结合的

Under the direction of sustainable development theory, qualitative and quantitative, authentic and standardize, theorize and factuality study on sustainable developing management.

本文运用可持续发展的理论、思想，采用定性分析与定量分析相结合、实证分析与规范分析相结合、理论研究与案例分析、对策研究相结合的方法，对城市建设可持续发展管理进行了系统的分析研究。

The results suggest that CHBr3 is likely to intercalate into ctDNA and such intercalations mainly depend on the relative binding affinity of a metal ion to the bases and to the phosphate groups in DNA.

据此推断，CHBr3可能主要是通过嵌插作用与ctDNA碱基结合，金属离子对CHBr3与ctDNA结合的影响主要取决于金属离子与DNA的碱基和磷酸基团间结合的相对亲和比。

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关，一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力，FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现，目前已有很多的研究发现FIS与DNA结合的机转，然而这些实验的数据仅能显示推测性的结果，进而利用这些结果推论出FIS与DNA聚合体结构的模型，本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节，我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型，并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域，我们也分析FIS与DNA之间的氢键，并且与实验的资料作比较，最后我们计算FIS与DNA之间的结合自由能评估模拟的结果，并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

Explosive welding process is characterized in large plastic deformation and undulate interface in bonding zone.

爆炸焊接结合区产生大的塑性变形及波状结合，是爆炸焊接工艺的主要特征，也是实现良好冶金结合的必要条件。

The compensation system of first-line workers of state-own coal enterprise was redesigned under the direction of compens

结合相关薪酬理论，对国有煤炭企业一线员工的薪酬体系进行了全新设计，构建了由基本薪酬、效率薪酬、安全薪酬及福利薪酬四大部分组成的以激励为导向的&绩效薪酬体系&，详细论述了各大组成部分的设计方法和设计步骤：综合考虑企业生存状况和社会收入状况，采用以劳动定额和社会平均收入相互平衡的方法，设计出一线员工的基本薪酬模块；提出了用专家评议法确定施工难度等级指标体系的相关权重、用综合模糊评价法来确定作业难度等级、把团队人力资源要素与作业区域的施工难度状况紧密结合起来测算团队业绩，构建出体现团队业绩和贡献的效率薪酬模块；构建了以&安全抵押&制度为核心，以月度安全收入、季度安全收入、年度安全收入为框架，形成了短期、中期与长期安全收入相结合的安全薪酬模块；结合国有煤炭企业一线员工来源复杂、身份复杂、需求不同的现状，采用了员工参与性和自决性极强的&自助式&福利薪酬模式。

Skill-mental dominated event-group has characteristics what are polarizing frighten goal and fluctuating variation course. 6 Coaches and athletes must insist on the principles that is comminuting of judging accurately and intervening in good time, perspective with getting practical to combine together, timeliness and synchronous combine together, controllability and principle that substantial results combine together.

影响竞赛过程中运动员竞技能力变异的众多因素可聚合为以下四个相对独立又相互联系的子系统，即：运动员的竞技状态与相对竞技实力、参赛认知水平与比赛经验、可逆转环境和中立环境。4运动员竞技能力的参赛变异具有变异成因的多元性和多变性、变异内容体能的先导性、变异过程的可控性与非可控性、变异结果的两极性和层次性和参赛行为的心态易变性等五个方面的一般训练学特征。5体能主导类项群具有变异结果直观性、变异过程连续性和对手制约两重性等特征；技能主导类项群具有单项贡献制约性、个体变异传递性和裁判评定诱导性等特征；技战能主导类项群具有群体整合共生性、核心技战术制导性、攻防转换制约性和局盘赛点突增性等特征；技心能主导类项群具有目标恐惧极化性和变异过程波动性等特征。6教练员、运动员必须坚持准确判断和适时干预相结合、前瞻性与务实性相结合、适时性与同步性相结合、可控性与实效性相结合的原则。

The five prosthetic groups (FAD, [2Fe-2S], [4Fe-4S], [3Fe-4S] and heme group) required for electron transfer from succinate to ubiquinone were unambiguously assigned into the electron density map. Besides, we find there are some electron densities around the Qp pocket at the matrix side and we believe it represents the head structure of ubiquinone, which is proved by the inhibitor bound structure that 2-TTFA just locating at the Qp site. At the same time, we find there is the second 2-TTFA binding site, locating the inter-membrane side. This finding will change our knowledge about the electron transfer inside Complex II and the ubiquinone transfer between Complex II and Complex III, and endow a new role of Complex II in electron transfer chain.

