连接的

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

To be tricked into believing something that is not true 上……的当;受……的骗 Don't fall for the fair words of the door-to-door businessmen. That was the summer I worked at the fairground and met and fell for Lucy.21. Serve sb. right: to be a good punishment for sb.活该 You have missed so good a chance. It served you right to suffer the consequence now.

要点：该句为并列复合句，由 whereupon 连接两个并列句，而后一个分句中，又由 even though 引导了一个让步状语从句，意为"即使，尽管"， fair price 指的是公平价格，这不是一个具体的价格，而是指卖方原则上应得到的价格，即卖方能得到的适当的利润或对投入的资本能有适当收益的价格。

The Internet switching service method for providing an Internet switching service to a subscriber connected to the Internet (214) to make an Internet telephone call by using an ID of a receiver are achieved by inputting the ID of a receiver who the subscriber wishes to communicate with, searching a data base (202) where a plurality of IDs and communications address data of the IDs are previously stored, for communications address data corresponding to the IDs, sending the searched communications address data to the subscriber, and the subscriber performing a telephone call, a fascimile transmission or sending a post by using the searched communications address data.

对与因特网(214)连接的用户提供因特网交换业务以利用接收方ID进行因特网电话呼叫的因特网交换业务方法，通过下列步骤实现：输入该用户想与之通信的接收方ID，对先前存储有多个ID和这些ID的通信地址数据的数据库(202)搜索与该ID对应的通信地址数据，将搜索到的通信地址数据送给用户，以及该用户通过用搜索到的通信地址数据进行电话呼叫、传真发送或发送邮件。

Along with of microprocessor of development, the urgent demand have to match with it mutually of connect the issue that ocular exterior measure the equipments directly, root for a living through applied personnel in technique in science, find out a kind of exits already of but the past was output the equipments by the tiny electric current, electric voltage that people neglect, namely the high man in Rogowski electric current spreads to feel machine with divide to press the machine, its output's is several 100 peaceful electric current s/ V electric voltage can control with tiny model protection directly the equipments pick up a people, satisfying the request that protection control measure, and basic dissolves traditional electromagnetism type the electric current, electric voltage feels with each other saturated weakness in machine.

随着微机的发展，迫切要求有配合它的相互连接的问题，眼球的外部测量设备直接，根为口奔驰，通过应用型人才，在技术科学，找到了一种已经退出的，但过去仅仅是输出设备是由微小的电流，电压，人们忽视，即高文在罗柯夫斯基电流差突然觉得机器与隔膜，迫使机，其输出的是几个100和平电流s音频/视频电电压可控制与微小模型直接保护的设备，接了一个人，满足了要求保护控制措施，并基本溶化传统的电磁式电流，电压觉得与对方饱和疲软机器。

In order to guarantee canned and the seal movement with has the idle time movement the turnplate operating time operating time to be suitable, while has designed especially six V-belt pulleys which connects with the turnplate, has designed six V-belt pulleys, in order to driving plate after is separated from under the turnplate six to discuss, enters another six theory, drives on the turnplate the axis rotation, completes carries on canned and the seal to the idle time on the turnplate beverage bottle, after, when the turnplate revolves once more, canned and the seal work stops, will enter the idle time condition again until the next time turnplate.

为保证罐装和封口的运动与具有停歇运动的转台工作时间适当，特在设计了一个与转台连接的六槽轮的同时，多设计了一个六槽轮，以便主动拨盘在脱离转台下的六槽论后，进入另一个六槽论，带动转台上的轴转动，完成对停歇在转台上的酒瓶进行罐装和封口，完后，当转台再次运转时，罐装和封口工作停止，直到下次转台再进入停歇状态。

The present invention relates to compounds of formula wherein R is hydrogen, lower alkyl, lower alkoxy, halogen or lower alkyl substituted by halogen; R is hydrogen or halogen; or R and R are together with the carbon atom to which they are attached -CH=CH-CH=CH-; R is hydrogen, lower alkyl, phenyl optionally substituted by halogen, or is hetaryl, optionally substituted by lower alkyl; R is hydrogen, lower alkyl, phenyl, benzyl, lower alkenyl, lower alkoxy, phenyloxy, benzyloxy, S-lower alkyl, halogen, CN, lower alkyl substituted by halogen or O-lower alkyl substituted by halogen; R is hydrogen or lower alkyl, aryl is phenyl or naphthyl; n is 1, 2 or 3; m is 1 or 2; and pharmaceutically acceptable acid addition salts and tautomeric forms thereof.

