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GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro, and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15'C for 8 h, normal infant mice could be obtained.

在20℃下保存24h和25℃下保存14h时,难以获得形态正常的GV期卵;体外受精获得的2细胞期胚经体外培养,总体上有64%的胚胎发育至扩张囊胚,未见有保存温度和保存时间的显著影响,且利用在15℃保存8h后的GV卵获得2细胞期胚的移植获得正常新生小鼠。

Andrew Bridge has won three Tony awards for his lighting designs: Fosse (also Ovations award, Los Angeles); Sunset Boulevard (also Ovationa award, Los Angeles) and for The Phantom of the Opera (also Drama Desk and Outer Critics' awards, New York; Dora Mavor award, Canada, and Los Angeles Critics' award). The Phantom of the Opera has now been seen in Britain, USA, Canada, Mexico, Japan, Australia, Austria,, Belgium, Germany, the Netherlands, Sweden and Switzerland.

安德鲁·布里奇已经获得了三项灯光设计的托尼奖,分别为《福斯秀》(该剧还获得洛杉机受欢迎奖)、《日落大道》(该剧还获得洛杉机受欢迎奖)、《歌剧院幽灵》(获得纽约戏剧艺术和场外评论大奖,加拿大多拉·玛沃大奖和洛杉机评论大奖),其中《歌剧院幽灵》在英国、美国、加拿大、墨西哥、日本、澳大利亚、奥地利、比利时、德国、荷兰、瑞典、瑞士先后上演。

Factors that could affect results include the ability to market and sell products; changes in relationships with strategic partners and reliance on strategic partners for the performance of critical activities under collaborative arrangements; failure of distributors or other customers to meet purchase forecasts or minimum purchase requirements for our products; impact of competitors, competing products and technology changes; ability to develop, commercialize and market new products; market acceptance of oral fluid testing or other new products or technology; changes in market acceptance based on product performance; continued bulk purchases by customers, including governmental agencies, and the ability to fully deploy those purchases in a timely manner; ability to fund research and development and other products and operations; ability to obtain and maintain new or existing product distribution channels; reliance on sole supply sources for critical product components; availability of related products produced by third parties; ability to obtain, and timing and cost of obtaining, necessary regulatory approval for new products or new indications or applications for existing products; ability to comply with applicable regulatory requirements; history of losses and ability to achieve sustained profitability; volatility of our stock price; uncertainty relating to patent protection and potential patent infringement claims; uncertainty and costs of litigation relating to patents and other intellectual property; availability of licenses to patents or other technology; ability to enter into international manufacturing agreements; obstacles to international marketing and manufacturing of products; ability to sell products internationally; loss or impairment of sources of capital; ability to meet financial covenants in agreements with financial institutions; ability to retain qualified personnel; exposure to product liability, patent infringement, and other types of litigation; changes in international, federal or state laws and regulations; customer consolidations and inventory practices; equipment failures and ability to obtain needed raw materials and components; the impact of terrorist attacks and civil unrest; ability to complete consolidation or restructuring activities; ability to identify, complete and realize the full benefits of potential acquisitions; and general political, business and economic conditions.

可能影响结果的因素包括营销以及出售产品的能力;与战略合作伙伴关系的变化以及根据合作协议对战略合作伙伴执行关键活动的依赖;经销商或者其他顾客未达到我方产品的采购预测或者最低采购要求;竞争对手、竞争产品以及技术变化的影响;新产品开发、商业化以及营销的能力;市场对唾液检测、其他新产品或者技术的接收程度、基于产品效果所造成的市场接收的变化;顾客、包括政府机构的持续大宗采购以及按时完成采购的能力;资助研究、开发以及其他产品和活动的能力;获得并维持新的或者现有的产品经销渠道的能力;关键产品部件对单一供应源的依赖;第三方生产的相关产品的供应情况;新产品或者现有产品的新疗效获得必要的管制批准的能力、时间以及费用;遵守适用的管制要求的能力;亏损的历史以及实现可持续盈利的能力;股票价格的波动;与专利保护和潜在的专利侵权诉讼有关的不确定性;与专利以及其他知识产权有关的不确定性以及诉讼费用;专利以及其他技术授权的提供情况;签订国际性制造协议的能力;产品进行国际营销以及制造的障碍;国际性销售产品的能力;资本来源的损失或者减少;履行与财务机构之间协议的能力;获得合格人员的能力;产品责任、专利侵权以及其他诉讼的风险;国际、联邦或者州的法律、法规的变化;顾客合并以及存货实践;设备故障以及获得所需原材料和部件的能力;恐怖主义袭击以及内乱的影响;完成合并或者重组活动的能力;识别、完成以及实现潜在收购的全部利益的能力;一般性政治、商业、经济形势等。

