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Objective: To observe the effects of different cell lysis buffers on protein quantification with Bradford method and bicinchoninic acid method.

目的:观察不同细胞裂解液对Bradford法和bicinchoninic acid法的影响,以确定适合蛋白质组学研究中细胞全蛋白含量的定量方法。

From the starting value selection method in the Fuzzy ISODATA algorithm, used the method of maximal matrix element to ascertain the number of classification, the theoretical analysis of repeated test, and finally, the improved fuzzy ISODATA algorithm is obtained. The algorithm reduced sensitivity to the starting value. The algorithm can highly effective clustering analyze and obtains a stable result, so it presents an efficient way of improving the contribution value of custom service.

研究中先对模糊ISODATA聚类演算法中,初始划分矩阵和分类数的确定,并使用最大矩阵元素法,求得最佳化演算法中其他参数值,最后在CRM系统实际验证和分析中,采用本研究改良之模糊ISODATA聚类演算法,对汽车销售公司实施客户模组分类,经实验证明所得到的聚类结果,可有效解决一般聚类方法受限於参数设定敏感度的困扰,并使聚类精确度提高,有效排除杂讯敏感的影响,使聚类效果大幅提升,可帮助行销人员做出预测,制订出针对客户差异化的行销策略,提高客户服务的贡献价值。

One of a set of measurable factors, such as temperature and pressure, that define a system and determine its behavior and are varied in an experiment.

之一一组可测量的确定某一系统并决定该系统的状况且在实验中是变化的因素之一,例如温度和压力

In reversed micelle the dye showed characteristics of two monomolecular states, the balance between which could be affected by all factors investigated above.

主要研究了反胶束中菁染料的光谱性质,确定了反胶束中菁染料的存在状态,以及反胶束ω值,水团微组份及温度等对菁染料吸收及荧光光谱性质的影响。

PubMed abstract: A psychological space is established for any set of stimuli by determining metric distances between the stimuli such that the probability that a response learned to any stimulus will generalize to any other is an invariant monotonic function of the distance between them.

PubMed摘要∶对于任何一组刺激物的心理学空间的建立,是透过确定刺激物之间的刻度距离,如此,对于任何刺激物之反应而习得的可能性,是一种能够被普遍应用到其他任何对象之间距离上,单一不变的功能。

Immunostaining is a reliable method to identify fungi in infected tissues, it might be used in the diagnosis of deep mycosis.

结论免疫组化为组织中确定真菌和鉴定真菌种属提供了新的可靠方法,有助于临床诊断,因而具有广阔应用前景。

We studied the effects of protein content, pH and ionic strength on viscosity of silver carp myofibril protein by orthogonal rotational regression design, and established the regression equation of silver carp myofibrillar protein solution viscosity with protein content, pH and ionic strengt

利用二次回归正交旋转试验设计,研究了蛋白质浓度、pH、离子强度对鲢鱼肌原纤维蛋白粘度的影响,建立相应的回归方程,分析比较了它们间的相互作用关系,确定了取得最优粘度时,各因素的组

An error detection code is attached on a per transmission symbol basis and transmission is performed, and, when mobile terminal apparatus 100 at the receiving end detects an error, subcarrier information obtainer 130 counts the number of carriers having received level below predetermined standard on a per group basis, and makes the group having the count value above a threshold level subject to retransmission request.

在要发送的每个发送码元上增加差错检测码,当接收端的移动终端装置(100)检测到差错时,副载波信息获得器(130)对具有低于预定基准的接收电平的副载波的数量计数,并且确定重发具有高于门限值的计数值的组。

The third member of Dongying Formation Oligocene (Ed3) is one of the major oil producing pays. As the oilfield developing, the contradiction between distribution of reservoir sandbodies and production practice becomes more serious. For further production, it is the key point to get better understanding of sedimentary characteristic and reservoir distribution.

随着油田开发的不断深入,储集体分布范围及规模与开采动态的矛盾日益凸现,对东三段III油组沉积相分布规律的认识以及砂体的微相类型确定与空间展布已成为制约下一步开发的核心问题。

The advantages of the hybrid CIS-DFT method are that it can determine the symmetry of molecule and the correct order of excitation as well as the Molecular Orbital electron configuration;thereby the electronic excitation states of ethylene are easily derived, and most of them are in agreement with those obtained in experiments or references.

CIS-DFT的优点在于能确定外场下分子的对称性,给出正确的激发顺序以及分子轨道的电子组态,由此导出乙烯分子的激发态,结果与实验一致。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。

The almost sure central limit theorem is a pop topic of the probability research in recent years,because it has many actual applications in the random analogue.

中文摘要:几乎处处中心极限定理是近几十年概率论研究的一个热门话题。它之所以引起人们的注意是由于它在随机模拟方面的实际应用参见Fisher