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The sequence analysis of ITS region could provide theory basis for the identification of Alternaria Nees.

结论]ITS序列分析法可为链格孢属的分子鉴定提供理论依据。

The study aimed to identify Alternaria Nees from some areas of China at molecular level by analyzing the rDNA ITS sequence.

[目的]对我国部分区域链格孢属rDNA ITS序列进行分析,从而在分子水平上对其进行鉴定。

Alternaria is a dematiaceous mitosporic fungus with a wide variety of habitats and geographical distribution.

链格孢是半知菌暗色丝孢菌中重要的一类真菌,该属真菌种类多,寄主范围和地理分布广泛。95%以上的种能兼性寄生在植物上,是重要的植物病原菌。

Therefore, Alternaria is the main pathogen of cotton leaf spot. 2. Pathogenicity test of the above-mentioned 26 strains showed that they are the cotton leaf spot pathogens as the eumycetes isolated from the Invasived plants are the same with the pathogenetic strains.

根据科赫氏法则从接种发病植株叶片上分离病菌,其形态特征与从自然发病植株上分离的相同,证实这些菌株就是棉花叶斑病的致病菌,其中链格孢属是棉花叶斑病的主要致病菌属 3。

In the four ecosystem types, species of Alternaria and Ulocladium had wider niche breadth and were of widely adaptive species, while species of Bipolaris, Cirrenaria, Curvularia, Doratomyces, Graphium, Myrothecium, Scolecobasidium, Scopulariopsis, Scytalidium and Stachybotrys had narrower niche breadth and were of narrowly adaptive species.

在上述四类生境中,链格孢属Alternaria和细基格孢属Ulocladium真菌具有较宽的生态位,为广适性物种;而平脐蠕孢属Bipolaris、卷旋孢属Cirrenaria、弯孢属Curvularia、矛束霉属Doratomyces、粘束孢属Graphium、漆斑霉属Myrothecium、齿梗孢属Scolecobasidium、帚霉属Scopulariopsis、节隔孢属Scytalidium和葡萄穂霉属Stachybotrys10个属真菌的生态位较窄,为狭适性物种。

Atopy was defined as a positive skin-prick test to mites, cat, Alternaria, Cladosporium, grass, birch, Parietaria, olive, or ragweed.

特应性定义为对螨类、链格孢属、分子孢子菌属、草、桦木、墙草属、橄榄或豚草属皮肤划痕实验阳性。

Tobacco brown spot caused by Alternaria alternataKeissler is an important fungal disease on the crop worldwide and makes large economic losses in tobacco maturation period.

烟草赤星病是烟草生产上重要的真菌性病害,其病原菌为链格孢属真菌,在烟草成熟后期危害十分严重,造成重大经济损失。

Tobacco brown spot disease has been an important leaf disease in tobacco. In tobacco maturation period, this disease causes tobacco brown spot disease seriously, and makes large economic losses. Its main pathogen is Alternaria alterrrata Keisslar.

烟草赤星病是烟草的一类重要叶部病害,其主要病原菌为链格孢属真菌Alternaria alterrrata (Fties Keisslar,在烟草成熟后期危害十分严重,造成重大经济损失。

After pathogenic fungi identified,we found 26 of them can cause cotton leaf spot. The 26 pathogenetic strains were carried out morphological and molecular identification of the firm. The initial identification of the pathogens is through the morphological observation such as the determination of growth, colony morphology, conidia and conidial stalk.

通过对生长量的测定,菌落形态,分生孢子和分生孢子梗等形态学观察进行形态学鉴定;通过PCR扩增真菌核糖体基因转录间隔区序列并测序,并将序列在GenBank上进行同源性比对和构建系统系统发育树等方法进行分子鉴定,鉴定出这26株真菌分属链格孢属、镰孢属、黑孢属、刺盘孢属、匍柄霉属等5个属。

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