袋熊

While Wombat rested Mother Kangaroo kept looking around.

当老袋熊在休息时，袋鼠妈妈四处张望着。

The Wombat deposits square poos on logs, rocks and even upright sticks that it uses tomark its territory.

袋熊将粪便堆积在木头，石块，甚至树枝上表明自己的领地。

"Ouch! Watch were you're going" yelled Wombat.

"哎哟！看着点！"老袋熊大声嚷道。

This might look like a scene from Mad Max but, in reality, it is the front line in the battle to protect the health and the future of the southern hairy-nosed wombat.

看起来好像飚车，不过，这却是保护袋熊战斗的最前线。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

From Fraser Island and its tenacious packs of dingoes to the Australian bush, alive with koalas and wombats.

弗雷泽岛上的灌木丛林里生活着成群顽强的澳洲野犬，其中也居住着树袋熊和袋熊。

There's just so much for children to see and do: like swimming with sea lions and dolphins at Baird Bay; sliding down sand dunes on Kangaroo Island; noodling for opals in Coober Pedy and patting koalas, kangaroos and more in wildlife parks just outside Adelaide.

那里可以让孩子看的做的实在是太多了：在Baird Bay和海狮，海豚一起游泳；从袋鼠岛的沙丘上滑下；在Coober Pedy 里面对猫眼石毫不知情以及拍拍数袋熊，袋鼠和其他野生动物园里动物的小脑袋。

There's just so much for children to see and do: like swimming with sea lio and dolphi at Baird Bay; sliding down sand dunes on Kangaroo Island; noodling for opals in Coober Pedy and patting koalas, kangaroos and more in wildlife parks just outside Adelaide.

那里可以让孩子看的做的实在是太多了：在Baird Bay和海狮，海豚一起游泳；从袋鼠岛的沙丘上滑下；在Coober Pedy 里面对猫眼石毫不知情以及拍拍数袋熊，袋鼠和其他野生动物园里动物的小脑袋。

The Wombats:Yeah. We listen to all kinds of music.

袋熊：是的。我们听各种风格的音乐。

Wombats are very strong animals, and they scratch and bite animals that might try to eat them.

袋熊是非常强壮的动物，会抓伤、啃咬想猎食它们的动物。

In her eyes, because that is the doorway toher heart * the place where love resides.

女人的漂亮必須從她的眼睛中去看，因為那是她心靈的窗戶和愛居住的地方

I will send some postcards to you.

我会向你发送一些明信片。