进化

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Sequence analysis showed that the size of 16S rRNA gene was in 1235 bp, and lacking several regions which regarded as eukaryotic sequence, was more similar with prokaryotes than eukaryotes in sequence structural feature; it could be implied that the microsporidia had split from ancient eukaryotes very early in time on the phylogenetic tree.

序列分析表明，16SrRNA基因大小为1235bp，缺乏多个被认为是真核生物序列的区域，序列结构特征更多地类似于原核生物的，在进化上，推测微孢子虫在很早以前与原始真核生物产生分歧；分子进化分析发现，同属的微孢子虫可以处于系统进化树不同的分枝。

I suspect that women evolved breasts which didn't disappear after breast feeding, and that this led to the evolution of men's lust for breasts, and that following this, nulliparous breasts evolved in response to the new lust.

我猜测先是女人进化出不会在母乳喂养结束后消失的乳房，然后这导致了男人进化产生对于乳房的色欲，接着，未产妇对这种新出现的色欲做出回应，进化出了乳房。

The phyletic evolution analyzing of this PI sequence and PI sequence from other plants by DNAman software, we found that buckwheat and Amaranthus hypochondriacus merge firstly, then merge with Momordica, Sambucus, Solanum, etc. Meeting with the phyletic evolution relation assess by morphology.

应用DNAman软件对荞麦蛋白酶抑制剂的氨基酸序列及从GenBank中获取的其他植物蛋白酶抑制剂序列进行系统进化分析，发现荞麦与苋属植物最先聚类合并，在进化上显示靠得最近，接着与百日草类群聚合，然后再与苦瓜属、接骨木属、柑橘属、茄属植物聚合，符合按照形态学进行判定的系统进化关系。

The result of clustering based on quantitative data of vessel elements suggests that the Sonneratia species evolved into two clades, one including S. caseoalris and S. paracaseolaris that has the wider vessel and lower pore density suggesting a high conducting efficiency, the other including S. alba, S. ovata, S. apetala and S. hainanensis that has the narrower vessel and higher pore density resulting in high conducting safety.

导管数量特徵的聚类分析可以推测海桑属植物沿两支进化，一支进化为水分输导效率高的种类（即导管直径宽和输导面积大，管孔密度小，如海桑和拟海桑），另一支进化为水分输导安全性高的种类（即导管直径窄、输导面积小，管孔密度大，如杯萼海桑、卵叶海桑、无瓣海桑、海南海桑）。

The result of clustering based on quantitative data of vessel elements suggests that the Sonneratia species evolved into two clades,one including S. aseoalris and S.paracaseolaris that has the wider vessel and lower pore density sug esting a high conducting eficiency,the other including S.alba,S. ovata,S.apetala and S.hainanensis that has the narrower vessel and higher pore density resulting in high conducting safety.

导管数量特征的聚类分析可以推测海桑属植物沿两支进化，一支进化为水分输导效率高的种类(即导管直径宽和输导面积大，管孔密度小，如海桑和拟海桑)，另一支进化为水分输导安全性高的种类(即导管直径窄、输导面积小，管孔密度大，如杯萼海桑、卵叶海桑、无瓣海桑、海南海桑)。

Different from the sole-population evolution, the multi-population evolution partitioned the initial population into umpty sub-populations. Each sub-population evolved independently according to certain schema. In proper situation, the sub-populations would exchange information among themselves, to maintain the population diversity.

与单一种群进化不同的是，多种群进化将初始种群划分为若干子种群，各个子种群按照一定的模式独立进化，适当的时候在子种群之间交换信息，从而维持种群的多样性。

The theory minutely analyze trait of evolutionary programming arithmetic and state variables breakout problem in solving reactive power optimization problem, link reactive power balance theory.. reactive power bed and subarea control theory and adjusted-voltage material law of actual system. The theory first put forward a effective variability quantity modified measure, escape unfeasible problem in evolutionary programming, also make evolutionary programming arithmetic in solving reactive power optimization problem more quick and effective.

详细分析了进化规划算法的特点和在求解无功优化问题时存在的状态变量越限问题，结合无功平衡原理、无功分层分区控制原理以及实际系统中调压的具体规律，首次提出了一套有效的变异量修正措施，避免了进化规划的不可行问题，也使进化规划算法在求解无功优化问题时更加快速有效。

They found that most of the columbines' nectar spur length evolution happened during shifts in pollinators from bumblebees to hummingbirds, and from hummingbirds to hawkmoths. In between these shifts, evolution of the columbines' nectar spurs came to a halt.

Whittall和Hodges发现，在许多植物中，这种协同进化更具有&一边倒&的倾向，蜜管会随着采蜜者喙的长度而进化，随后这一过程会中止，只有新的采蜜者出现才能让进化过程重新启动。

It is also suggested that the evolution of seed dis-persal systems is mainly determined by a few key dispersers and plants, which may control the evolutionof related traits of other species.

动植物之间还可能发生在种、属或科水平以上的多物种的多配协同进化，或者通过关键种的协同进化来带动其他食果动物和植物相关性状的进化。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。