积累

The major results of this proposal are as follows:(1) ABA induces a rapid, substantial accumulation of apoplastic H2O2 in mesophyll and bundle sheath cells of maize leaves, and the accumulation of apoplastic H2O2 is involved in the induction of the chloroplastic and cytosolic antioxidant enzymes.(2) ABA-induced H2O2 production activates a 46 kDa MAPK, which in turn induces the expression of antioxidant genes and up-regulates the activities of antioxidant enzymes. The activation of MAPK also enhances H2O2 production, forming a positive amplification loop.(3) Water stress also induces the activation of a 46 kDa MAPK, which is dependent on the accumulation of ABA and H2O2 production induced by water stress and involved in the up-regulation of the expression and the activities of antioxidant enzymes.(4) ABA-induced H2O2 production mediates NO generation, which in turn activates a 46 kDa MAPK and results in the up-regulation in the expression and the activities of antioxidant enzymes in ABA signaling. NO-independent signaling is also involved in ABA- and H2O2-induced antioxidant defense.

本项目的主要研究结果如下：（1）ABA诱导的H2O2产生主要出现在叶肉细胞与维管束鞘细胞的质外体中，质外体H2O2的积累能够上调叶绿体与细胞溶质中抗氧化酶的活性；（2）ABA诱导的H2O2产生活化一个46kDa的MAPK，由此而导致编码抗氧化防护酶基因的表达与酶活性的上调；而MAPK的活化也能增强H2O2的产生，从而形成一个正的反馈调节环；（3）水分胁迫也能诱导一46kDa MAPK活化，这一MAPK活化依赖于水分胁迫诱导的ABA积累以及H2O2的产生，同时参与水分胁迫诱导的抗氧化防护基因的表达与抗氧化酶活性的上调；（4）ABA诱导一个依赖于H2O2的NO产生，NO活化一个46kDa的MAPK，从而导致抗氧化防护基因的表达以及抗氧化酶活性的上调；同时一个不依赖于NO的信号转导途径也存在于ABA诱导的抗氧化防护过程中。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Five dominant species including 〖WTBX〗Commelina communis,Elsholtzia haichowensis,Silene fortunei,Polygonum perfoliatum〖WTBZ〗 and 〖WTBX〗Kummerowia striata〖WTBZ〗 were found to have high concentration of total Cu,Pb,Zn,Cd,Cr.

结果表明，鸭跖草等5种植物对重金属Cu、Pb、Zn、Cd、Cr都有程度不等的高量积累，尤其是铜元素，其最高积累量出现在海洲香薷根部，达到844.65 mg/kg，是一般植物Cu含量（5~25 mg/kg）的33.8~1689倍；而在同样条件下，As在5种植物体内的含量为018~098 mg/kg，远远低于As在一般植物中的含量（＜10 mg/kg），这说明这些植物对As可能具有某种规避机制。

Considering the limits of RDSS in active positioning mode,such as poor security,limited capacity,and inability to continuously provide position,velocity and attitude outputs,a technique of tightly coupled RDSS /SINS integrated navigation system is proposed for land vehicles by utilizing the time differenced carrier phase of 3 geostatic satellites.

针对北斗系统有源定位方式保密性差、用户数目有限，不能提供连续的位置、速度、姿态信息的问题，提出利用北斗系统3颗地球静止卫星的载波相位时间差分信息，与车载捷联惯导构成紧组合导航系统，通过扩展卡尔曼滤波器估计并修正惯导系统的速度误差；引入载体的侧向和天向速度约束，改善了速度估计精度；结合北斗系统的伪距信息，消除了长航时条件下位置误差的积累；推导了滤波器观测方程，对组合导航滤波器进行了设计；通过车载实验进行了验证，实验结果表明，速度误差和位置误差的积累受到了有效地抑制，精度满足陆地战车导航的要求。

The effects on yield and canopy biomass accumulation and distribution after anthesis of later sowing wheat were studied in different fertility levels. The results showed that each above ground organs hydrocarbonate accumulation increased with fertility level rising.

对不同肥力水平下晚播小麦开花后地上器官光合产物积累、运转和对产量影响的研究表明，开花后同一天，各地上器官光合产物的积累量均随肥力水平的提高而增加。

At last,this paper analyzed the influence of the inversive receiver to the chracteristic of the clutter and the ratio of signal power and the noise power ,discussed the effective detection of target signal in correlated clutter,and then put forward a new method naming multi-periods shift accumulation for detecting moving target when not knowing the exact movement parameter.

最后，本文分析了倒置接收对目标回波信噪比及杂波特性的影响，讨论了在相关杂波区对回波信号有效检测的方法。针对如何在弹目相对运动参数未知的条件下对高速运动目标进行积累检测这一问题，提出了一种多周期移位积累的新方法。

In conclusion,sex expression of Lagenaria leucantha can be effective-ly modified by phytohormones.

用110m Ag-STS示踪结果表明，乙烯拮抗剂STS在雄蕊里积累的量显著地多于在雌蕊中积累的量。

Different potassium levels have significance influence on nutrient content , accumulating amount , risistance of Lolium. Pereme , but does not have remarkable influence on the content of chlorophyll and dry weight of Lolium. Pereme.

不同钾营养水平对黑麦草的养分含量和积累量、抗逆性有着显著性影响，但对黑麦草的干物质积累和叶绿素含量没有显著的影响。

The direction and the strength of the spin polarization are well controllable and manipulatable by simply varying the strength of spin-orbit interaction or the energy levels in quantum dots.

通过改变自旋轨道耦合强度或量子点内的能级很容易调节自旋极化的强度和方向，而且当量子点内的能级相等时电子-电子互作用能够增强量子点内的自旋积累，当量子点内的能级不相等时电子-电子互作用不能增强量子点内的自旋积累。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。