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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

It was—simply amazing," she repeated abstractedly."But I swore I wouldn't tell it and here I am tantalizing you.

这事……太惊人了,"她出神地重复说,"可是我发过誓不告诉别人,而我现在已经在逗你了。

Delivery room accoucheuse cannot repeat mechanically " not impatient bearing a dot, do not be fond of no less than unripe coming " and ignore a patient experience, should go up in spirit, support gives on physical strength, make the puerpera is in the process that faces anguish, do not feel loneliness is mixed helpless, make its acquire contented feeling and safe move in mentally, experience comfortable reach the warmth like the family member. 2 increase love, patience, guidance applies an abdomen to press palace mouth to leave complete hind, the puerpera is advanced delivery room, because palace shrinks painful reach an environment to change and cause mental tension, produce scared psychology, need human nature to change more right now nurse.

产房助产士不能机械地重复"别性急忍着点,不疼生不下来"而忽视病人的感受,要在精神上、体力上给予支持,使产妇在面对痛苦的过程中,不感到孤独和无助,使其在心理上获得满足感和平安感,感受到舒适及亲人般的暖和。2增加爱心、耐心,指导运用腹压宫口开全后,产妇被推进产房,由于宫缩痛及环境改变而引起精神紧张,产生惧怕心理,此时更需要人性化护理。

The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps-: a coupling step, using an excess of an activated carboxylic component to acylate an amino component, a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide, one or more aqueous extractions and optionally, a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step, referred to as step, in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions.

本发明涉及快速溶液合成肽的方法,该方法包括步骤-的重复循环:偶联步骤,使用过量的活化羧基成分酰化氨基成分,淬灭步骤,在该步骤中使用清除剂除去残余的活化羧基官能团,其中该清除剂还可以用于使生长中的肽去保护,一或多个水抽提和任选地,独立的去保护步骤,之后进行一或多次水抽提,其特征在于该方法包括至少一个步骤,称作步骤,在该步骤中包含自由阴离子或潜阴离子的胺或硫醇用作残余活化羧基官能团的清除剂。

The vicious cycle repeats itself ad infinitum. There is utmost urgency to stop causing this horror to yourself.

所以这种恶的循环自动地一再重复无休无止,所以妳该尽速的停止这些可怕的事情发生在妳自己身上。

Third, as to his relationships with the rest of the planet, the sceptics may repeat ad nauseam that an Obama presidency will not change America's status as a hyperpower and the disapproval that power evokes.

第三,至于他与地球上其它地区的关系,怀疑论者或许不断令人生厌地重复,奥巴马当选总统将不会改变美国作为超级强国的地位,以及由实力引起的反对。

The invention can repeatedly test the CT vessel contrast rebuild algorism quantitatively, to find out best parameter.

本发明便于操作和重复定量执行地检测CT血管造影术重建算法,以判断不同重建算法的优劣或同一种算法在不同的参数条件下的性能差异,进而找出最佳参数设置。

The teacher said angrily , I don't want you to repeat such as you told your classmates.

"老师愤怒地说:"我不要你重复你给同学讲的话。

It is the first time that no additional anode was adopted in the magnetic macroparticles filter. At the same time the auto-re-igniting system was designed for vacuum cathodic arc deposition instrument. It ignites the arc with an electronic component with high voltage and can auto distinguish weather the arc dies out or is on during the deposition via a main power supply feedback unit. The energy for igniting the arc is very less and thus can successfully prevent the film being contaminated.

设计制造的电子式真空阴极弧离子镀引弧器成功地实现了高压脉冲引弧,可以多次重复使用,所用能量较小,可以有效防止对所镀膜的污染,引弧可靠,能够自动识别电弧是否引燃,能够在熄弧后自动重新引弧,重新引弧的速度快,有效防止了熄弧引起的气压波动,使镀膜过程中气压非常稳定。

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