The following types of licensing practices have been found to be contrary to US antitrust law: Requiring a licensee to purchase separate unpatented products as a condition of obtaining the license for a patented product; Requiring mandatory package licensing; Forming a cartel through exclusive cross-licensing; Using a patent, trademark or trade secret as the basis for an agreement that restrains where, how or to whom a patented product is disposed of in the US after the licenser or licensee has sold the product to an independent purchaser; Agreeing in relation to a license that no further licenses will be granted to a third party without the licensee's consent; Requiring a licensee to adhere to any specified or minimum price for the licensee's sale of the licensed products; Insisting as a condition of the license that the licensee pay royalties in an amount not reasonably related to the licensee's sale of products covered by the licensed rights; Attempting to enforce a patent license or collect a royalty beyond the term of the patent (the same reasoning could apply to attempts to enforce or collect royalties on a license based on know-how that has entered the public domain); and Requiring a licensee to agree in advance to grant back to the licenser title or an exclusive license on any new patents or trade secrets related to the licensed technology rights that the licensee may obtain or develop.

下列许可行为被认为违反了美国反垄断法:●要求被许可方购买另外非专利产品作为获得某一专利产品许可的条件;以强制性一揽子方式提供许可;通过独占交叉许可组建一个企业联盟;利用一项专利,商标或商业秘密作为协议的基础,在许可方或被许可方将某一专利产品卖给一位独立买家后,限制该产品在美国的处理地点,方式和交易对象;规定某一许可在未获被许可方同意的情况下,不再向任何第三方发放许可;要求被许可方在销售其获得许可的产品时遵守特定的或最低价格;对于销售受许可权利保护的产品,要求被许可方支付不合理的特许权使用费,并以此作为获得许可的条件;试图超出专利范围强加一项专利许可或对其收取特许权使用费(同一理由可适用于试图根据已进入公共领域的技术秘密强加一项专利许可或对其收取特许权使用费);以及对于被许可方可能获得或拥有的与被许可技术权利相关的任何新专利或商业秘密,要求被许可方事先同意向许可方回授有关权利或专属许可。

Yall already know what it is and if you don't Then the great Mannie Fresh will enlighten you Ladies and gentlemen, pimps and playas Half ass rappers and true rhyme sayers This is the Carter, so hold onto your teenage daughter Because this is the Carter, a lot more rich and a whole lot smarter Wayne in ya brain young Carter Wayne in ya brain young Carter Who am I, young wizzle fa shizzle Flow sicker than a third floor in hospitals My charm starburst, my watch skittles I'm hot sizzle, what up hot bizzle Holla at a playa, I'm sorta like BD Yall can't stop my dribble, or block the lay-ups Soft top the Coupe, mami pop her too Don't chase the feelin baby girl let it come to you Remove the ceiling on the girl now she comfortable I'ma world class G mami fuck wit dude I got Lazy-Boy bank that's comfort low I'ma offense coordinator run the shoe You watch your grill what you ought to do Fore' your ribs get barbecued And my ribs is showin I say I'm starving dude This, this, this, this, this is the Carter dude What up Reel, c'mon I'm the Birdman Birdman Jr. bitch I be high in the sky shit on you and yo bitch I be flyer than your guy nigga lose the prick Now if ya follow everything just grab a wing And we could sssssshhhhhhhh All the way down to the mall and bet I buy it all Young Mar' picture the broad lick All the way down to the balls then she wipe it off If getting money is wrong I ain't right at all I young money a car I just write it off I'm the boss of my own shit, I'm my own click I would say fuck ya but go and get ya own dick Get ya game up boy hustle harder This, this, this, this, this, this, this, this, this Get ya game up boy hustle harder This, this, this, this, this, this is the Carter, yea And yall better blame Baby Cuz he the muthufucka that made me, and I'm crazy And yall ask why I never left the navy Cuz he the muthufucka that pay me, is you crazy Young, young, young wizzle baby Give you yo issue, me don't wanna kiss you No, no, no me no want no bullshit So, so stop baller blockin cuz a nigga hood rich And I floss everyday because I could bitch So holla at ya boy cuz I'm still on some hood shit Like, like, like whats really good wit ya mom or your lil boy And your sister lookin good shit, shit I'm still Apple Eagle weasel 1-7 Hollygrove never ever ever leave you I got yall waitin on my sequel This, this, this, this, this, this is the Carter people