本发明涉及式化合物及其可药用的酸加成盐和互变异构形式，其中R 1 是氢、低级烷基、低级烷氧基、卤素或被卤素取代的低级烷基；R是氢或卤素；或R 1 和R与它们连接的碳原子一起是-CH＝CH-CH＝CH-；R 2 是氢、低级烷基、任选被卤素取代的苯基或者是任选被低级烷基取代的杂芳基；R 3 是氢、低级烷基、苯基、苄基、低级链烯基、低级烷氧基、苯氧基、苄氧基、S-低级烷基、卤素、CN、被卤素取代的低级烷基或被卤素取代的O-低级烷基；R 4 是氢或低级烷基，芳基是苯基或萘基；n是1、2或3；m是1或2。

The deformation of conductive particles in pure metal connected by anisotropically conductive adhesives has been investigated using finite element analysis. The results indicate that the increment in stresses of the conductive particles begins at the contact area toward the boundary through the centers of conductive particles when the reduction in height increases. When stress is over the yield strength, the similar behavior was observed. So the deformation of conductive particles is not uniform, which means that the deformation of conductive particles is the same as the stress does.

采用有限元模拟分析了各向异性导电胶连接的纯金属导电颗粒的变形过程，研究结果表明，随着压下量的增加，导电颗粒的应力增加由接触面开始，经中心区域向导电颗粒的周边扩展，大于屈服强度的应力变化与此相似，因此导电颗粒的变形是不均匀的，即导电颗粒的变形由接触面开始，经中心区域向周边区域进行。

Methods RGD peptide were through direct covalent linkage attached on micro-channel surface with 1-ethyl-3- 3-dimethylaminopropyl carbodiimide hydrochloride and N-hydroxysuccinimide to A549 cells.

方法采用化学共价连接的方法固定RGD多肽，建立由流体控制系统、免疫反应系统构成的细胞识别芯片系统，用于细胞的筛选/捕获；选用不同密度的细胞进行进样试验，考察不同细胞密度对通道内细胞数量的影响，并讨论不同的孵育时间对细胞捕获的影响。

Methods RGD peptide were through direct covalent linkage attached on micro-channel surface with 1-ethyl-3- 1 3-dimethylaminopropyl carbodiimide hydrochloride and N- hydroxysuccinimide to A549 cells.

采用化学共价连接的方法固定RGD多肽，建立南流体控制系统、免疫反应系统构成的细胞识别芯片系统，用于细胞的筛选／捕获；选用不同密度的细胞进行进样试验，考察不同细胞密度对通道内细胞数量的影响，并讨论不同的孵育时间对细胞捕获的影响。

The results indicate that the foamed metals have the structure of dodecahedron with pentagonum casement toward space outspread. The framework self is the tube shape channels, which are cavate and nonentirely sealing. The whole framework is a maze structure connecting by this kind of channels. Folds and hollows besprinkle the surface of foamed nickel skeleton.

结果表明用本文方法制备的发泡金属是一种向空间延伸的具有五边形窗的十二面体结构，发泡金属骨架本身是中空的、非完全封闭式的管状&通道&，整个骨架就是由这种&通道&连接的&迷宫&式的结构。

He knows that he's one of the mostdominant players in the NBA right now.

我知道他现在是联盟里最有统治力的球员之一了。

Using analysis method of BCG , the dissertation analyzed the management of voluntary service organizations .

利用BCG分析方法对我国的志愿服务组织管理进行了分析。