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Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P<0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略,结合启动子预测结果,分别构建了8个ACL基因和19个IDH3β基因启动子区重组子,将其转染猪PK15细胞系,并利用荧光素酶双报告基因系统检测了它们的活性,结果表明:在所构建的猪ACL基因5侧翼序列的8个重组子中,除pGL-ACL27和pGL-ACL15外,其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P<0.01),表明它们均具有启动子活性,pGL-ACL919活性最强,到pGL-ACL679活性下降了将近3倍,说明从-919bp到-679bp区域可能存在负的调控位点,从pGL-ACL621到pGL-ACL73活性有小幅度下降,到pGL-ACL27活性降到与阴性对照水平无显著差异,表明维持该启动子的基本活性区域位于-73bp到+77bp之间;IDH3β基因启动子活性开始于pGL-IB82,然后虽然有些波动,但是活性一直下降直到-164bp处,之后活性又开始升高,直到-279bp处活性达到最高,这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间,从-82到-279之间的179bp区域具有最高的启动子活性。

If we physically knew that five skandhas are empty and vanity are form, feeling, perception, impulse and consciousness, which are unworthy of seeking by time and energy, as long as meanwhile we could maintain basic living condition and practice hard in our limited life, then we would discover that it is unnecessary for us to toil all day as horse and that it is not so expensive to maintain life's basic elements.

如果我们真正地了解到五薀皆空,色受想行识俱为虚妄,不值得我们花太多时间及精力去追求,同时,我们只要能维持基本的生活就能保住性命,并且在有限的时空中努力修行的话;那麼,从中我们就会发现,维持生命的基本要素其实并不是那麼的昂贵,并且也不需要我们累得像一头牛似的整天劳动个不停。

In nature, forest is resource-base, gene-base and power-base which take on the most perfect function. It is very important to maintain terrene environment and ecologic balance. Forest, as the provider of natural resource which is necessary to mankind development, take on significance to social sustainable development.

森林是自然界功能最完善的资源库、基因库、蓄水库和能源库,对维持陆地生态环境,维持生态平衡起着重要的作用,同时森林作为人类发展必不可少的重要自然资源提供者,对社会经济的可持续发展具有极其重要的意义。

The natural rate of unemployment is the lowest rate to maintain the unchange of inflation , it means the hightes maintainable rate of employment .

自然失业率是维持通货膨胀不变的最低失业率,他代表最高的可以被维持住的就业水平。

As the upkeep step begins, any abilities that trigger at the beginning of that upkeep step and any abilities that triggered during the turn's untap step go on the stack.

维持步骤开始时,在该回合之重置步骤中触发的异能,以及在维持步骤开始时触发的异能均进入堆叠。

The results of the determinating the activity of the CA in vitro showed that the CA of the water—fed individuals were in activing statics after eclosion. Because of the apperence of the Vg in haemolymph and the deposition of the vitellin in oocytes in the unfed females, it was shown that the CA in such individuals were in activiting statics, too. Again because in the water—fed females which did not lack the feeding action the activity of the CA showed dropping tendency gradually still, it could be said that the activiting of the CA was possibly effected by the nutrition but it was never related to the adult feeding action. In another respect, the brain of the female adult had not significant effect on the activiting CA. The oocytes could depositing vitellin in individuals removed brain tissue on the day of eclosion, which showed that the activting of CA was not concerned with brain.

CA活性的体外测定说明饲水个体的CA同样具有活性,不取食个体血淋巴中Vg的存在以及卵母细胞中卵黄的沉积表明,在不取食的个体中CA同样具有活性,所以CA的活化与成虫期的取食动作无关,与成虫是否取食花蜜也无太多的联系,但是CA活性的维持可能受成虫期营养的影响,如果以卵母细胞发育和CA体积大小作为CA活性的指标,则饲水个体CA的活性在后期受到了影响,说明饲水不能替代取食蜂蜜来维持成虫期CA的活性;成虫期脑对CA的活化无明显作用,羽化当日摘除脑的个体,其卵母细胞仍然可以沉积卵黄,说明CA的活化与脑无关,而是受某种不确定的因子的影响;用抗JH物质—早熟素—Ⅱ以10ug/虫的剂量在颈部点滴处理雌虫,对Vg的发生和卵巢的发育具有一定的迟缓作用,其原因有待于进一步的研究。

The conclusion of three burning methods mentioned above during the heat storage period is that the first method is the best one, which is propitious to keep higher blast temperature because of the heat exchange in the middle and blow of hot blast stove has been aggrandized through convection heat transfer caused by the added exhausted air quantity and increased flow rate.

以上三种燃烧制度,在蓄热期:第1种因废气量增大,流速加快,利于对流传热,强化了热风炉中下部的热交换,利于维持较高风温,第2种因废气量减少,不利于传热和热交换的强化,不利于维持较高风温,第3种由于废气大量减少,流速降低,传热减缓,蓄热量较少,不利于提高风温。

The results showed that the metabolizable energy for maintenanceand erude protein for maintenace of the clucks on the average were 925.82KJ/W0.75/d,10.200.7g/W0.75/d in 1st to 21th day of age and 884.52KJ/W0.75d.8.23g/W0.75/d in 22th to 49th day of age,respectively.

结果表明,樱桃谷肉鸭的平均维持代谢能和维持粗蛋白的需要量是:1~21日龄MEm=925.8 KJ/W~(0.75)/d,CPm=10.2g/W~(0.75)/d:21~49日龄MEm=884.5 KJ/W~(0.75)/d,CPm=8.2g/W~(0.75)/d。

About 78 percent of marriages lasted five years or more, compared with less than 30 percent of what the CDC called cohabiting unions, or couples living together outside marriage.

美国约78%的婚姻关系可维持五年或五年以上,而相比这下,只有不到30%被该机构称为非婚同居的关系可维持这么久。

Percent of marriages can maintain 5 years or more, compared to it, less than 30 percent cohabits can last such a long time

美国约78%的婚姻关系可维持五年或五年以上,而相比这下,只有不到30%被该机构称为非婚同居的关系可维持这么久。

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推荐网络例句

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。