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These two series of probes differ in their mechanism of action. The first series exploits the concept of suicide substrate. When the probe is hydrolyzed by the target sulfatase, it will release an intermediate that undergoes elimination to form a reactive quinone methide which in turn reacts with nearby nucleophiles. The second series belongs to an electrophilic reagent which utilizes a cyclic sulfamate moiety serving both as the recognition unit and the trapping device.

这二系列标示分子的主要差异在於其标示机制,第一系列是源自於自杀性受质的概念,利用水解后的中间体会生成高反应性的quinone methide,来达到标示的效果;第二系列则是属於亲核性试剂型,采用环状胺基磺酸衍生物做为辨识端及捕捉机制,预期在其进入酵素的受质结合区后能与酵素催化中心形成共价键结。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板,用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF,其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体,构建的重组质粒命名为pET-30a-PN;将pET-30a-PN转化到大肠杆菌BL21 (DF3)中,在IPTG诱导下进行表达;SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白,重组蛋白以包涵体形式存在;薄层扫描结果表明表达产物占菌体总蛋白的30.5%;Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应,说明该重组蛋白具有免疫学活性。

The U.S. Food and Drug Administration's Oncologic Drugs Advisory Committee voted 10 to 0 that the objective responses seen in the studies were strong enough to serve as a surrogate that was "reasonably likely" to predict clinical benefit as a treatment for patients with previously treated glioblastoma.

食品与药品管理局的肿瘤药物咨询委员会以10:0的投票支持该研究,他们认为该研究有足够的事实依据,证明其可以作为过去曾经接受过治疗的多形性恶性胶质瘤患者的一种&合理的&替代治疗方法,可以预期患者能从该治疗当中获益。

The readiness with which men accustom themselves, as matters of course, to improvements in their condition, which, when anticipated, seemed to leave nothing more to be desired, could not be more strikingly illustrated.

与哪些人熟习,作为当然的事项准备,在他们的状况,而当预期,似乎没有留下更多的是需要改进,不能更惊人的说明。

Other things being equal, for example, we should expect pressures toward vertical integration in a market where transacting firms lack a network of personal relations that connects them or where such a network eventuates in conflict, disorder, opportunism, or malfeasance.

例如,其他的条件同样的情形下,那些参与交易的厂商缺乏一种能将它们连接起来的个人关系网络,或者这种网络是导致冲突的、无序、机会主义或者违法,我们就会预期存在进行垂直一体化的压力。

Because soil vapor extraction technology and air sparging technology mainly depend on physical mechanisms, such as basic computation of fluid dynamics, the expected results are often obtained if one can control the required variables.

本论文主要针对国内已完成整治加油站污染场址,经由场址背景资料汇整与场址采用整治方式之适切性及其成效做探讨,分析几个影响整治成果之因素,包括采样位置、污染时间、地下水位等,进一步选定常见的土壤气体抽除法及空气注入法成功案例评析,而依据各场址整治流程发现,整治工法能否成功,掌握改善作业之关键点因素是最重要的部分,如场址特徵,污染物扩散情形、土壤质地,及是否适合现地整治等,由於土壤气体抽除法及空气注入法之整治方式,主要为物理性机制,牵涉到一些基本流体力学的计算,只要掌握足够之变数因子,利用推导方式,设置适合之整治井数量,通常应能获得预期之成效,但前提仍是现地场址的特性调查须完整,如此推导之结果才具有足够之可信度,由於国内整治计画及成果报告内容中,针对污染场址所施行工法的设计参数资料大多仅略述,仅将重点放在整治后采样之数据上,因此往往其整治成效在理论推导上较缺乏支持。

The researchmethodology system is as follows:The variables that can be measured and have significance to stock relative intrinsic value are industry characteristic, profitability,development potential,qulity of earing and asset, the expandable potential of capital stock,the ability to pay off the debt,asset manging ability and the scale of the capital stock;(2)When the MFPM is established, the original variables should be disposed beforehand;(3)As for the research objects.we should value synthesizationly the achievement of the listed,and take the No.

本章所建立的研究方法体系为:(1)上市公司个股基本面因素中可以量化,并且对股票相对内在价值有重要影响的主要有行业特性、盈利能力、成长性、盈利质量及资产质量、股本扩张潜力、偿债能力、资产运营效率和股本规模大小等方面;(2)在建立MFPM模型时,原始指标值是不能直接用于模型计算的,必须对数据进行预处理后才能用于模型中:(3)在确定研究对象时,需要对上市公司经营绩效进行综合评价,在剔除主要靠未来业绩预期定价的第Ⅳ类股后,把第Ⅰ、Ⅱ和Ⅲ殴作为模型研究对象;(4)在选择多元回归模型时,由于原始自变量由于存在错综复杂的关系,尽管对原始自变量进行过预处理,但自变量指标依然会存在一定的多重共线性,为此在建立MFPM模型时,须采用逐步回归模型;(5)在检验MFPM模型有效性时,须从统计意义和投资实战意义两个方面进行。

Spontaneously and immediately, the system also can control the swing of the plate following the input of the handle and reach the desired position within 0.3 second.

依据这样的能力,我们设计了两个系统功能:一、智慧型人机互动球平台游戏,包含「人机互动」(如:X轴自动Y轴手动或X轴手动Y轴自动)、「人与人互动」等,而此部份已证实能达到预期之目标;二、球位置的平衡控制,此是要控制球停止於所欲的位置,由於所需的控制法则相当复杂,本研究只进行至可验证架构的可行性,而控制法则的研究,则待未来的学弟来进行。

Second,it promotes the KMV model and calculates the Black-Scholes formula,the distance to default and the expected default frequency when the company\s assets on the Jump-diffusion process,it expands the application of the model.

扩散过程时相对应的Black-Scholes 公式和违约距离和预期违约率,具有一定的新意和应用价值,但摘要不能较好地反映文章的内容和结论,英文摘要包括单位的英译都存在明显的语法和用词问题。

An early look at a version with only a rough sound mix confirms some expectations — the film is dark, desaturated, and visually stunning — and yields some revelations: First, Johnny Depp can actually sing, and second,the movie's got more spurting blood than a season's worth of E.R.

早期粗略混音的一个版本证实了大家的预期——黑暗,复杂,有视觉冲击——同时也揭示了一些内幕:首先,强尼·戴普确实能唱好歌;第二,影片中使用的假血比一整季《急诊室的故事》使用的还多。

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In her eyes, because that is the doorway toher heart * the place where love resides.

女人的漂亮必須從她的眼睛中去看,因為那是她心靈的窗戶和愛居住的地方

I will send some postcards to you.

我会向你发送一些明信片。

Of aesthetics and the definiteness of its content and leads to its immaturity and decline .

它的不确定,直接影响美学学科定位乃至研究内容的确定,导致美学学科的不成熟和衰微。