yall已经知道它是什么,以及如果你不然后伟大mannie新鲜会启发你女士们,先生们,皮条客和普拉亚斯半驴说唱和真正的押韵sayers 这是卡特,所以举行到您的十几岁的女儿因为这是卡特,有很多更丰富的和整体堑,长一智韦恩在亚脑青少年卡特韦恩在亚脑青少年卡特我是谁,年轻wizzle发shizzle 流病加重了超过三分之一的楼在医院我的魅力starburst ,我的观赏skittles 我的热点sizzle ,有什么了热bizzle holla在playa ,我sorta一样,屋宇署 yall不能阻止我的运球,或阻止裁员的UPS 软顶的跑车, mami弹出她太不追赶feelin女婴,让它到你们这里来删除的上限,该名女童,现在她舒适我是世界一流的克mami他妈的束手无策dude 我懒惰-男童银行的舒适度低我的进攻协调员运行擦鞋您看您的烧烤什么,你应该做的前列'你的肋骨,获得烧烤和我的肋骨是showin我说我挨饿, dude 这,这,这,这,这是卡特dude 什么了轴, c'mon 我是birdman birdman小荡妇我高,在天空中shit外围对你和摇摇荡妇我比你的传单家伙nigga失去刺现在,如果亚后续的一切只是抢到的一翼我们可以sssssshhhhhhhh 所有的方式到商场和打赌,我买它的所有年轻三月'图片广大舔所有的方式到球,然后她擦拭它关闭如果得到的钱是错误的I是不正确的,在所有 i年轻的钱买车,我只是将它写小康我的老板,我自己的shit外围,我自己的点击我会说他妈的亚,但去获得亚自己的迪克获得亚的游戏了男童hustle更难这,这,这,这,这,这,这,这,这获得亚的游戏了男童hustle更难这,这,这,这,这,这是卡特,雅和yall更好地归咎于婴儿 cuz他该muthufucka使我,我疯了和yall问,为什么我从来没有离开海军 cuz他该muthufucka薪酬,我是你疯了青少年,青少年,青年wizzle婴儿给你呦问题,我不想亲吻你不,不,不,我没有想没有布尔什特所以,所以停止球员blockin cuz一nigga遮光罩丰富我每天用牙线,因为我可以荡妇因此, holla在亚男童cuz我仍然对一些遮光罩shit外围像,像,像什么真正好的束手无策亚妈妈或您的莉儿男童和您的妹妹来查找良好shit外围, shit外围我还是苹果鹰weasel 1月7日hollygrove以往任何时候都从未离开你我得到yall waitin对我的续集这,这,这,这,这,这是卡特人

"Sweep the board"=be completely successful 获得全胜,赢得全部的荣誉和奖品 The boy was clever and diligent, he won scholarship after scholarship and indeed he swept the board.

这个孩子既聪明又用功,他获得一次又一次奖学金,简直就是获得了全胜。

Results showed 172 DH plants in 2006, 230 DH plants in 2007 were obtained, 275 plants had been domesticated in 2008.Some DH regeneration plants could transform into leaf mutants. From 2006 to 2007, 8 wrinkledness leaf mutants were gotten. The highest rate of getting mutants was 2.29 %. 2 mutant plants had gotten seeds. The leaf mutants mainly showed wrinkledness in the surface of preceding leaves at early stage. Then the wrinkle leaves could transfer to normal ones and some also keep the wrinkledness later with their growth.

