病毒核心

aim: to study the expression in rat liver and carcinogenesis of hepatitis c virus core protein.

目的：研究丙型肝炎病毒核心蛋白在鼠肝中的表达和可能潜在的直接致癌作用。

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

The researchers found that the central genes or 'backbone' of the H9N2 virus that infects guinea fowl can protect birds and mice against highly pathogenic strains of influenza.

研究员们发现感染几内亚鸟类的H9N2病毒的核心基因或者主干基因可预防鸟类和鼠类感染高致病的流感病毒株。

Objective To explore the role of hepatitis C virus core protein on the infiltration and metabasis of cholangiocarcinoma tissues.

目的 探讨丙型肝炎病毒核心蛋白在胆管癌组织浸润和转移中的作用。

Hepatitis B virus is the smallest DNA virus which is a member of the viral family Hepadnavirida. It uses its DNA as template and the RNA polymerase II of the host cells to transcribe its viral transcripts. There are four major RNAs transcribed from HBV genome. All of the four major transcripts are unspliced, terminated at a common site, polyadenylated at the 3' terminal and capped at the 5' terminal.

B 型肝炎病毒属於肝病毒科，为目前已知最小的去氧核醣核酸病毒，利用其病毒的去氧核醣核酸以及宿主细胞内的核醣核酸聚合酶可转录出四种主要的核醣核酸产物，此四种主要的转录产物均未经剪接并终止於核心开放编阅序列的共同polyadenylation 讯号，且在其5'端均经过capping修饰。

objective to assess the fulfillment of round 3 of global fund aids program in china in order to provide basis for selecting scientific preventive measures and making reasonable work plan.methods to analyze the core programs from reports of round 3 of global fund aids program in 72 counties of 7 provinces in mid part of china,and to assess it by combining specific investigated results.results up to dec 31,2006,fulfilling rate of receiving treatment for occasional infection,and the number of preventing aids patients as well as infected persons was 98.57%.the rate of other index overpassed rearranged objectives.of which,the rate of vct was 111.45%,the rate of receiving anti-virus treatment was 108.42%,the rate of programs activities of government and non-government was 133.83%.at least there was one teacher trained living skills about aids.the fulfilling rate of the number of middle schools of developing living skill education about aids was 125.73%.the fulfilling rate of the number of middle school students educated about living skills was 147.53%.conclusion the fulfillment of objective management of round 3 about aids,especially core index was effective.but there existed the problem that fulfilling rate of some index was too high,which would be corrected in the later phase.

对中国中部7个省72个县实施第三轮全球基金艾滋病项目的完成项目指标报表进行汇总，对其中10个核心项目进行分析。结果截至2006年12月31日，10个核心项目中，除接受机会性感染治疗和预防的艾滋病患者/感染者人数的完成率为98.57%，其他指标完成率均超过了预定目标。其中自愿咨询检测人次数的目标完成率为111.45%；接受抗病毒治疗的艾滋病患者数的目标完成率为121.37%；获得关怀与支持的hiv感染者/艾滋病患者及其家庭数的目标完成率为108.42%；政府部门与非政府机构主动开展的项目活动数的目标完成率为133.83%；至少有一位教师接受过艾滋病生活技能培训，并对中学生开展艾滋病生活技能教育的中学数的目标完成率为125.73%；接受生活技能教育的中学生数目标完成率为147.53%。结论第三轮艾滋病项目的目标管理，特别是核心指标的完成情况取得了比较理想的效果，但存在个别指标完成率过高的问题，将在后续阶段予以纠正。

Objective To study the effects of 20/21 bp partial deletion mutation (from nt 1 748 or nt 1 747 to nt 1 767) in the core promoter region of hepatitis B virus genome complicated by precore stop condon mutation at nt 1 896 on the expression of the viral antigens.

目的为研究乙型肝炎病毒核心启动子20/21bp部分缺失(nt1748/1747至nt1767)及同时存在的A1896点变异对病毒抗原表达的影响。方法利用前期构建的HBV全基因的重组载体转染HepG2细胞后，对病毒抗原进行ELISA检测及Western-blotting分析。

Woodchucks . The HBV genome has four genes: pol, env, pre-core and X that respectively encode the viral DNA-polymerase, envelope protein, pre-core protein (which is processed to viral capsid) and protein X. The function of protein X is not clear but it may be involved in the activation of host cell genes and the development of cancer.

乙型肝炎病毒基因组有四个基因：波兰，环境，前核心和X ，分别编码病毒DNA聚合酶，包膜蛋白，前核心蛋白和蛋白质十大功能蛋白X是尚不清楚，但它可能参与激活宿主细胞的基因和癌的发展。

On December 26, 2005, computational center is in Beijing north software of active to small drop defence undertook series is attacked after defending a test, give height the opinion: By Beijing east small drop finite liability company offers IT ' small drop is active defence software ', with defence to identifying sealed virus has better effect, initiated the new thinking of active defence virus, it is the major breakthrough that turns over virus core technology and innovation.

微点主动防御技术已经得到了国家和有关权威部门的高度关注和支持，2007年12月29日，微点主动防御技术被列为国家&863&科技攻关项目。2005年12月26日，北京北方计算中心在对微点主动防御软件进行了系列攻防测试后，给予高度评价：由北京东方微点信息技术有限责任公司提供的'微点主动防御软件'，对识别和防御未知病毒具有较好的效果，开创了主动防御病毒的新思路，是反病毒核心技术的重大突破和创新。

Detection of HCV core antigen in human serum and plasma with an automated chemiluminescent immunoassay.

丙型肝炎病毒核心抗原检测试剂的研制及初步应用。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。