表达

After analyzing the merit and lack of 9I model, the main idea is proposed: to distill some basic topological relations from 9I model, of which the composite topological relations of complex objects are made up. In detail, the composite topological relationships between region and region, line and line, line and region are particularly discussed;(2)Analyze the characteristics and inner relations of cadastral features. According to the expressional model of complex topological relations, the author discovers the possible topological relations between parcels, parcel lines and parcel points. In addition, sum up the general topological relationship rules of cadastral data.(3) Then the topological relations model is extended to the spatio-temporal data. Time in cadastral database and change semantic are discussed. It's redefined that spatio-temporal topological relations is composed of time, location, status and event relation. Spatio-temporal topological relationships can be represented by extended 4I model. Taking cadastral parcels as example, there are different possible spatio-temporal topological relationships between parcels when they are extended, shrunken, split, merged or others. Thus, some deductions are drawn that, for example, if parcels have be coexisting for some time they are impossible to be overlapped. Furthermore, the parents of parcels alternated and their possible change types can be detected by their spatio-temporal topological relationships.(4) Before carrying out the experiment, the calculation method must be designed.

具体研究工作及成果如下：（1）根据点集拓扑理论中与拓扑空间相关的概念，本文对空间的点、线、面进行了重新定义，以区分对象的复杂性；分析了9I模型在表示简单对象方面的优点，以及在表达复杂对象上的不足，阐述了本文的研究思路和解决方案，并以复杂的面面、线线和线面为例详细探讨了复合拓扑关系的表达方法；（2）分析了地籍权属数据和地类数据的内涵、特点和要素之间的联系，根据复杂对象的拓扑关系表达模型，本文讨论了宗地、界址线、界址点之间，图斑、线状地物、零星地类之间可能存在的拓扑关系和形式化表达方法，归纳总结了地籍对象的拓扑关系满足的约束性规则；（3）将拓扑关系的研究延伸到时空领域，从地籍对象的时态性出发，本文阐述了地籍数据中时间的含义和变化语义描述的需要；从时间、位置、状态、事件等之间的拓扑关系方面，重新系统性地进行了时空拓扑关系的定义；研究了一维时间和二维空间的集成表达方式，以宗地、界址线为例，分析了变更前后不同时间区间里可能的拓扑关系，探讨了时空拓扑关系对时空变化操作类型和变化语义的推理方法和意义；（4）为实现空间拓扑关系的查询分析等应用，本文研究了空间拓扑关系的计算方法。

A portion of ductal cells and individual serous acinic cells of the minor salivary gland also expressed Fas not only in membranous but also in cytoplasmic pattern.

结果发现，在正常鼻咽粘膜表面及隐窝的假复层纤毛柱状上皮细胞胞膜、部分小涎腺的导管上皮和个别浆液性腺泡上皮细胞的胞膜和胞浆Fas阳性表达；鳞状化生上皮的表层细胞胞膜有Fas表达，但基底细胞未见Fas表达；浸润的淋巴细胞也不表达Fas.45.9%（17/37）的鼻咽非角化性癌Fas阳性，但只有不足10%的癌细胞胞膜微弱表达Fas。

The percent of atresic follicular increase with dosage rising (P.05).The result of expression of Bax and Bcl-2 showed that the Bax expressed widely in every groups, and it enhanced with dosage, there is extreme significant difference between experiment group and control group (P.01),there is not difference between withdrawal 48 hours and 96 hours.

免疫组化法检测Bax和Bcl-2蛋白表达，结果Bax在各组普遍表达，并随镉剂量的加大而表达增强，实验组与对照组间差异极显著(P.01)，48h与96h两时间段间无差异；Bcl-2则普遍表达较弱或不表达，各组间无显著性差异。

Results showed:(1) The airway responsiveness of smoke control group was higher than that of normal control group, the airway responsiveness of smoke + exercise group was lower than that of smoke control group;(2) Plasma level of cortisol determined immediately after exercise was higher than that determined before exercise, no significant difference occurs between that determined next day morning after all exercise finished in contrast to that determined before exercise;(3) HE staining showed, there was severe chronic pulmonary inflammatory response in smoke control group, which was reduced in the smoke + exercise group;(4) chronic cigarette smoke downregulated the protein and mRNA expression of BKca in smooth muscles of bronchi and bronchioli, exercise increased the mRNA expression of BKca;(5) chronic cigarette smoke downregulated the protein and mRNA expression of Kv1. 5 in smooth muscles of bronchi and bronchioli, exercise increased both the protein and mRNA expression of Kv1. 5 in contrast to smoke control group in bronchioli and did not effect the expression of Kv1. 5 in bronchi;(6) chronic cigarette smoke induced upregulation of FIZZ1/RELMα in bronchial smooth muscle cells, exercise decreased these effect.

