连接两物的

Methods: Two sets of primers were designed according to the cyclostyle of pcDNA3.1-HBV-YVDD, and the full-length of HBV genome with different enzyme digestin spot was amplified.

以pcDNA3.1-HBV-YVDD为模板设计两对引物，扩增带不同酶切位点的HBV全基因，扩增产物纯化后，经酶切、连接，得到pcDNA3.1-HBV-YVDD重组体。

A washer has a body having an axis and provided with a first bearing face surface located at one axial side and adapted to cooperate with a nut, a second bearing face surface located at an opposite axial side and adapted to cooperate with an object, at least one third turning resistant surface adapted to cooperate with a thread of the bolt, the body having at least one resistive point arranged so that when the nut is turned and turns the bolt the body of the washer stops the bolt from turning and thereby the nut creates a pull on the bolt which elongates the bolt in an axial direction and applies to the body of the washer an axial force which overcomes the at least one resistive point so that a portion of the body of the washer is allowed to be pulled axially when the bolt elongates.

一种放在螺帽与物体之间的垫圈，螺帽与螺栓螺纹连接，螺栓插入在包括两个装配的部件的物体内，垫圈具有主体，和设有第一支承面表面，位于一个轴向侧面和与螺帽配合，第二支承面表面，位于相对的轴向侧面和与物体配合，以及至少一个第三转动阻力表面，与螺栓的螺纹配合，从而使螺帽以给定的扭矩在一个方向转动同时垫圈的主体沿相对方向接收给定的力时，仅有螺帽转动，以紧固或松开螺栓，而垫圈的主体和螺栓保持静止。

In her eyes, because that is the doorway toher heart * the place where love resides.

女人的漂亮必須從她的眼睛中去看，因為那是她心靈的窗戶和愛居住的地方

I will send some postcards to you.

我会向你发送一些明信片。