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This experiment take one-day-old chickens as the object of study, we apply cell raise technologyand MTT method, the indirect enzyme-lined immunosorbent assay, the immunity enzymehistochemistry methods andα-naphthyl acetate esterase etc..

本实验以1日龄雏鸡为研究对象,应用细胞培养技术和MTT法、间接酶联免疫吸附实验、免疫酶组织化学和组织酸性α-醋酸萘酯酶染色法等方法。

A simple one-step procedure was described for the synthesis of mesoporous monolith and composite sphere, in which hydrolyzation of tetraethyl orthosilicate and polymerization of urea with formaldehyde occurred at the same time.

利用酸性条件下正硅酸乙酯的水解和脲醛树脂的聚合反应同时一步原位进行的方法合成了二氧化硅复合粉体(包括核壳微球结构和网状结构)和块体凝胶材料。

Objective To overexpress human acidic fibroblast growth factor in Escherichia coli and study the biological activities of recombinant aFGF.

目的:探讨人酸性成纤维细胞生长因子(acidic fibroblast growth factor, aFGF)在大肠杆菌中高效表达的方法及重组人aFGF的生物学活性。

Fluorophore-assisted carbohydrate electrophoresis was used to analyse pectic oligosaccharides. The influence of different factors on the separation of oligosaccharides by FACE was investigated.

荧光辅助糖电泳是首先用荧光衍生化试剂对糖类分子的还原端进行衍生化标记,然后在一定浓度的聚丙烯酰胺凝胶上进行分离的分析方法,可同时分析中性糖与酸性糖。

A persulphate oxidation method in acid medium for decomposition and analysis of dissolved total phosphorus in seawater was proposed.

依此,建立酸性过硫酸盐氧化法分解测定海水中溶解态总磷的方法。

Melamine pyrophosphate can synthesized by three means:(1) prepared by pyrophosphoric acid and melamine in low temperature.(2) prepared by pyrophosphate and melamine in acid.(3) prepared in solid phase.

焦磷酸三聚氰胺的合成一般由三种方法:(1)由焦磷酸在低温下直接与三聚氰胺作用合成法;(2)酸性溶液中由焦磷酸盐合成法;(3)固相合成法。

DHCC Constance in the chemistry belongs to the onlighted Cyclopentanoperhydrophenanthrene when it reacted together with the acid Constance it could transform into the fluorescence phenol.we did the flouric determination for the DHCC, in which treated by the ethanol-sulfuric acid or acetic anhydride-methylene chloride-sulfuric acid, acquired standard curve in the 0-40μg/ml presented the liner relet...

DHCC类物质在化学上属于不发光的环戎烷多氢菲类,但与酸性物质一起反应时,可转化为产生荧光的酚类。用乙醇硫—酸或乙酸酐—二氯甲烷—硫酸处理后的DHCC进行荧光检测,获得标准曲线0—40μg/ml范围内呈线性关系,检出的最高灵敏度可达300ng/ml。该方法可用于分离后的DHCC及单一种DHCC代谢物的定性及定量分析。

In order to minimize the pollution of the environment with waste acids and/or waste acidic salt solution, following measures were taken:(1) Instead of using excess sulfuric acid, sulfonation reaction was carried out in the presence of excess toluene and the water produced during the sulphonation was removed by azeotropic distillation.

在CLT酸生产中,为了使废酸和酸性废盐水对环境的污染减少到最低程度,采取了如下的措施:(1)与使用过量硫酸相反,磺化反应是在过量甲苯的存在下进行,同时用共沸蒸馏方法移出了磺化过程中产生的水。

In the experimental study section, the rats were divided into four groups, including the normal control, the model, the Piracetam, the QPC, and copied to AD model. After the treatment, we observed the effects on the AD rats' ability of leaning and memorizing, detecting the TChE activity in whole blood and the expression levels of the related biochemical indicators of As includeing GFAP、NGF and BDNF of the AD rats' brain hippocampus, then compared the QPC with other groups to reveal the mechanism of action of the QPC treating AD to bright.

在动物实验部分,我们通过塑造AD动物模型,设立正常对照组、模型组、脑复康组和强脾益智胶囊组,观察强脾益智胶囊对AD模型大鼠空间学习记忆能力的影响,检测AD大鼠全血、海马匀浆液中乙酰胆碱酯酶活性和通过免疫组化的方法检测胶质纤维酸性蛋白、神经生长因子、和脑源性神经营养因子的表达情况来反映星形胶质细胞的增值情况,以期揭示强脾益智胶囊对AD的治疗作用机理。

MethodsThe alkaloid was extracted by chlorolform from Pinellia ternate and tested at 417nm with the spectroscopy by acid dye.

方法采用氯仿提取,依据酸性染料比色法的原理,在417 nm波长下测定,并作方差分析。

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