结果：(1)吸烟对照组的气道反应性明显增高，吸烟加运动应激可明显降低大鼠的气道反应性；(2)吸烟运动组运动后血浆皮质醇浓度明显高于运动前，但全部运动结束后次日晨测定的血浆皮质醇浓度与运动前无明显差异；(3)HE染色显示，吸烟对照组肺组织出现明显的慢性炎症反应，吸烟加运动应激组比吸烟对照组炎症反应轻；(4)慢性吸烟可降低大鼠大气道和小气道BKca mRNA和蛋白表达，而吸烟加运动应激可使BKca mRNA表达上调，但对小气道的蛋白表达无影响；(5)慢性吸烟可降低大鼠大气道和小气道Kv1.5蛋白和mRNA表达，在小气道吸烟加运动应激可减轻这种作用，大气道则无作用；(6)吸烟使支气管平滑肌的FIZZ1/RELMα蛋白表达明显增强，而吸烟加运动组的RELMα蛋白及mRNA均明显减弱。

Results］ the expression of cox-2 in hepatocellular carcinoma and its adjacent tissues with cirrhosis were 70.0% and 92.0% respectively, and they were higher than that in normal liver tissue (20.0%). the cox-2 expression in carcinoma adjacent tissues with cirrhosis was higher than that in hcc. expression of cox-2 in well differential hcc was higher than that in poor differential hcc, and higher in case with metastasis than that without metastasis(p＜0.01).the expression of cox-2 in hcc did not relate to tumor size, tumor capsula and the level of afp.

结果］ cox-2蛋白在hcc、癌旁肝硬化中表达阳性率分别为70.0%和92.0%，显著高于在正常肝组织中的20.0%（p＜0.05），而cox-2在癌旁肝硬化组织中的表达高于hcc组织。cox-2蛋白在分化好hcc中的表达显著高于分化差的hcc（p＜0.05），转移组中cox-2蛋白表达显著高于无转移组（p＜0.01）；而cox-2蛋白的表达与肿瘤大小、有无包膜及afp水平无关。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

Among the 3 novel sequences, C24 sequence and C57 sequence were expressed both in cataractous group and in normal group; C24 sequence was expressed higher in cataractous group than that in the normal group, but C57 sequence had no significant difference in normal and cataractous group; C40 sequence was expressed in the cataractous lens epithelium but the expression in normal lens epithelium was not seen.

三个新序列中，C24号和C57号序列在年龄相关性白内障和正常晶状体上皮细胞中均有表达，C24号序列的表达增高；C57号序列在正常组和白内障组中的表达无差异。C40号序列在年龄相关性白内障晶状体上皮细胞中有表达，但其在正常晶状体上皮细胞中未见表达。

Results: Through analysis gene expression profiling of cholangiocarcinoma metastasis tissues and normal tissues, 232 genes differentially expressed in 4 samples were identified, 8 genes expression up-regulated and 6 genes down-regulated coinstantaneous.

结果 胆管转移癌与正常胆管粘膜的基因表达谱分析，有232条基因表达有明显差异，与转移相关基因一致的表达14条，8条表达上调，6条表达下调。

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

An amount increase ofPAI-3 andK10 was observed after theKCswere cultured for48 h in high calcium medium,butat72 h the exprssion ofPAI-3 decreased. The positive stain ofPAI-3 wasmainly localized in perinuclearmembrane in lowcalcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope ofterminally differentiated keratinocytes.

结果 高Ca2+浓度下培养24 h后，分化标记物K10弱阳性表达， PAI-3呈阳性表达；培养48 h后， K10强阳性表达，而PAI-3的阳性表达明显增加， 72 h后，表达开始降低，在低Ca2+浓度下阳性染色主要位于细胞核膜周围而高Ca2+浓度下主要位于细胞质，同时， PAI-3存在终末分化KCs角质壳中。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。