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FT-IR and curve-fitted results of amide I band are in good agreement with the analyses of CD spectra.

红外光谱定量结果表明大黄酸与人血清白蛋白结合改变了蛋白质的构象。

Using ultrasonic to measure the thickness of the cholecyst cliff in these patients. At the same time, we measure their breadth of the portal vein and the level of the ascites, and examine the level of ALB、TBil、AST、ALT in blood serum.

采用超声观察178例肝硬化患者的胆囊壁厚度和腹水检查、门静脉宽度、血清白蛋白水平、总胆红素水平以及门冬氨酸氨基转移酶、丙氨酸氨基转移酶水平。

Affer 4 to 8 weeks, the serum n itric oxide, plasma endotheliolysin, cGMP and lipid peroxide were meas ured and morphologic change of renal tissue examined.

为探讨穿心莲API0134防治家兔系膜增殖性肾炎作用机理,采用高胆固醇饲料另加牛血清白蛋白一次性注射方法建立家兔M sPGN模型,观察4~8周,检测血清一氧化氮,血浆内皮素,环鸟苷酸(cGMP 脂质过氧化物以及肾组织形态学改变。

In this paper hydrophilic-lipophilic balance of salad oil-water system was determined by standard emulsifier, while the HLB value of monoglyceride was measured by oil-water system.

目前在生物医学领域已进行了白蛋白及胰岛素的控制释放的壳聚糖/海藻酸钠微胶囊研究,但其制备方法仅适合于实验室生产。

A series of study methods which can not research in this field begin to exert important affection. On the basis of the previous research, the following major innovative work was carried out in this dissertation.(1) Several methods including fluorescence spectra, CD and FT-IR specta methods were used to study the interaction of isoquinoline alkaloid、isoflavones and Rhein with serum albumin, and the results from every method was in good agreement;(2) The binding constants、the numbers of binding site、the binding region and the interaction force involving in the interaction of drugs and serum albumin were confirmed by Scatchard equation and Vant Hoff equation.

基于研究生物大分子与小分子相互作用具有极其重要的意义,本博士论文在前人工作的基础上,主要进行了以下几方面的创新性研究:(1)利用荧光光谱法、圆二色谱法、红外光谱法以及分子模拟等多种方法相结合研究了异喹啉类生物碱,异黄酮类化合物以及大黄酸与人血清白蛋白的相互作用,获得了准确一致的信息;(2)根据Scatchard方程和Vant Hoff方程详细研究计算了异喹啉类生物碱以及大黄酸与蛋白质结合机理包括结合常数、结合位点数、结合区域、结合力。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法;钙测定用间接法也较好,其次是偶氮砷Ⅲ法;磷测定使用磷钼酸紫外法结果较好;血糖测定用己糖激酶法结果较好;尿素测定用脲酶紫外速率法结果较好;肌酐用酶法结果较好;白蛋白测定用溴甲酚绿法较好;胆红素测定用酶法结果好,其次是钒酸氧化法;碱性磷酸酶测定使用AMP缓冲液结果较好;淀粉酶测定酶法底物法和碘比色法变异系数均较大,前者稍小,合格率后者稍高,各实验室可根据自己的条件加以选用。

Serum and urinary creatinine, urine albumin excretion and NAG were determined photometrically. Urine γ-GT was assayed spectrophotometrically, and urinary B2M was obtained by radioimmunoassay.

每位研究对象均收集24小时尿液样本,测定血清及尿液中肌酐酸浓度、尿中白蛋白排除量、以及尿中 NAG、B2M、γ-GT的活性(以对肌酐酸浓度的比值表示)。

Liver function tests (biochemical marker: GOT, GPT, SALP, total protein, total albumin, TG, Cholesterol; antioxidative enzymes: SOD, catalase GSH, GSH-Px, GSH-Rd, MDA-TBA) were performed, and liver and body weights were measured.

肝功能的评估分别有ALT、AST、碱性磷酸、总蛋白、白蛋白、三酸甘油脂、胆固醇,以及抗氧化评估:超氧歧化、过氧化氢、GSH-Px、GSH-Rd、谷胱甘、MDA-TBA,并且测量小鼠体重及肝重。

The protective effect against hepatic damage was evaluated by measuring alanine aminotransferase, aspartate transaminase, total bilirubin, total bile acid, albumin, prothrombin time and child-pugh score at admission and after treatment.

同时监测治疗前后肝功能指标血丙氨酸转氨酶、天冬氨酸转氨酶、总胆红素、总胆汁酸、白蛋白、凝血酶原时间及child-push评分并进行对照比较。

Two haptens of mono-p-nitrophenyl phosphonates were designed and synthesized according to the structural features of the substrate and its hydrolytic transition state. The phosphonate haptens were coupled to the carrier proteins bovine serum albumin and keyhole limpet hemocyanin .

以对硝基乙酸苯酯水解反应中间体的结构信息进行分子设计,经过Arbzov等五步化学反应合成了二种对硝基苯基类膦酸酯半抗原,将其与载体蛋白(牛血清白蛋白BSA和钥孔〓血兰素KLH)偶联,制得了具不同抗原价(10~60)的半人工合成全抗原。

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