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The results are as follows: 1The collagenous fibers are arranged in a crass-crossed network of three dimentions. The network in an area of less than 500 μm is like a small board called a microboard. All microboards anastomose each other, forming a microboard network; 2The so-callcd collagenous fibers or lamellae about 4—9 μm thick under the LM are really th...

结果表明:①胶原纤维排列成三维交叉网状結构,后者在约500μm范围内呈板状,称微板,微板相互吻合成微板网;②在LM下所见的厚约4—9μm的"胶原纤維"或"板层",实际是微板网的垂直切面,⑧文献中的板层结构TEM照片实为其网状结构的一部分,④在胶原纤维之间有少量分支交错的细小纤维,可能是弹性纤维。

This article consists of:(1) a brief review of the previous work concerning this subject,(2) the various methods of studying the arrangement of fibers in yarns, and (3)two basic forms of fiber arrangement——approximate cylindrical helix and approximat conical helix——in ordinary cotton yarns, spun by the roller-drafting system The reasons of formation of such arrangement and their influence on yarn strength are also analyzed.

本文分三部分,第一部分引述了国外以往对细纱中纤维排列形态的研究结果,从而引出本文研究目的;第二部分主要介绍了研究棉纱中纤维排列形态的方法;第三部分根据所采用之方法得出试验结果,表明在一般棉纱中纤维排列成近似圆柱形螺旋线与近似圆锥形的复什的螺旋线二种基本形态。

We identified that in vitro ASM promotes fibrocyte chemotaxis and chemokinesis (distance of migration after 4.5 hours, 31 mum [2.9 mum] vs 17 mum [2.4 mum], P =.0001), which was in part mediated by platelet-derived growth factor (mean inhibition by neutralizing antibody, 16% [95% CI, 2% to 32%], P =.03) but not by activation of chemokine receptors.

该研究首次证明:在哮喘中,成纤维细胞存在于哮喘患者气道平滑肌间隔,并可以促进纤维细胞迁移。成纤维细胞在哮喘患者气道平滑肌增生及气道功能障碍过程的所起的作用仍有待研究。

The electron microscope showed that in the screened sheet, only long fibers interweaved in a crisscross fashion, while in the unscreened sheet, fines fill up the textile fibers, which helped to increase the bonds of long fibers.

扫描电镜显示筛分后所成纸页中只有长纤维纵横交错,而未经筛分的纸页中细小纤维填充在长纤维所交织组成的网络空穴里,有利于纤维间的结合。

The mechanics properties of carbon fiber and the comparison between the two kiwis of paper which consisted of wood fiber and ES fiber respectively were investigated in this paper.

研究碳纤维纸的力学性能,并对比不同配抄纤维所成纸页的力学性能。

Rat dermal fibrobalasts and human neofetus dermal fibroblasts were cultured in vitro to evaluate the biocompatibility of the chitosan films.

为考察壳聚糖对皮肤成纤维细胞的相容性,采用流延法制备了纯壳聚糖膜和含甘油的壳聚糖膜,并在所制备的壳聚糖膜上进行了大鼠皮肤成纤维细胞和人胎儿皮肤成纤维细胞的体外培养。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物;培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感,据此可在传代过程中将二者分离纯化,获得纯细胞系;混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞;再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞,经过2~3 代,即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物;培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感,据此可在传代过程中将二者分离纯化,获得纯细胞系;混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞;再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞,经过2~3 代,即可得到纯化的乳腺上皮细胞系。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性,通过建立猪皮肤成纤维细胞系,将所建细胞系与人胚胎肾293细胞体外共培养,并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验,结果表明,猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中,猪内源性逆转录病霉感染人胚胎肾细胞,进一步证实和拓宽了猪细胞PERV感染人细胞的范畴;猪皮肤成纤维细胞移植SCID鼠皮下后,导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官,但是并未检测出SCID鼠组织中表达PERV env RNA。

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