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碎裂作用

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The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示: VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性;经4 mmol/L VPA处理MUTZ1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞浆内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示:VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性;经4mmol/LVPA处理MUTZ-1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞装内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

In fact, it is a disturbed shear zone blending cataclasite and mudstone under complicated geological process.

受不同地质构造作用的影响,在错动带内部形成夹碎裂岩和泥岩不同构造。

The flexing action of the sleeve not only unplugs the valve, but also breaks up the lime.

套管的弹性作用不仅不会堵塞阀门,还能够碎裂固化的石灰。

Results of HE dye after cerebral alchemic reperfusion showed: Cortex was infiltrated by neutrophil; Cells of corpus striatum and cortex of parietal lobe were stained light; the number off cells obviously reduced; the feature of cells necrosis, such as cells swelling, nuclear fragmentation and lysis et al, would be observed.

HE染色结果显示:脑缺血再灌注后,皮质区中性白细胞浸润,纹状体外侧和顶叶皮质细胞染色淡,细胞数明显 NF-。B在脑缺血再灌注后脑组织局部炎症反应中的作用减少,出现细胞肿胀,胞核碎裂和溶解等细胞坏死特征。

The Wendeng gold deposit is a tectonic breccia cataclastic rock type gold deposit dominated by brittle shear zone and formed by brittle shearing.

文登金矿是受脆性剪切带控制的构造角砾岩碎裂岩型金矿床,是脆性剪切作用形成的,为典型的动力成岩成矿。

Cooperative effect of 5-Fu and TRAIL was also determined. The structure feature of apoptosis cell such as karyopyknosis, karyorrhexis and apoptotic body was found lightmicroscopically and electron- microscopically.

HE染色光镜观察和电镜观察发现经TRAIL作用后的细胞具有核浓缩、碎裂、凋亡小体形成等凋亡的形态特征。

Results HE staining showed that sulindac could inhibit the growth of tumor cells, with the morphological presentations as apoptosis and necrosis including pyknosis, karyorrhexis and chromatin crescent formation, etc. By electron microscopy, cells also had apoptotic characteristics including chromatin crescent formation and apoptotic body. Immunohistochemistry and Western-blot assay results showed that 48 hours after sulindac administration, the expression of NF-κB, bcl-2 and bax gradually decreased, while the level of bax protein gradually increased, in a dose dependence manner.

结果 苏木精-伊红染色检测结果显示舒林酸抑制肿瘤细胞生长,肿瘤细胞出现核固缩、核碎裂、染色质边集等凋亡及坏死的形态学表现;电镜下可看到染色质边集、凋亡小体;免疫组化、Western-blot结果显示舒林酸作用48 h后人胃癌SGC-7901细胞NF-κB、bcl-2表达逐渐下降,bax表达逐渐上升,具有剂量依赖性。

It has been warranted through experiments and practical application that rare-earths-high-aluminium heat-resistant nodular iron has some intrinsic properties which are worthy of reconimandation, namely,that it will not scale after long periods of use at 1050-1100℃, will hardly break while being hammered into castings, runners or risers as the high-temperature tensile strength reaches 14-19 kg/mm2 at 800℃ or≥l3.3kg/mm2 at 900℃, with the room temperature strength close to HT 30-50 and the unnotched impact str...

本文介绍了稀土高铝耐热球墨铸铁的生产实践、化学成分;探讨了稀土作用及其用量和控制;论述了稀土和铝加入方式以及冷却速度对金相组织的影响;并对铸造工艺、铸件缺陷及分析、耐热性能及使用效果作了阐明。试验和使用证明:稀土高铝耐热球墨铸铁在1050~1100℃长时间工作不起氧化皮,热强度800℃时,14~19kg/mm~2,900℃时≥13.3kg/mm~2,室温强度近于HT30—54,a_k≈2~6kg·M/cm~2,锤击铸件或浇冒口,很难碎裂,并能机械加工。可以认为是以我国富产资源为原料,能用各种炉子熔炼、耐热和综合机械性能均较高硅、中硅中铝球铁、高铝片墨铸铁为高的一种耐热铸铁,也是镍铬系耐热材料较好的替代品。

Emodin induced the nuclei of K562 with anomalistic and fragmentized for 96 hours. Agarose gel electrophoresis of DNA revealed a typical DNA ladder map of apoptosis.

大黄素作用96h后,荧光显微镜下可见细胞核不规则,核碎裂呈新月形改变,DNA琼脂糖凝胶电泳显示,基因组DNA显现典型的凋亡细胞的梯状图谱。

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