结果表明,CGMCC№2553具有胚胎发生能力,2006年获得172株DH再生植株,2007年获得230株,2008年驯化275株;定植到日光温室中的DH再生植株有些会转变为叶片突变体,2006~2007年共获得8株叶片皱缩突变体,最高获得率为2.29%;目前已获得2份突变体的自交种子;叶片突变体主要表现:生长初期叶片表面皱缩呈瘤状,随着生长的进行,一些皱缩的叶片转变为正常,有的则依然皱缩。

In this foundation, this paper brings up the five principles for implementation of experiential study in junior high Chinese (student-centered principle, situation-embraced principle, experience and understanding combined principle, learning strategy guided principle and joyful and harmonious principle) and eight implementation strategies of experiential study in junior high Chinese (creating atmosphere of experience through aesthetic reading, building touched experience through situation perception, obtaining implicated experience through association, obtaining situational experience through simulated performance, receiving role experience through empathized dialogue, acquiring deep experience through cooperative inquiry, obtaining creative experience through conversation with teacher and acquiring promoted experience through outward practice).

在此基础上,本文提出初中语文体验性学习实施的五大原则(学生主体原则、情境融入原则、体验理解相结合原则、学习策略指导原则、愉悦和谐原则)和初中语文体验性学习的八大实施策略(通过审美朗读营造体验氛围、通过情境感知生成感动体验、通过联想获得意蕴体验、通过模拟表演获得情境体验、通过移情对话获得角色体验、通过合作探究来获得深度体验、通过与教师对话获得创造性体验、通过拓展实践获得提升性体验)。

S ee How to Work with Herbs and Essential Oils for more information and some lovely homemade spritzers, creams and toothpaste recipes; also From the Gum and Eucalyptus Trees for recipes for homemade skin lotions; and From the Flowering Plants Trees and Bees for a new regeneration oil recipe that feeds the skin and is also useful unto ascension; and From the Sweet Smelling Plumaria Blossoms for homemade perfumes and non-toxic cleaning substances.

请看如何与药草与精油一起工作,来获得更多信息,并获得一些可爱的自制喷雾、护肤霜和牙膏配方;也请看来自橡胶树与桉树,获得自制护肤乳液的配方;来自有花植物树木和蜜蜂,来获得一份新的再生油配方,那是滋养皮肤并对提升有益的;来自香甜的鸡蛋花,获得自制香水及无毒的家居清洁剂配方。

Outlined in the Beijing Olympic Games, at 13:30 on August 13 Switzerland and France than was Ankan Qiela La Road Cycling Championship. Ankan Qiela La France in the World Championships: 2004 Bardolino, Italy was eighth, 2005 Madrid, Spain, was third, 2006 Salzburg, Austria received first place in 2007 at Stuttgart, Germany was the first ; France 2005 cycling sai Laise Skopje was seventh; Flanders Cycling Grand Prix E3 second place; Belgium Harelbeke 2007 Road Cycling sai second place; Florence - Bicycle sai Pistoia, Italy Paper 2004 Si Tuoya fourth; Ghent - Victoria Buddhist roots bike sai fourth; Road Cycling Events, the Belgian peacekeepers Buddhist roots fourth in 2006 Belgian peacekeepers were sixth Buddhist roots; HEW Classic Bicycle Germany 2005 Hamburg Road Cycling sai fourth in the 2006 German Beer, Luke second Challenge; Milan - San Remo Road Cycling sai second place; San Remo, Italy, the 2008 Road Cycling sai was the first; Monte Siena, Italy 2008 Paschi Eroica Road Cycling sai was the first Swiss national championships, in 2008 Switzerland was the first Messen; Stettlen 2007 Switzerland was the first in 2006 was the first Ruishiboning root 2005 Switzerland was the first Hagendorn; Highway Cycling Merit 2007 Rui Shibu Kruger was second in Paris - Roubaix bicycle sai , 2006 France was the first Roubaix; Roubaix France was second in 2008; 2005 Roubaix France was eighth; Denmark Post bike sai The 2006 final ranking was the first Danish Frederiksberg; ring The 2006 Belgian Flanders Cycling Road Cycling sai Meerbeke was sixth; Etruscan - Adriatic bike sai , 2008 Italy's Benetton and child care - Badelt-care was the first in the Central bike sai Luxembourg, Luxembourg Central Di Jixi 2005, was second; bike sai Central California, the United States in 2008 in Pasadena, California, was fourth ; Athens, Greece in 2004 was the 10th individual time trial; Olympics

