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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中,苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变,并筛选在自生生长时期、共生生长时期的表型,以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因,我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法,发现lsrA基因主要在根瘤侵染区开始表达,表达时序也在侵染阶段左右,但晚于bacA基因表达;LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体,nifA和fixK基因的转录水平降低,nifH基因的转录被完全阻断,因此LsrA蛋白为根瘤发育所必需,是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因,并研究了它的调节特性。oxyR突变后,苜蓿中华根瘤菌对过氧化氢敏感性提高,适应性降低。

The 20S catalytic core of the 26S proteasome has the shape of a barrel made of four rings which are composed of seven differentαsubunitsα_1-α_7 at two outer rings and seven differentβsubunitsβ_1-β_7 at two inner rings at which the catalytic sites locate. Three of theβsubunits contain proteolyses sites, which are sequestered in the hollow interior of the 20S particle. Substrates enter the 20S through a narrow channel formed by theβsubunits, whose N-termini, depending on their conformation, can either obstruct or allow substrate entry and thus function as a gate. This entry channel is narrow and only permits passage of unfolded, linearized polypeptides.

哺乳动物26S蛋白酶体是由一个20S催化颗粒(catalytic particle,CP)和两个19S调节颗粒(regulatory particle,RP)组成的ATP(adenosine-triphosphate,ATP)依赖性蛋白水解酶复合体。20S CP是26S蛋白酶体的催化核心,它是由4个圆环堆叠形成的桶状复合物,其中两侧外环每个环是由α_1-α_7亚基组成,两个内环每个环是由β_1-β_7亚基组成,4个环的中央形成一个狭窄的内腔。2个β内环形成了20S CP的催化中心空腔,其内壁为催化活性位点所在地;外侧α环中心的孔道是底物到达催化中心空腔的通道,一般被α亚基上的N末端所封闭,阻止胞内非目的靶蛋白进入20S CP内被降解破坏。

The lighting is fitted with movement sensors so that it switches off if no-one is using that area and the new office furniture has been produced in the most environmentally friendly way possible New heating and air conditioning plant serving the meeting rooms area has been installed to reduce energy usage and improve efficiency and, over the next twelve months, new air conditioning units will be installed in the remainder of the building.

的照明装有移动传感器,以便它会自动关闭,如果没有人使用这一领域和新的办公家具已在环境最友好的方式可能的新的供暖和空调厂会议室,服务区已被安装到生产减少能源使用,提高效率,并在未来12个月,新的空气调节装置将被安装在建筑物的其余部分。

Fuzzy logic control, as a newly automatic control strategy, has been applied in industrial occasion, has insensitiveness and fast response to controlled process.

由于静压储能传动系统工作时系统结构和系数都随着负载工况的变化而变化,采用常规的基于被控对象精确数学模型的控制策略不易或不能有效地完成对被控对象的调节。

Most point-and-shoot cameras have a simple exposure override facility, normally allowing you to overexpose or underexpose your picture. So if the subject is predominantly dark, experiment by overexposing to compensate. If the subject is predominantly light, then underexposure is the way to go. Try taking a test picture, look at it on the screen on the back of your camera, check the histogram, and adjust your exposure compensation. Don't be afraid to shoot four or five versions, as the LCD screen is not always accurate. You can delete the bad pictures later.

大多自动数码相机都具有曝光优先功能,一般都允许你在拍照时过度曝光或曝光不足,如果被拍摄物体整体光线比较强,可以减弱曝光强度,如果被拍摄物体整体光线比较弱,可以选择过度曝光,试着多拍摄几张照片,通过相机背部的液晶屏观察柱状图,调节曝光补偿等级,因为液晶屏的显示效果不很精确,别嫌麻烦,多拍几张照片,之后,对于效果不理想的照片可以删除。

In this paper the dynamic characteristic of quartz lamp heater and silicon controlled rectifier installations was addressed through theoretic analysis and test, and the static characteristic of quartz lamp heater was investigated through system identification; In foundation of studying controlled object's characteristic a kind of design procedure on nonlinear PID controller was found, it is applicable to arbitrary quartz lamp heating system.

本文通过理论分析和试验测试研究了可控硅功率调节装置和石英灯加热器的动特性,提出了通过系统识别的方法研究石英灯加热器的静特性;在研究被控对象特性基础上找到了对任意一个石英灯加热器组成的被控对象进行非线性PID控制器的设计方法。

TWT's two thousand various products are widely used in hydraulic generations,city plumbing ,wastewater treatment facilities,petroleum and chemical industries ,heating supplies ,and any other water related business.Among them,Water butterfly valves,DB flange butterfly valves and Tri-eccentric compound ring metal seated butterfly valves won the State New Products awards in 1994,2000,and 2001,respectively. The butterfly valve series carryings the "TWT" brand name was selected for the Famous Brand Product of Tianjin Award by the Tianjin government .

上述产品广泛应用于水源工程、城市给排水、污水处理、发电、石油、化工、供热、船舶、医药、冶金等行业管路系统中作为截流和调节设备使用,其中对夹式蝶阀、DB型法兰蝶阀、三偏心复合圈金属密封蝶阀、软密封明杆闸阀分别被评为1994年度、2000、2001、2002年度国家级新产品,"TWT"牌系列蝶阀被天津市政府授予"天津市名牌产品"、"外地市场受欢迎的天津产品"称号。

HUP+LBNP was an effective approach to examine the regulatory function of cardiovascular system,and the sphygmogram could not be used in studying the regulatory function of cardiovascular system during HUP+LBNP.

HUT+LBNP是一种负荷量较高的立位耐力检查方法,可充分暴露被检者在立位中的心血管调节功能;脉图检测方法不能作为评价被试者HUT+LBNP耐力的方法

In the adult, cholinergic neurons in the laterodorsal and pedunculopontine tegmental have been implicated in the generation and maintenance of REMS.

虽然在成年人和动物,胆碱能神经元被认为是主要的REM睡眠的调节神经元,已有实验证实脑桥网状结构及脚桥被盖核胆碱能神经元在REMS的触发和维持中起着关键作用,然而,胆碱能神经元对于幼年期REM睡眠的作用仍有待进一步的研究。

The purpose of fogging is to relax accommodation by creating an artificial myopia in all eyes regardless of their original refractive status.

9雾视法的目的在于使被测眼的调节松弛,不论其原来的屈光状态如何,被测眼均处于人为的近视状态。

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He is the most remarkable man that I have ever known.

他是我知道的人中最值得注意的人。

Increased appearance of lymphocele was noticed in patients with diabetic nephropathy, congenital malformations of the urinary tract, and inflammatory diseases, including glomerulopathy and extraglomerular ones, after high-voltage radiotherapy and after removal of the renal graft.

经过高压放射疗法和切除移植肾后,在糖尿病肾病患者、泌尿系统先天性畸形患者和炎症患者身上发现淋巴囊肿有所增加,包括肾小球病和球外疾病等。

Pagans and partygoers greeted the summer solstice at the ancient stone circle of Stonehenge.

异教徒和社交聚会常客在史前巨石柱的古老的石圈附近庆祝夏至。