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Results: The normal epithelial cells were intactly lined with microvilli, and lesions on the surface of Ct infected cells were showed by SEM. The expression of actin was obvious throughout the cytoplasm of uterine epithelial cells but appeared to be most intense along the apical plasma membrane on day 4, 5 and 6 of pregnancy. On the day 7, levels of actin had increased along the apical surface. The expression of actin in the infected group was weaker than that in of the normal group.

结果:SEM示对照组子宫腔可见有微绒毛的分泌细胞;实验组腔上皮细胞表面有的可见破损,微绒毛少见;免疫组化结果显示荧光强度主要定位于腔上皮细胞胞质/胞膜,妊娠第4,5,6天沿胞质顶部分布较密集,而底部较弥散;妊娠第7天顶部强度有所增加;感染组的荧光强度均弱于相应正常组,在妊娠第5天存在显著性差异(p.05)。

Leaves 29-84 cm; petiole 7-12 cm, pilose to glabrescent, often lenticellate; rachis moderately pilose to glabrescent, occasionally velutinous; leaflets usually 6-9(-12) pairs; petiolules (2-)4-12 mm, pilose/villous to glabrescent; leaflet blades lanceolate to ovate-lanceolate, 7-14(-19.5)× 3.1-6(-7) cm, often dark grayish brown, moderately pilose to glabrescent, usually with short trichomes and club-glands apically on midveins, veins basally pilose to villous/velutinous, base symmetrical to asymmetrical, margin entire, apex acuminate to occasionally acute.

叶29-84厘米;叶柄7-12厘米,具柔毛到几无毛,通常具皮孔;轴适中具柔毛到几无毛,偶尔被短绒毛;小叶通常6-9(-12)对;小叶柄(2-)4-12毫米,具柔毛/具长柔毛到几无毛;小叶叶片披针形到卵状披针形, 7-14(-19.5)* 3.1-6(-7)厘米,经常暗灰棕色,适中具柔毛到几无毛,通常在顶部中脉上具短毛和棍状腺体,脉基部具柔毛到具长柔毛/被短绒毛,基部匀称到不对称,边缘全缘,先端渐尖到偶有锐尖。

The results showed that ACP positive reactions were mainly deposited in lysosome, nucellar cell, cellular endomembrane, mitochondriurn, and endoplasmic reticulum in four tissues of the healthy shrimp. Furthermore, ACP activity was located in microvilli, and around partly lipid droplet in liver. AKP positive reactions were mainly deposited in cell membrane, nucellar cell, lysosome. endoplasmic reticulum and cellular endomembrane in four tissues.

结果显示,在健康的对虾体内,ACP依次出现在各组织中的溶酶体、细胞核、细胞内膜、线粒体、内质网以及肝脏组织中的部分脂滴周围及微绒毛中AKP依次出现在各组织中的细胞膜、细胞核、溶酶体、内质网以及肝脏组织中的脂滴周围及微绒毛中。

The expression levels of Cx genes were compatible with the differenciation and development of placental villous cells.

Cx基因的表达与绒毛细胞的分化及绒毛的形成过程基本一致。

Leaves toward base of stem: petiole ca. 0.8-1.3 cm, tomentose, prophyll up to 1/2 as long as petiole; leaf blade ovate or narrowly ovate, 10-18 × 5-10 cm, papery, glabrous to abaxially tomentose, adaxially pilose along veins, base cordate, oblique, apex acute or acute-acuminate; veins 9, apical pair arising 1-3 cm above base, others basal; reticulate veins conspicuous.

约的对于scircapetiole的基部的叶0.8-1.3厘米,被绒毛,先出叶可达1/2的倍于叶柄;叶片卵形或狭卵形, 10-18 * 5-10 厘米,纸质,无毛到背面的被绒毛,正面具柔毛的沿脉,基部心形,偏斜,先端锐尖的或锐尖尖;脉9,出现的顶端的对超过基部1-3厘米,其它人基部;网脉明显穗状花序对生。

The slice of pathology showed: the control group of infected the small intestines mucosa grew thiner,mucosa grew swelling and congesting,many lymphocytes infiltrated,villus failed off;The small intestines mucosa didnt swell and congest in the group of the leaf of Psidium guajava,little lymphocytes infiltrated under the mucosa and the villus were well; The small intestines mucosa grew swelling and congesting in the control group of treatment,many lymphocytes infiltrated under the villus.

病理切片展示:感染对照组的小肠粘膜变薄,粘膜肿胀、充血,大量淋巴细胞浸润,绒毛脱落;番石榴叶组的小肠粘膜无明显肿胀、充血,粘膜下少负淋巴细胞浸润,绒毛清楚;治疗对照组小肠粘膜肿胀、充血,粘膜--F人量淋巴细胞浸润。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400

It was expressed on trophoblastic cell, interstitial cell and endothelium cell of interstitial blood vessels of villi.

结果 ①所有的标本绒毛滋养层细胞、绒毛间质细胞、毛细血管内皮细胞都有HAV的存在。

Measure normal early pregnancy choria tissue and JAR cell by RT-PCR, LH/hCG receptor mRNA expression on both pregnancy choria tissue and JAR cell which is consistent with that be measured in ovarian granulosa cells, indicate that JAR cell and normal choria trophocyte presence LH/hCG receptor, hCG is one of the autocrine hormone.

二、用RT-PCR法可测到正常妊娠早孕绒毛组织、JAR细胞的LH/hCG受体mRNA表达,与正常卵巢颗粒细胞所表达的LH/hCG受体mRNA一致,提示JAR细胞与正常绒毛滋养细胞存在LH/hCG受体,hCG为其自分泌激素之一。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

采用荧光定量PCR技术检测EB1基因在人工流产绒毛核型分析正常的胚胎细胞和自然流产绒毛核型分析异常的胚胎细胞中的表达差异;免疫组织化学方法检测EB1蛋白在两者中的表达情况,并用Western blot加以验证。

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