除了对各个电子传递体（ FAD ，[2Fe-2S]，[4Fe-4S]，[3Fe-4S]以及血红素分子）进行精确定位外，我们在该结构跨膜区靠近线粒体基质一端的口袋 Qp 中，发现了一些电子密度，认为是所结合的辅酶 Q 的头部结构，这一点被与抑制剂结合的复合体的结构所证明，在该结构中， 2－ TTFA 恰好结合在口袋 Qp 中，同时，我们还发现了第二个2－ TTFA 的结合位点，位于跨膜区靠近线粒体膜间隙一端的口袋 Qd 中，这个发现具有全新的意义，将影响人们对电子在复合物 II 中传递以及辅酶 Q 在复合物 II 与 III 之间转移的认识，促使人们重新复合物 II 在线粒体呼吸链中的角色。

Relying on both dynamic and static analysis methods,both microstructure and macrostructure analysis methods,both on-line and off-line analysis methods,as well as both along-line sampling and die sampling techniques,the author of this thesis studied and developed an on-line microscopic system,the on-line rheometers and the on-line samplers which are used in single screw extruding process for the first time. Based on the analyses of a plenty of experimental results, the thesis mainly discussed following several questions: solid-bed break-up phenomenon on macroscopic scale,melting and deforming mechanism of a solid particle on small particle scale,and polymer morphology on microscopic scale.

本文作者通过把动态和静态分析相结合、微观和宏观分析相结合、在线和非在线分析相结合、沿程取样和机头出口样品分析相结合的方法，在单螺杆挤出过程的在线显微观察与摄影、在线流变测试、在线取样的大量实验分析的基础上，针对挤出过程中从宏观尺度范围的固体床破碎现象、到较小的颗粒尺度范围的固体粒子的熔融与变形、以至微观尺度范围的聚合物材料的形态结构的变化情况等有关几个的问题进行研究讨论。

The dose-dependent experiment revealed that Id1 antagonised E47 and Ets1 mediated transcriptional activation in a dose-dependent manner. With pGL3-870 as a template, site-directed mutagenesis of E-box and / or ETS-binding site was introduced into p16 promoter. E47 could not activate the transcription of p16 promoter with E-boxes mutated, and Ets1 could not activate the transcription of p16 promoter with EBS mutated. The synergy effect between E47 and Ets1 would occur only if the E-boxes and EBS were present. These facts demonstrated that E47 and Ets1 proteins were not recruited to the promoter by binding to other cis-elements or through interaction with other protein factors, however, they bound to DNA directly through respective consensus sequences in p16 promoter.

以pGL3-870为模板，构建了E-box或/和EBS点突变的p16启动子报告基因载体，将野生型和突变型的报告载体分别与pI-E47、pI-Etsl以及pI-E47+pI-Ets1共转染，发现E47和Ets1所诱导的转录激活作用依赖于启动子DNA上存在的各自的特异结合序列，二者的协同作用也只有当两个结合位点都存在时才会发生，说明E47和Ets1不是与其它作用元件或其它蛋白质因子相作用而被招募到该启动子的，它们是通过启动子上各自的特异结合位点与DNA直接结合的。

The RhoA oncogene , have been revealed in recent years, are closely related to various malignant tumours. RhoA, belonging to Rho family, is one member of the ras superfamily, whose encoding production, namely RhoA protein, pertaining to micromolecular mass GTP conjugated protein family, in GTP conjunctive active form and GDP conjunctive unreactive form. It exist in the latter form in normal cells and the switch of the two forms enables them to educe functions, paralleling"molecular switch", the diminutive G protein in activated state being able to activate the wink dromo-iter to work functions, which is in the resemblance of process during which the Ras genic mutation induces its continous activations.

RhoA是近年来新发现的与多种恶性肿瘤密切相关的癌基因，RhoA属于Rho亚家族，是ras基因超家族的一员，其编码产物RhoA蛋白属于小分子量GTP结合蛋白族，分为GTP结合的活性型和GDP结合的非活性型两类，在正常细胞内以GDP结合的非活性型存在，两种活性形式之间的转换使他们能够发挥一种类似&分子开关&的作用，活性态的小G蛋白能激活下游的信号传导通路，行使其功能。

Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事，那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来，随着部分预应力砼梁应用的推广，发现我国规范所采用的名义拉应力法的计算结果不稳定。