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Apparent partition coefficient in octanol-water and binding percentage to BSA of ~(153)Sm-NTMP,~(153)Sm-HEDTMP,~(153)Sm-DCTMP,~(153)Sm-EDTMP,~(153)Sm-DTPMP,~(113,117) Sn~m-EDTMP,~(113,117) Sn~m-HEDTMP,~(113,117) Sn~m-DTPMP are measured. The results show that there is a linear relationship between the relative magnitude of the apparent partition coefficient in octanol-watetr and the relative magnitude of the binding percentage to BSA of these ~(153)Sm~(113,117Sn~m complexes.

测定了NTMP,HEDTMPN (羟乙基乙二胺基三亚甲基膦酸,DCTMP(1,2 环己二胺四亚甲基膦酸),EDTMP(乙基二胺基四亚甲基膦酸),DTPMP(二乙基三胺基五亚甲基膦酸)的153Sm配合物以及HEDTMP,EDTMP,DTPMP的113,117Snm配合物在正辛醇水中的表观脂水分配系数和在0 5%牛血清白蛋白水溶液中与BSA的结合率。

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ~, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞,经传代纯化后,脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞,500μg/mLG418筛选2周,G418减半继续培养2~3代,然后对转染筛选后的细胞进行PCR检测及核型分析。

Moisture contained in the tree elements and butter cream to enhance skin and fat of natural protection of the water film, so that the skin soft.

所含的滋润素和牛油树精华,加强皮肤天然保护功能的水脂薄膜,使皮肤柔和。

Aqua, Almond Oil*, Shea Butter*, Plantago*, Cetraria islandica*, Salix alba*, Parfum**(Citral, Geraniol, Citronellol, Limonene, Linalool), Polyglyceryl-3 Stearate, Glycerin, Sodium stearoyl lactylate, Jojoba Oil*, Olive Oil*, Rose Hip Seed Oil*, Bees Wax, Vitamin E, Xanthan gum, Apricot Kernel Oil*, Wheat Germ Oil, Sallow Thorn Oil*, Carrot Seed Oil*, Seaweed Exract, Lactic acid* certified organic ** blend of pure essential oils

有机杏仁油,有机牛油果脂,有机车前,有机冰岛地衣,有机水杨甙,纯天然有机香水(柠檬醛,香叶醇,香茅醇,柠檬烯,芳樟醇),聚甘油-3 硬脂酸酯,甘油,硬脂酰乳酰乳酸钠,有机荷荷巴油,有机橄榄油,有机玫瑰果油,蜂蜡,维他命E,黄原胶,有机甜杏仁油,小麦胚芽油,有机沙棘油*,有机胡萝卜籽油,海藻提取物,乳酸

Affer 4 to 8 weeks, the serum n itric oxide, plasma endotheliolysin, cGMP and lipid peroxide were meas ured and morphologic change of renal tissue examined.

为探讨穿心莲API0134防治家兔系膜增殖性肾炎作用机理,采用高胆固醇饲料另加牛血清白蛋白一次性注射方法建立家兔M sPGN模型,观察4~8周,检测血清一氧化氮,血浆内皮素,环鸟苷酸(cGMP 脂质过氧化物以及肾组织形态学改变。

Aloe vera, Rosehip oil, Glycerin, Almond oil, Cetearyl wheat straw glycosides and cetearyl alcohol, Evening primrose oil, Jojoba oil, Glycerol stearate, Cocoa butter, Avocado oil, Carrot oil, Shea butter, Eyebright, Vitamin E, Grapefruit seed extract, Phenoxyenthanol, Iodopropynol butylcarbamate.

芦荟,野玫瑰果籽油,甘油,杏油,麦秸鲸蜡硬脂基葡糖苷和十六烷基醇,月见草油,霍霍巴油,甘油硬脂酸盐,可可脂,鳄梨油,胡萝卜油,牛油树脂,小米草,维生素E,葡萄籽精华素,苯氧乙醇,碘代丙炔基氨基甲酸丁

Cephalomappa sinensis is a monotypic genus ever-green tree endemic to the calcareous mountainous region in southwest of Guangxi Zhuang Autonomous fresh branches are used as feeding stuff for cattle,horse,sheep,and can make them find the possibility of developing feed additive or medicine for livestock,the chemical constituents from this plant were chemical constituents of its roots had been reported in our earlier continuation of our investigation on the branches of Cephalomappa sinensis,a new norneolignan,named Ceplignan(1),was isolated from ethanol extract through column structure was elucidated on the basis of spectroscopic evidence.

肥牛木(或称肥牛树,Cephalomappa sinensis)为大戟科一单种属植物,为常绿乔木,其分布区狭窄,仅限于广西西南部石灰岩山地[1]。肥牛木为优良木本饲料树种,是牛、马和羊的好饲料,用该植物饲养牛,可使牛肥壮,故有肥牛木的名称[1]。研究其化学成分,目的在于探讨开发饲料添加剂或兽药的可能性。我们曾报道了肥牛木根的化学成分[2,3],在对其枝条的进一步研究中,又分离得到一个新的降新木脂素,命名为肥牛木素。本文报道其分离方法和结构鉴定结果。

In this paper, a kind of moslemic beef sausage, which preserved beef flavor and covered up the disadvantage of palatability of cattle back fat, was developed using beef and cattle back fat as raw materials.

采用牛肉和牛背脂做原料,研究开发出清真牛肉香肠,产品既保持了牛肉原有风味,又掩盖了牛油的膻味和适口性差等缺点。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子,嗜热菌糖苷酶基因lacS为目的基因,构建了lacS基因乳腺特异表达载体,并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞,借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞,为利用核移植技术制备转基因动物提供细胞来源。

METHODS: Lipofectamine method: The BMMSCs were obtained from the tibias and femurs of the guinea pigs. The third passage BMMSCs were cultured with plasmid-lipofectamine mixture for 6 hours, followed by fetal bovine medium for 48 hours. Immunohistochemistry was performed for transient expression. G418 was added after 48 hours.

①脂质体法:取体外分离、培养的第3代豚鼠骨髓间充质干细胞,将质粒-脂质体混合物加入含细胞的培养基中培养6 h,再加入胎牛血清的培养基,孵育48 h 后行免疫组织化学检测,即为瞬时表达。48 h后加入含G418培养基筛选。

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