纯化

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

The new-style and rarefied program that develops recently, like filmy biology reactor , attainable very high quality rarefied water, have alexipharmic action and presence of solid of float of extremelying He Xuan, this may be the method that changes this problem.

最近发展的新型纯化程序，如薄膜生物反应器，可得到非常高品质的纯化水，具消毒作用及无任何悬浮固体存在，这可能为改变此问题的方法。

We have purified the fusion protein by use of amylose chromatography and the purity of fusion protein was 91%.

纯化后的融合蛋白可以被兔血清识别，证明纯化后的融合蛋白仍具有较好的反应原性。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

The method to purify compound 4 was resolved. A novel method to prepare the intermediate from phenylacetyl chloride was selected in this paper. The facile synthetic scheme put forward in this work is simple to operate and has the characteristic of high yield and good feasibility.

解决了终产品4的分离纯化难题，采用生成衍生物再进行水解的方法达到了纯化目的；提出了合成中间体3-羟基-4-苯基-吡喃-2，5-二酮(7)的较为新颖的方法，操作简单，各步中间体无需作进一步纯化便可进行下一步反应，从而得到了结构较新颖的终产品4。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.

利用硫酸铵盐析法初步分离果实蝇卵中的蛋白质，以SDS-PAGE蛋白质电泳分析得知，卵黄蛋白主要在硫酸铵溶液浓度为50~70%时被沉淀出；盐析沉淀所得之卵黄蛋白，再以离子交换层析法进一步纯化显示，卵黄蛋白主要会在0.3~0.5 M氯化钠的浓度范围间被析出来；再将析出液浓缩后，经凝胶过滤层析法做最后的纯化，即获得纯化之卵黄蛋白。

The objective was to develop a rapid and simple colloidal gold immunochromatographic assay for the diagnostic of chicken NDV. Tri-sodium citrate with aqueous gold chloride were warmed up and mixed to make gold sol. Colloidal golds were coupled with the purified anti-ND antibody.

为了建立一种快速、简便的检测鸡新城疫病毒的胶体金免疫层析法，采用柠檬酸钠还原法制备胶体金颗粒，标记纯化的ND抗体，并包被在玻璃纤维素膜上，另外将纯化的ND抗体和纯化的兔抗鸡抗体包被在硝酸纤维素膜上，组装成ND快速检测试纸条。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管，4～6h增菌后，加入适量甘油-生理盐水保存液，-20℃冰箱保存；链球菌属和奈瑟氏菌属的菌株纯化后，用接种环取菌置于新鲜脱纤维绵羊血中，加入适量保存液，-80℃低温冰箱保存；真菌菌种纯化后接种于带硅胶塞的半固体培养基，25℃培养24h后，-20℃冰箱保存。

In her eyes, because that is the doorway toher heart * the place where love resides.

女人的漂亮必須從她的眼睛中去看，因為那是她心靈的窗戶和愛居住的地方

I will send some postcards to you.

我会向你发送一些明信片。