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In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya,以绿色荧光蛋白基因为报告基因,构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46,荧光显微镜检测发现,此启动子在两菌株中都具有功能。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒,带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子,载体大小9880bp。

That in female was Uu, Ng, Mh, eumycetes, Trichomonas and Ct in descending sequence. Constituent ratio of eumycetes and Trichomonas in female was higher than that in male (x^2=50.0l, P.001), while that of NG was higher in male (x^2=13.86, P.01), and mixed infection rate was higher in female (x^2=6.98, P.05). Conclusion Ng and Uu were the common pathogens of genitourinary infection in male, while eumycetes and Uu were the common pathogens in female.

结果 125例男性病人病原体感染构成比由高到低依次为Uu、Ng、Mh、霉菌、Ct和滴虫;l96例女性病人病原体感染构成比由高到低依次为Uu、Ng、Mh、霉菌、滴虫和Ct,男女感染病原体比较,女性霉菌和滴虫的感染构成比高于男性(x^2=50.01, P.00l),男性淋球菌感染构成比高于女性(x^2=1386,p.01),混合感染率女性高于男性(x^2=6.98,P.05)。

In this study,we investigate the efficacy and safety of intraocular use of fluconazole in the treatment of ocular fungal infection. Ten patients with intraocular fungal infections were examined. Among these patients , eight were infected with keratomycosis with intraocular spreading, one had postoperative fungal endophthalmitis after cataract operation with an intraocular lens implant, and another suffered from endogenous fungal endophthalmitis.

为探讨眼球内注射fluconazole用於治疗眼球内霉菌感染之效果以及安全性;我们搜集十位眼球内霉菌感染的病人,其中包括八位严重角膜霉菌感染;两位为霉性眼内炎,这些病人在传统治疗无法有效控制感染后,都曾接受眼球内注射fluconazole的治疗,包括前房灌洗、前房内注射或玻璃体内注射,使用浓度为5-10g/ml。

The results showed that human demodex (including demodex folliculorum and demodex brevis) and fungus including Aspergillus, Penicillium, Microsporum of the dermatophytes, Candida and Mucor, ect.

其中人体蠕形螨包括毛囊蠕形螨和皮脂蠕形螨,真菌包括曲霉菌、青霉菌、皮肤丝状菌的小孢子菌、念珠菌和毛霉菌等。

Mucormycosis is an acute fatal infection caused by a fungus of the order Mucorales.

霉菌病是一种由白霉菌目所造成的急性致命的霉菌感染。

The rest of the word are subject to various fungi, Mycotoxin is the secondary metabolism of toxic products in the course of certain fungal growth and reproduction.

摘 要:霉菌遍布世界各地,种类繁多。霉菌毒素是某些霉菌在生长繁殖过程中产生的二次代谢有毒产物。

The results showed that Phytophthora sojae on the cellophane carrot agar could not produce oospores within 10 days, no medium were attached when Phytophthora sojae was taken away from cellophane, high-quality RNA, which could meet the requirement of differential display reverse transcriptional PCR was obtained, indicating that it was a suitable cultivation method for Phytophthora sojae to produce pure vegetative mycelia.

结果表明,玻璃纸胡萝卜琼脂培养的大豆疫霉菌,10 d内不会产生卵孢子,当大豆疫霉菌从玻璃纸上取下时,不会带有培养基,能够提取出高质量的RNA,满足差异显示反转录PCR对RNA质量的要求,是一种适合大豆疫霉菌纯营养菌丝的培养方法。

Culture medium, water volume, root exudates of different crops, temperature, illumination affecting germination conditions of oospores of Phytophthora sojae studied in this reasch and compared the difference of germination of oospores of different race of Phytophthora sojae, filtrated the higher germination rate of strain and suitbable conditions combination of germination of oospores; The pure-line(Race 1) in virulence was obtained through isolation and culture and virulence identify of the second generation. The pure-line was analyzed by molecule method of ISSR. The results were summarized as follows:There was quite difference of germination rate of oospores on different culture medium.

本文测定了不同培养基、水量、农作物根渗出液、温度、光照对大豆疫霉菌卵孢子萌发影响,比较了大豆疫霉菌株间卵孢子萌发的差异,筛选出萌发率较高的菌株和适于卵孢子萌发的条什组合;通过继代单卵孢株分离培养和毒性鉴定,建立了大豆疫霉菌单卵孢株毒性纯系,并分析了单卵孢株毒性纯系后代群体ISSR分子指纹,结果如下: 1。

Just started a three-day fever, the highest 39.1 degrees, was later linked to the hospital for three days of water, the fourth day of diarrhea and vomiting since the afternoon, go to the hospital linked to water, hanging by nearly a week见好not actually check the stool also found fungal infection, immediately after the cessation of linked water, to do massage with the enema, it is not diarrhea, but is still very hard stools, a one, may I ask how can we rule fungal infection?

宝宝前阵子拉肚子,挂了好几天的水,抗生素用多了,霉菌感染怎么办?刚开始三天发烧,最高39.1度,后来去医院挂了三天的水,第四天下午开始腹泻呕吐,去医院挂水,挂了近一个礼拜都不见好,居然还检查大便后发现霉菌感染,后马上停止挂水,改做推拿跟灌肠,现不腹泻,但大便还是很硬,一粒一粒,请问怎么才能治霉菌感染?

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