂喹唑啉环上的氮原子能和周围的残基形成较强的氢键相互作用，对抑制剂的活性有较大的影响，计算得到抑制剂和靶酶之间的非键相互作用能以及抑制剂和靶酶之间的相互作用信息能够很好地解释抑制剂活性和结构的关系，为全新抑制剂的设计提供了重要的结构信息。

The aim of this contribution is to draw a general perspective of the liveness and safeness for Asymmetric Choice nets.

文章讨论了活性具有单调性的非对称选择网活性与安全性的条件，并给出一个多项式时间算法来判定一个给定的非对称选择网是否是活的、安全的与活性满足单调性。

Three kinds of CpTiCl had been synthesized when was catechol , 2, 2′-biphenol , or 2, 2′-binaphthol , respectively. In the case of CpTiCl〓, was 8-hydroxylquinoline , 2-methyl-8-hydroxyl-quinoline , and 8hydroxyl-5-nitro-quinoline , respectively.

研究表明，这三类半茂钛配合物在助催化剂MAO作用下，于30℃、0.1MPa乙烯压力下均可催化乙烯聚合，并显示较高的活性，远高于未引入非茂配体的CpTiCl〓及不含茂环的相应非茂配合物的活性，这说明同时含一个茂配体和一个非茂配体的半茂配合物是烯烃聚合的优良催化剂。

O PSII supercomplex was formed in the plant grown in iron depletion solution. After treatment with NO, all of the above injuries of plant due to iron deficiency were reverted, such as the increase of active reaction center of PSII, the reconnection of PSII with antenna, the increased electron transport rate, the increased photochemical fluorescence quenching and the reduced non-photochemical fluorescence quenching.

O处理后，植物受到的所有缺铁损伤都得到恢复，有活性的PSII反应中心数量增加，与天线的连接恢复完好，电子传递效率增高，同时叶绿素荧光的光化学淬灭增多，非光化学淬灭降低，表明PSII下游的电子传递体也得到很好的修复。

Nickel phosphide was chosen as the HDN catalyst in the present dissertation because it is reported to have the highest activity among transition metal phosphides. For the supported nickel phosphide catalysts, mesoporous MCM-41 was used as the carrier. The effect of support modification and the introduction of a secondary metal species on the activity of supported nickel phosphide catalysts were studied.

本论文以磷化镍催化剂为研究对象，考察了MCM-41负载的磷化镍催化剂的制备及其对喹啉的HDN反应性能，载体和活性组分的改性对喹啉HDN反应性能的影响，以及TiO_2的引入对非负载的磷化镍催化剂HDN反应的影响。

Cellular fibronectin is a kind of non-collagen glucoprotein. It is present mostly in periodontium, taking part in many physiological functions, such as cell junction, proliferation and differentiation, reparation and arrangement of epithelial tissue, immunoregulation of organism.

细胞纤连蛋白是一种具有生物学活性的非胶原糖蛋白，大量的存在于人的牙周组织中，排列规律，参与机体细胞的连接黏附、增殖分化和上皮组织的修复以及机体免疫调控等多种生理活动，是维持牙周附着的主要物质。

Fibronectin is a very important biological molecule in collagen glucoprotein that plays a number of important roles in the extracellular matrix. It has roles in structural support and signaling cues for cell survival, migration, differentiation, gene expression, growth factor signaling, cell contractility, epithelial tissue repair, alignment regularization and organism immunoregulation.

纤维连接蛋白(fibronectin，FN)是一种具有重要生物学活性的非胶原糖蛋白，是细胞外基质(extracellular matrix，ECM)的重要组成部分，参与机体的细胞连接黏附、增殖分化、信号传导、基因表达、收缩调节、上皮组织修复、排列规则化以及机体免疫调控等重要的生理功能。

Further more, the simplified analysis method on stressed-skin effect of corrugated steel sheet is also discussed in detail.

此外还比较详细地探讨了蒙皮效应的简化分析方法。

If I had known her telephone number,I would have called her.

要是我知道她的电话号码，我就打电话给她了。