融合虫

CWhen the fusion protein was co-culture with nematode,the analysis of fluorescence microscope showed the digestive system and the coelom of nematode could be detected green fluorescence, and the fluorescence intensity was time-and protein concentration-dependent.

其三，融合蛋白喂食线虫发现蛋白进入其整个消化道及其原体腔，且与喂食时间与蛋白浓度呈正相关关系。

The fused protein from pPIC9KBC/KM71 yeast strain had the insecticidal activity and could be digested by enterokinase in vifro.

酵母中表达的融合蛋白具有杀虫活性，并且可以被肠激酶进行体外切割。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

The effect of tat p53 on the growth of parasites was determined by counting the parasitemia changes between pre and post incubation of the fusion protein with the parasite culture for 12 h. results: tat p53 fusion protein could be transduced into p.f parasites, locating at trophozoit and late schizont stage, whereas p53 protein could not.

结果：tat p53融合蛋白可以突破红内期疟原虫各层细胞膜，进入虫体细胞内，并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体，而p53蛋白不能进入疟原虫内。

The effect of TATp53 on the growth of parasites was determined by counting the parasitemia changes between pre and postincubation of the fusion protein with the parasite culture for 12 h. RESULTS: TATp53 fusion protein could be transduced into P.f parasites, locating at trophozoit and lateschizont stage, whereas p53 protein could not.

结果：TATp53融合蛋白可以突破红内期疟原虫各层细胞膜，进入虫体细胞内，并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体，而p53蛋白不能进入疟原虫内。

Objective To obtain the functional fusion protein of rhoptry protein 2(ROP2) and major surface antigen 1 of Toxoplasma gondii in order to further prepare for the diagnosis and polyvalent vaccines of toxoplasmosis.

目的构建编码弓形虫RH株棒状体蛋白2(ROP2)和主要表面抗原1的重组表达质粒，纯化和复性的融合蛋白为弓形虫病快速诊断试剂盒及蛋白质疫苗的研制作准备。

In both male and female worms, the tegument showed focal or extensive swelling, fusion, vacuolization, erosion, peeling, and destruction of sensory structures.

雄虫的皮层结节肿大、破溃或从皮层上剥落；在雄虫和雌虫的体表可查见有局灶性或广泛的皮层肿胀、融合、空泡变化、糜烂和剥落，以及感觉结构的破坏。

The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外，还可见到多种形态异常的虫体，包括；虫体呈非典型二分裂；滋养体胀大，胞质中有团块状和不规则形状的嗜酸性物质；在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛；在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛；在一个母体细胞中含有3或4个子体细胞的雏形；胞质互相融合的4个滋养体的雏形；胞质互相触合的3个滋养体，胞质互相融合的4个滋养体；1对营二分裂的滋养体与另一个滋养休互相融合；2对营二分裂的滋养体互相融合；仅有1个细胞核的滋养体。

The CryCI gene and essential regulation elements cut from the plasmid pGF4ABC were inserted into yeast expression vector pPIC9K and plant expression vector pBI 121.1, so we got the secretive yeast expression plasmid pPIC9KBC and plant high-efficiency expression plasmid pGBIF4ABC, Fifty-two His+Muts transformants were obtained after the expression vector pPIC9KBC was introduced into Pichia pasoris, KM7I strain, by electroporation.

将pGF4ABC上的融合杀虫基因CryCI分别克隆到酵母表达载体pPIC9K和植物表达载体pBI121.1上，构建成融合杀虫基因的分泌型酵母表达载体pPIC9KBC和高效植物表达载体pGBIF4ABC。

The female prosopon body oval,body long 2.3-3.5mm,antenna 7,the body by the white cotton and kapok shape wax silk,many insect\'s wax silk often is fused densely in together,forms the cotton and kapok to be spherical,after the dewaxing the insect body becomes the purple red.

雌成虫体卵形，体长2.3—3.5mm，触角7节，体密被白色棉状蜡丝，多个虫的蜡丝常融合在一起，形成棉球状，去蜡后虫体成紫红色。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。