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At present, research has been carried on about resources of green lacewing and its biology, ecology, ethology of common species, and reproductive technology on a large scale, natural feed and artificial feed as well. However, research on reproductive physiology respect of green lacewing now is less and hasn"t gone too far. This article has carried on certain studies on this respect, aiming at offering theoretical direction for giving play to green lacewing"s reproductive latent, raising artificial reproductive efficiency and field effective protection and utilization.

目前我国对草蛉的资源和常见种的生物学、生态学、行为学以及大规模繁殖技术做了广泛的研究,但目前草蛉的生殖生理方面研究的较少,认识尚不深入,本文就这方面进行了一些研究,旨在为发挥大草蛉的生殖潜能、提高人工繁殖效率及田间有效保护利用等提供理论指导。

Chrysopa septempunctata was fed with two foodstuffs, aphids and artificial ovum Trichogramma's pupas. The results indicated that survival time of female worms which intook aphids was obviously longer compared with those which intook artificial ovum Trichogramma's pupas, and their ovum quantity obviously rose. But artificial ovum Trichogramma's pupas can be used as supplementary foodstuff to raise green lacewing because of its factory production.

用蚜虫和人工卵赤眼蜂蛹两种食料来饲养大草蛉的结果表明,喂食蚜虫的大草蛉雌成虫存活时间明显长于喂食人工卵赤眼蜂蛹的大草蛉,产卵量亦明显提高,但人工卵赤眼蜂蛹已经实现了工厂化生产,可以作为饲养草蛉的补充食料。

Chrysopa septempunctata was fed with two foodstuffs, aphids and artificial ovum Trichogramma"s pupas. The results indicated that survival time of female worms which intook aphids was obviously longer compared with those which intook artificial ovum Trichogramma"s pupas, and their ovum quantity obviously rose. But artificial ovum Trichogrammas pupas can be used as supplementary foodstuff to raise green lacewing because of its factory production.

用蚜虫和人工卵赤眼蜂蛹两种食料来饲养大草蛉的结果表明,喂食蚜虫的大草蛉雌成虫存活时间明显长于喂食人工卵赤眼蜂蛹的大草蛉,产卵量亦明显提高,但人工卵赤眼蜂蛹已经实现了工厂化生产,可以作为饲养草蛉的补充食料。

The mechanism of photosensitivity and phototaxis was studied with optical andelectrophysiological method. The results are as follows:1 among three kinds of Chysopa peral. of the compound eyes show little daily change in Chrysopa sinica Tjedar .The model and color of psesudpopupil show significant daily change in Chrysopa septempzhbybimctata Wesmacl and Chrysopa formosa Brauer, color and diameter of the psesudpopupil of the compound eyes in Chrysopa septempunctata Wesmacl make rapid change at noon, afternoon ,night are similar ,but make little change in the morning and the next morning. Diameter of psesudpopupil of compound eyes is the biggest in the afternoon, which of Chrysopa formosa Brauer, is the biggest at noon.

本文应用光学及电生理学方法对棉铃虫优势种天敌—中华草蛉、大草蛉和丽草蛉3种草蛉的感光和趋光机制进行了研究,主要研究结果如下: 1、中华草蛉、大草蛉、丽草蛉复眼伪瞳孔大小、颜色昼夜间有节律性变化,且种间具有不同的变化模式。3种草蛉中,中华草蛉复伪瞳孔大小昼夜节律变化不明显,其模式和颜色上亦基本无变化,中间为一小黑点,其外围是绿色,最外层为红色,形状为正六边形:大草蛉、丽草蛉的复眼伪瞳孔大小昼夜间不同,大草蛉的复眼伪瞳孔在中午、下午、晚上变大,早晨和次日早晨基本一致,但次日早晨稍小些。

Chrysopa sinica is major natural enemy of thrips. Functional response and searching rate of C. sinica larvae on Frankliniellaoccidentalis nymphs were studied in laboratory in order to learn the control efficiency of the C. sinica on F.occidentalis.

中华草蛉是蓟马的重要天敌,为探明其对西花蓟马的控制效能,在室内开展了中华草蛉幼虫对西花蓟马若虫的捕食功能反应与搜寻效应研究。

The main conclusions are as follows:The vitellin of Chrysopa septempunctata was extracted with 0.4mol/L NaCl and then precipitated in cold distilled water. Ion exchange chromatography and proteic gel recovery were used to purify it further. SDS-PAGE analysis shows that the vitellin is composed of two subunits, the large one 165kDa and the small one 46kDa.Then automatic amino acid analyzer was used to analyse amino acid composition of vitellin.

主要结论如下:用蒸馏水沉淀法对大草蛉的卵黄蛋白进行了初步纯化,而后又采用离子交换层析和蛋白胶回收的方法对卵黄蛋白进行了进一步的纯化;通过SDS-PAGE分析表明,大草蛉的卵黄蛋白由大165kDa和小46kDa两个亚基组成;用氨基酸自动分析仪对纯化后的卵黄蛋白进行了氨基酸组分分析。

The titer variation to juvenile hormone of female worms was detected by GC-MS system, from which there was an obvious correlation between variation regularity to juvenile hormone and vitellogensis's regularity in Chrysopa septempunctata.

通过GC-MS系统测定了大草蛉雌虫体内保幼激素Ⅲ滴度的变化,从中可以看出大草蛉体内保幼激素滴度的变化与卵黄蛋白发生存在明显的相关性。

The titer variation to juvenile hormone of female worms was detected by GC-MS system, from which there was an obvious correlation between variation regularity to juvenile hormone and vitellogensiss regularity in Chrysopa septempunctata.

通过GC-MS系统测定了大草蛉雌虫体内保幼激素Ⅲ滴度的变化,从中可以看出大草蛉体内保幼激素滴度的变化与卵黄蛋白发生存在明显的相关性。

The whole curve appears near S shape curve which revealed that the compound eyes of green lacewinng have strong adapting ability to light stimuli.4 Relation of the dark-adapted time and ERG value indicates that time of day eyes turning to night eyes is not similar in different kind of species. The turn of compound eye from day eyes to night eyes need about 120min in Chrysopa sinica Tjedar,, but is not accord to in different unit the other two species.

以ERG值为指标,在—定时间内,暗适应时间长短影响草蛉复眼的ERG振幅的大小,不同种革蛉其ERG值达稳定所需时间是不—致的,中华草蛉一般都需要120m,ERG值才能达到稳定,而大草蛉、丽草蛉不同个体ERG值达到稳定的时间不完全一致,大草蛉ERG值一般需60-90m达稳定,个别在120m左右达稳定,丽草蛉ERG值一般在60m左右达到稳定。

The result suggested the presence of three typesreceptors at least in the compound eye of Chrysopa sinica Tjedar and Chrysopa50septempunctata Wesmacl, but four type receptor in Chrysopaformosa Brauer.3 The intensity make effect on ERGs value .The result indicate that the stronger the luminance, the higher the ERG value in a certain intensity range of monochromatic and white light stimuli,but the rate of improvement make creeping until the extent to intensity.

光强度对3种草蛉ERG值有影响,—定光强度范围内,草蛉复眼的ERG值皆随白光刺激光强度的增加而增大,至一定光强度时增加变缓,(但是在光强度为log=0时,并没有出现—个反应平台),呈现出—种近似线型的式样,表明:草蛉成虫复眼具有较强的光强度自调节和耐光能力。

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