瑞士法比安坎切拉拉在世界锦标赛:2004 意大利巴多利诺上获得第八名,2005 西班牙马德里上获得第三名,2006 奥地利萨尔茨堡上获得第一名,2007 德国斯图加特上获得第一名;2005 法国莱塞比耶自行车赛获得第七名;佛兰德E3自行车大奖赛获得第二名;2007 比利时哈勒尔贝克公路自行车赛获得第二名;佛罗伦萨-皮斯托亚自行车赛,2004 意大利皮斯托亚获得第四名;根特-维佛根自行车赛获得第四名;公路自行车赛 2005 比利时维佛根获得第四名;2006 比利时维佛根获得第六名;HEW自行车经典赛 2005 德国汉堡公路自行车赛第四名;2006 德国比尔,路克挑战赛第二名;米兰-圣雷莫公路自行车赛获得第二名;2008 意大利圣雷莫公路自行车赛获得第一名;2008 意大利锡耶纳 Monte Paschi Eroica 公路自行车赛获得第一名;瑞士全国锦标赛,在2008 瑞士Messen 获得第一名;2007 瑞士Stettlen 获得第一名;2006 瑞士伯宁根获得第一名;2005 瑞士Hagendorn获得第一名;公路自行车赛 2007 瑞士布鲁格获得第二名;巴黎-鲁贝自行车赛,2006 法国鲁贝获得第一名;2008 法国鲁贝获得第二名;2005 法国鲁贝获得第八名;丹麦邮政自行车赛决赛排名赛 2006 丹麦腓特烈斯贝获得第一名;环佛兰德自行车赛 2006 比利时Meerbeke 公路自行车赛获得第六名;伊特鲁里亚-亚得里亚海自行车赛,2008 意大利圣贝内代托-德尔特龙托获得第一名;在环卢森堡自行车赛,2005 环卢森堡迪基希,获得第二名;环加利福尼亚自行车赛,在2008 美国加利福尼亚帕萨迪纳,获得第四名;2004 希腊雅典个人计时赛获得第十名;奥运会 2008 北京奥运会男子公路个人赛季军。

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The physiology of the skin machine body for the sake of the environment in the maintenance stability, but eject a body metabolism creation of metabolism thing BE'grease', summer perspire many various skin disease light or good, autumn perspire little metabolism thing metabolism don't go out, various skin disease made, this be the skin machine body of various burning disease.

皮肤机体的生理为了维持内环境稳定,而排出身体代谢产生的代谢物是'油泥',夏天出汗多各种皮肤病就轻或好了,秋天出汗少代谢物代谢不出去,各种皮肤病就犯了,这就是皮肤机体的各种炎症。

If I had anything tender in me, I shot it dead.

如果我有什么招标的话,我枪死亡。

The argumentation way in which this literary grace using is based on color painting, setting out from two angles separately " color"、" ink and wash", making criticizition in texts of Chinese ancient drawing history; analyzing how "color" painting was on the way from ripe to losing; emphatically analyzing the reason of losing in color center, that is to say the reason of "the change of ink and wash ", and its reconstructional way of combination, development with "ink and wash" it was "replaced" by the afterward manner. In a word, the developing "replaced"by the afterward manner.

本文所采用的论述方式是:立足于色彩绘画,分别从&色彩&、&水墨&两个角度出发,先对它们在中国古代画史论著中作一考证;分析隋唐时期&色彩&绘画是如何从体制的成熟、完备而走上&失落&的道路;着重分析色彩绘画中心&失落&亦即&水墨之变&的原由;及其在被水墨&替代&后与&水墨&结合、衍变的重建之途。