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Both the new peniculus 1 and the old haplokinety separated at the telophase.The two germinal rows germinal rowsfor both the daughter cells appeared almost at the same time;(2) The macronucleus became shorter and thicker, finally oblate during the division of the cell. Then the macronucleus restored its original shape, band-like form. The micronucleus were divided into two before the macronucleus division;(3) The original scopula and stalk contributed to the old cell. The new cell formed scopula gradually after the cell division.

同时,新仔虫的第一咽膜(P′1)也开始由老单毛基索复制,并在细胞分裂后期与老结构分离;(2)大核在虫体分裂过程中由长带状逐渐缩短变粗至扁圆形,于虫体即将分开时迅速拉长,然后分裂为二个新大核;小核分裂先于大核,在两仔虫口纤毛器即将分开时完成;(3)原帚胚及柄归属老仔虫,新仔虫的帚胚在虫体分裂后逐渐形成,柄内肌丝则在柄鞘形成后逐渐长出。

The additional supplements including glucose,ascorbic acid and insulin had the enhancement effects on C.andersoni development,while folic acid had no significantly effect on C. andersoni development and calcium pantothenate had inhibited effect.When added 50 mmol/L glucose,50μg/ml ascorbic acid,0.3 U/ml insulin into 10%FCS RPMI-1640 medium,the proliferation of C.andersoni had a great significantly increase,and the gene copies of C.andersoni were 8-fold compared with that in 10%FCS RPMI-1640 medium.

冻融法+蛋白酶K法+声裂法提纯隐孢子虫卵囊DNA效果最佳,其次为冻融法+声裂法和蛋白酶K法+声裂法,冻融法+蛋白酶K法效果最差。10%FCS是培养安氏隐孢子虫的最佳FCS浓度,葡萄糖、维生素C和胰岛素都具有促安氏隐孢子虫增殖作用,泛酸钙无促虫体增殖效果,而叶酸则抑制虫体增殖。10%FCS RPMI-1640培养液中加入50 mmol/L葡萄糖,50μg/ml维生素C和0.3 U/ml胰岛素促安氏隐孢子虫增殖效果最显著,虫体增殖8倍。

Trophozoite,macrogamout,microgamout, typeⅠmeront,typeⅡmeront and sporozoite in the development of C.andersoni were observed by TEM.The proliferation of C.andersoni was significantly inhibited by 1.25μg/ml NTZ and 0.31μg/ml GAs.Treated with 20μg/ml NTZ and 5μg/ml GAs respectively,the numbers of C.andersoni were only 0.29%and 0.66%compared with the untreated.

透射电镜在HCT-8细胞中观察到安氏隐孢子虫的滋养体、雌配子体、雄配子体、Ⅰ型裂殖体、Ⅱ型裂殖体和子孢子阶段。1.25μg/ml NTZ和0.31μg/ml GAs即可显著抑制HCT-8细胞中安氏隐孢子虫的增殖。20μg/ml NTZ和5μg/ml GAs作用后,虫体数量仅分别为未加药的0.29%和0.66%。

To observe the abnormal morphs of the trophozoite of Giardia lamblia in vitro axcenical cultivation. G. lamblia trophozoites were cultivated axenically in modified TYI-S-33 medium. Sediments, which contain the trophozoites, on the bottom of the culture tubes were collected at different time after inoculations.

目的 观察体外纯培养的蓝氏贾第鞭毛虫滋养体的异常形态,方法用改良TYI-S-33培养基培养贾第虫,在接种后不同时间取培养管底部虫体涂片,Giemsa's染色,在显微镜下观察虫体形态并照相。

The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外,还可见到多种形态异常的虫体,包括;虫体呈非典型二分裂;滋养体胀大,胞质中有团块状和不规则形状的嗜酸性物质;在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛;在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛;在一个母体细胞中含有3或4个子体细胞的雏形;胞质互相融合的4个滋养体的雏形;胞质互相触合的3个滋养体,胞质互相融合的4个滋养体;1对营二分裂的滋养体与另一个滋养休互相融合;2对营二分裂的滋养体互相融合;仅有1个细胞核的滋养体。

Experimental mice were exposed to Trypanosome evansi after injection by different doses of antigenic reservoir. The results showed that 20 μg dose produced 83% and 67% protection to 2 sample variations after 4 immunizations. However, 3 immunizing doses could not give good protection. No protective effects were detected when experimental animals immunized by single VSG exposed to isotype from different rabbits.

用不同剂量抗原库免疫小鼠后进行攻虫,结果显示:经四次免疫后,20μg 剂量对随机抽取的两个变异体100 条虫体的保护率分别达到 83%和 67%;改变免疫方法,经三次免疫后的保护效果不理想;用单一 VSG 抗原免疫小鼠,接种来自不同兔体的同型虫体,不能起到相应保护作用。

The research showed that:(1) Dendroctonus micans and Ips nitidus responded to pheromone of low concentration,which demonstrated that the olfactory response threshold of these two species to pheromone was very low,and the response value increased with the concentration.

研究结果表明:(1)云杉大小蠹与光臀八齿小蠹均能对其较低浓度的虫体信息素成分产生嗅觉反应,说明其对虫体信息素的感受阈值较低;且随着虫体信息素浓度的增大,反应值增加。

There is a polarized organelle, Golgi complex consisted of several saccules and some vesicles beside the nucleus and there are some osmiophilic bodies beneath the parasite pellicule.

结果表明,配子体是一渐进发育的单核、惰性虫体,其核旁有一个由扁平囊和泡状小体极性排列组成的细胞器———高尔基体,虫体被膜下有嗜锇小体。

Methods Eight cases of cerebral sparganosis treated at Huashan Hospital between 1982 and 1997 were analyzed retrospectively.

结果本病的病理特点为:1蚴虫虫体为实体,无体腔,具特征性体壁;2蚴虫虫体内散在分布的同心圆性圆形或椭圆形的石灰小体及单个肌纤维;3脑内有新旧不一的多发性小脓肿。

In certain range,the bioenrichment of the mealworm larvae to Zinc in reference to the dosage added in the fodder is accorded with cubic curve relation:Y=181.2517+20.9314X-0.4099X2+ 0.0025X3. And the enrichment ability of T. molitor larvae in reference to the weight is also accorded with the cubic model:Y=35.8325+61.4155X-10.2980X2+0.4434X3. The enrichment of the larvae to Zinc can get to a high level in 2 to 3 days. This is anadvantage to numerous rearing of the mealworm. But we do not find a good rearing way to enhance the rate of bioenrichment to Zinc. And the further research about this is anticipated.

第二部分:采用锌作为添加元素,考查黄粉虫对微量元素的富集规律,结果表明:(1)黄粉虫虫体锌含量在一定范围内随锌添加量增加而呈三次曲线关系,符合三次曲线方程:Y=181.2517+20.9314X-0.4099X~2+0.0025X~3;(2)黄粉虫幼虫对锌富集能力随虫体大小变化而变化,与虫大小亦呈三次曲线关系;Y=35.8325+61.4155X-10.2980X~2+0.4434X~3;(3)黄粉虫虫体锌含量随着锌饲料添加而迅速上升,可在短期2~3天内达到较高水平,对于以黄粉虫为材料进行富锌保健食品生产有很大优越性;(4)本试验并未找到一种较好的锌添加方法来提高锌富集量,这方面的研究有待继续。

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